Cytokine adsorbent for hemoperfusion and preparation method thereof

A technology of cytokines and adsorbents, applied in the field of cytokine adsorbents and their preparation, can solve problems such as ineffective effects, and achieve high clearance rate, good blood compatibility, and high safety

Active Publication Date: 2014-02-19
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are domestic reports of continuous blood purification being used clinically, but the effect is not obvious [Yu Chen et al., Journal of Nephrology and Dialysis and Kidney Transplantation, 2004,13(5):401]

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 : Preparation of a cellulose-based cytokine adsorbent:

[0030] Step 1: Synthesis of ligand 2-chloro-N-(4-dodecylaniline) acetamide

[0031] Add 100mL (86.6g) of toluene, 13g of p-dodecylaniline, 50mL (50g) of 10% sodium carbonate solution, and dropwise add 6.8g of chlorine at 20-30°C into a three-necked flask equipped with a constant pressure funnel and a stirrer. Acetyl chloride for 1 hour, and then reacted for 5 hours with stirring at 20-30°C. After the reaction is completed, stir to cool, filter, and dry to obtain the product 2-chloro-N-(4-dodecylaniline)acetamide.

[0032] Step 2: Grafting reaction of ligand

[0033] In a 100mL three-necked flask, add 2g of anhydrous potassium carbonate, then add a mixed solution of N,N-dimethylformamide (9mL) (8.5g) and acetone (6mL) (4.7g), then add 0.3mL (0.3 g) Porous cellulose carrier, and tetrabutylammonium bromide 10mg, then add the 2-chloro-N-(4-dodecylaniline) acetamide (0.2g) synthesized above, react at room...

Embodiment 2

[0038] Example 2: Preparation of agarose-based cytokine adsorbent

[0039] Step 1: Synthesis of ligand 2-chloro-N-(4-hexadecylaniline) acetamide

[0040] Add 100mL (86.6g) of toluene, 15.9g of p-hexadecylaniline, 50mL (50g) of 10% sodium carbonate solution, and dropwise add 6.8g of Chloroacetyl chloride for 1 hour, then keep stirring at 20-30°C for 5 hours. After the reaction is completed, stir to cool, filter, and dry to obtain the product 2-chloro-N-(4-hexadecylaniline)acetamide.

[0041] Step 2: Grafting reaction of ligand

[0042] In a 100mL three-necked flask, add 1g of anhydrous potassium carbonate, then add a mixed solution of N,N-dimethylformamide (6mL) (5.7g) and acetone (3mL) (2.4g), then add 0.1mL (0.1 g) Agarose carrier, and tetrabutylammonium bromide 11mg, then add the 2-chloro-N-(4-hexadecylaniline) acetamide (0.2g) synthesized above, react at room temperature for 16h, after the reaction is completed The acetone was distilled off, washed with water and satu...

Embodiment 3

[0047] Example 3: Preparation of cross-linked polyvinyl alcohol-based cytokine adsorbent

[0048] Step 1: Synthesis of ligand 2-chloro-N-(4-octylaniline)acetamide

[0049] Add 100mL of toluene, 10.3g of p-octyl aniline, 50mL of 10% sodium carbonate solution to a three-neck flask equipped with a constant pressure funnel and a stirrer, and add 6.8g of chloroacetyl chloride dropwise at 20-30°C for 1 hour. The reaction was incubated and stirred at ℃ for 5h. After the reaction is completed, stir to cool, filter, and dry to obtain the product 2-chloro-N-(4-octylaniline)acetamide.

[0050] Step 2: Grafting reaction of ligand

[0051] In a 100mL three-necked flask, add 2g of anhydrous potassium carbonate, then add a mixed solution of N,N-dimethylformamide (9mL) (8.5g) and acetone (6mL) (4.7g), then add 0.3ml (0.3 g) Cross-linked polyvinyl alcohol carrier, and tetrabutylammonium bromide 10mg, then add the 2-chloro-N-(4-octylaniline) acetamide (0.25g) synthesized above, react at ro...

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Abstract

The invention discloses a cytokine adsorbent for hemoperfusion and a preparation method thereof. The cytokine adsorbent for the hemoperfusion comprises a hydrophilic carrier used as an adsorbent substrate and a hydrophobic ligand fixed on the surface of the carrier by using chemical crosslinking, wherein the hydrophobic ligand is long chain pair alkylaniline with 4-18 carbon atoms and a derivant thereof. The cytokine adsorbent for the hemoperfusion has the advantages of good cytokine adsorption effect, high safety, low cost and the like.

Description

technical field [0001] The invention relates to a cytokine adsorbent and a preparation method thereof, in particular to a cytokine adsorbent for blood perfusion and a preparation method thereof. Background technique [0002] The mortality rate of patients with sepsis (Sepsis) and septic shock (Septic Shock) is between 25% and 80%. According to research reports, even in the United States, which has the most advanced medical technology, there are new cases of sepsis every year. More than 750,000 patients were diagnosed with the disease, of which more than 210,000 died (Crit Care Med 2001;29:1303–1310). [0003] Sepsis is an intricate physiological-pathological reaction process with network characteristics involving a series of inflammatory and immune cells and various inflammatory mediators, cytokines, coagulation substances, and oxygen free radicals derived from them. In the progression of the disease, this physiological-pathological reaction process amplification is called ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/26B01J20/28B01J20/30
Inventor 董凡
Owner JAFRON BIOMEDICAL
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