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Tobacco residue solid organic waste decomposing inoculant and preparation method thereof

A technology of organic waste and solids, applied in the field of microorganisms, can solve the problems of propagation, time-consuming decomposition, poor nicotine degradation effect, etc.

Active Publication Date: 2014-03-19
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the existence of strong phytotoxic nicotine in tobacco waste, although it contains rich organic matter, it cannot be directly applied to the soil as an organic fertilizer. Composting alone takes a long time to decompose, the degradation effect of nicotine is poor, and it will not only cause environmental pollution. Pollution and waste of resources, may also lead to the spread of breeding diseases and pests

Method used

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  • Tobacco residue solid organic waste decomposing inoculant and preparation method thereof
  • Tobacco residue solid organic waste decomposing inoculant and preparation method thereof
  • Tobacco residue solid organic waste decomposing inoculant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 strain screening

[0025] Cellulose Degrading Bacteria Screening Medium: CMC-Na5g, KH 2 PO 4 1g, urea 0.5g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O7.5mg, MnSO 4 ·H 2 O2.5mg, ZnSO 4 ·7H 2 O3.6mg, CoCl 2 ·6H 2 O3.7mg, CaCl 2 0.5g, 0.02% Congo red, 16g agar, dilute to 1L with deionized water.

[0026] Beef extract peptone medium: beef extract 5g, peptone 10g, NaCl 5g, solid plus agar 16g, used for the isolation and cultivation of bacteria.

[0027] PDA medium: Potato (peeled) 200g, sucrose (or glucose) 20g, solid plus agar 16g, used for the isolation and cultivation of fungi.

[0028] Congo red agar medium is a good medium for separation and screening. Cellulose-degrading bacteria can form a transparent circle on the red plate, and the ratio of transparent circle / colony diameter roughly reflects its ability to degrade cellulose. The two screened strains with the ability to degrade cellulose were named N1 and N2. It can be seen fr...

Embodiment 2

[0031] Example 2 strain identification

[0032] N1 and N2 strains were identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis.

[0033] The N1 strain is Gram-positive, straight and short rod-shaped, with peripheral flagella, able to move, and the size is 0.7μm~0.8μm×2.0μm~3.0μm. After culturing on nutrient agar solid medium plate for 24 hours, the colony shape is round or nearly round, the surface is rough and opaque, stained white or yellowish, often forming wrinkles, and the size of the colony is 2mm to 3mm. After 16srRNA gene sequence analysis and homology comparison, it was known that it had 99% homology with strain HG328254.1 in GenBank, so it was identified as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and named Bacillus amyloliquefaciens N1. The strain has been deposited in the General Microbiology Center of the National Microbiological Culture Collection Management Committee, the pres...

Embodiment 3

[0035] Embodiment 3 nicotine resistance test

[0036] 1 Test of nicotine resistance of strains

[0037] According to the nicotine content in the waste smoke, set the nicotine concentration to 0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, set three repetitions for each gradient, add different concentration gradient nicotine into the 30mL beef extract peptone medium in a 100mL Erlenmeyer flask. The seed liquid of the strain to be tested was inoculated into the culture medium at an inoculum amount of 1%, at 30°C and 170r / min, and after culturing for 24h, the OD value was measured with a UV spectrophotometer at a wavelength of 600nm.

[0038] Add the same amount of seed solution of N1 and N2 strains to the prepared medium with nicotine concentration of 0%, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%, and culture with shaking at 30°C and 170r / min for 24h Afterwards, the absorbance was measured with a spectrophotometer at a wavelength of 600 nm, which was used to determine the resistance concentration o...

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Abstract

The invention discloses a nicotine resistant strain N1 preserved in China General Microbiological Culture Collection Center on September 9, 2013 with a preservation number of CGMCC8143, and application of the strain N1 in degrading tobacco residue solid organic waste. The invention also discloses a nicotine resistant strain N2 preserved in China General Microbiological Culture Collection Center on September 9, 2013 with a preservation number of CGMCC8144, and application of the strain N2 in degrading tobacco residue solid organic waste. The invention further discloses a tobacco residue solid organic waste decomposing inoculant including the strain N1 and the strain N2, and discloses a preparation method of the tobacco residue solid organic waste decomposing inoculants. The steps include: fermenting the strain N1 and the strain N2, and compounding the fermentation liquids of the strain N1 and the strain N2 to obtain the decomposing inoculant.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial agent for decomposing smoke solid organic waste. Background technique [0002] my country's tobacco planting area and output both rank first in the world. At present, tobacco at home and abroad is mainly used as cigarettes. As a major tobacco producing country, my country's tobacco planting area is about 1.3 million ha 2 , with an annual output of 4.5-5 million tons. In the process of cigarette processing and production, a large amount of leftover waste such as tobacco leaves and smoke foam will be produced. The annual total amount of tobacco waste in my country is about 900,000 to 1 million tons, accounting for about 25% of the total output, most of which are discarded, not only It causes a waste of resources and seriously pollutes the environment. [0003] Smoke waste refers to the part of tobacco stems other than the leaves with curing value, including tobacco stems...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/08C12R1/07C12R1/32
Inventor 李想刘艳霞石俊雄
Owner GUIZHOU TOBACCO SCI RES INST
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