Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine

A technology for bursal disease and Newcastle disease, applied to medical preparations containing active ingredients, antiviral agents, pharmaceutical formulas, etc., can solve the problems of autoimmune system disorder, not widely promoted, high production costs, etc., to improve body fluid Immunity, improve the ability to prevent diseases, use safe and effective effect

Active Publication Date: 2014-02-19
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the clinical use of live vaccines at home and abroad, although live vaccines can play a part of the protective effect, there is a shrinkage of the bursa of Fabricius itself, which leads to the disorder of the autoimmune system. In addition, genetic engineering vaccines, antigen-antibody complex vaccines There is also a...

Method used

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  • Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine
  • Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine
  • Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Using DF-1 cell line to cultivate chicken infectious bursal virus to prepare chicken infectious bursal disease antigen solution

[0047] (1) Passage and culture of cells for seedling production: take chicken embryo fibroblast passage cell line DF-1 cell line, digest and passage with trypsin cell dispersion liquid, and continue to culture with cell growth liquid. When a good monolayer is formed, it is used to continue passaging or inoculation with virus;

[0048] (2) Propagation of cytotoxic seeds: take the infectious bursal virus HQ strain virus seeds for production, inoculate them in chicken embryo fibroblast passage cells that have grown into a good single layer according to 1% of the maintenance liquid volume, and place them at 37 Continue culturing in maintenance medium at ~38°C, and harvest the cell fluid when the cell lesion rate reaches 75% or more;

[0049] (3) Propagation of venom for seedling production: take the above-mentioned cell line culture f...

Embodiment 2

[0051] Example 2 Preparation of Chicken Newcastle Disease Antigen Solution Using Chicken Embryo Passage Cell Line and Bioreactor Culture

[0052] (1) Selection of bioreactor: Rapid flow reactor AP-20 with a volume of 7L.

[0053] (2) Selection of cells: chicken embryo cell line DF-1, a cell line suitable for the growth of chicken Newcastle disease virus, non-carcinogenic, purchased from the American Type Culture Collection;

[0054] (3) Selection of virus strains: the chicken Newcastle disease virus La sota strain was selected, which can be purchased from the China Veterinary Drug Control Institute.

[0055] (4) Cultivation of different cells: DF-1 cells are cultured in a square bottle, generally passaged at a ratio of 1:3 to 1:5, digested and counted after the cells have grown to a single layer, and used for large-scale cell culture.

[0056] (5) Propagation of cell-adapted virus species: take the cell-adapted Newcastle disease virus virus species, inoculate it into the pass...

Embodiment 3

[0061] Embodiment 3 Preparation of Chinese medicine polysaccharides and Chinese medicine flavonoids

[0062] a. Weigh 20 parts of Achyranthes bidentata, 15 parts of Poria cocos, 25 parts of Andrographis paniculata, 30 parts of Astragalus membranaceus, 20 parts of Banlangen, 20 parts of raw land, 20 parts of angelica, 15 parts of licorice and 20 parts of rhubarb, chop and wash Then soak in cold water overnight, then add purified water 15 times the weight of the raw material, take a water bath to 90°C, and keep it at 90°C, cook for 2 hours, and stir every 10 minutes during the cooking process;

[0063] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0064] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, and the supernatant i...

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Abstract

The invention discloses a preparation method for a newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine and belongs to the technical field of biological products for veterinary use. The preparation method comprises preparation of a newcastle disease antigen liquid, preparation of an infectious bursal disease antigen liquid and preparation of the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine. The vaccine prepared by the invention has a dual characteristic of water in oil and oil in water and has the advantages of integration of a slow release function of the conventional vaccine and the jointed functions of a water-soluble immunopotentiator, Chinese herbal medicinal polysaccharide, an analgesic and the like. According to the preparation method, the immunity of an organism is greatly improved, and the humoral immunity is improved; the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine can stimulate the organism to generate efficient neutralizing antibodies, and the disease prevention ability of the organism is improved. According to the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine, adverse reactions of chickens caused by fractional immunization of multiple vaccines are avoided, the immunization procedure is simplified, and the investment cost is reduced. The vaccine preparation by the invention is suitable for chickens of all ages; one-day chicks are immunized, so that immunity failures of a live vaccine caused by maternal interference resistance or immunosuppression caused by high toxicity can be avoided; the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine is safely and effectively used.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a chicken Newcastle disease and bursal disease dual compound inactivated vaccine. Background technique [0002] Newcastle disease (ND) is a severe viral infectious disease caused by Newcastle disease virus (NDV). It is the most harmful one and is listed as a Class A infectious disease by the International Veterinary Office (OIE). At present, Newcastle disease is still relatively common throughout the country. It restricts the development of my country's poultry industry and the export of poultry products, and also poses potential hazards to food safety and human health. For the control of the disease, in developed countries, hunting of virulent infected groups is the main method, combined with vaccination; while for the majority of developing countries, vaccination is still the main means of controlling the disease. Wit...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/17A61P31/14A61K39/12
Inventor 张秀文李阳闫艳丽刘雪王二先盛璐丝
Owner 浙江美保龙生物技术有限公司
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