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Corn steep liquor enzymatic hydrolyzate is used in the production method of microbial oil fermentation

A microbial oil and production method technology, applied in the direction of fermentation, etc., can solve the problems of decreased effective utilization rate of fermentation tanks, increased risk of fermentation contamination, unfavorable microbial utilization, etc. The effect of improved utilization

Inactive Publication Date: 2016-02-17
山东悦翔生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the amino acids in corn steep liquor exist in the form of macromolecular proteins, it is not conducive to the utilization of microorganisms
In order to ensure the normal progress of microbial fermentation, a large proportion of corn steep liquor needs to be used. With the increase of the application amount of corn steep liquor, macromolecular proteins and other colloidal substances will produce more foam during the fermentation process, and the effective utilization rate of the fermentation tank will decrease. In addition, insoluble particles in corn steep liquor and some microbial spores that are not easy to be killed by high temperature increase, and the risk of fermentation contamination increases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of enzymatic hydrolyzate of corn steep liquor:

[0017] Take 5000 g of corn steep liquor with a solid content of 48%, add water to dilute to a solid content of 19%, and adjust the pH to 3.8 with 0.2N hydrochloric acid. Add phytase and acid protease respectively in diluent, the mass percentage of described phytase amount accounting for dry matter in corn steep liquor is 0.032%, and the mass percentage of described acid protease accounting for dry matter in corn steep liquor is 1.8%. Keep the enzymolysis temperature at 48°C-50°C, the stirring speed at 200r / min, and the time for 18h. After enzymatic hydrolysis, the temperature was raised to 90°C for 25 minutes to inactivate the enzyme. After the enzyme inactivation reaction, the temperature was lowered to 20° C., and centrifuged at 4500 rpm for 15 minutes, and the supernatant was taken for later use.

[0018] Medium formula (g / 100ml):

[0019] Sodium chloride 1.24g, potassium chloride 0.07g, calcium chloride...

Embodiment 2

[0026] Preparation of enzymatic hydrolyzate of corn steep liquor:

[0027] Take 5000 g of corn steep liquor with a solid content of 47.5%, add water to dilute to a solid content of 20%, and adjust the pH to 3.7 with 0.2N hydrochloric acid. Add phytase and acid protease respectively in the diluent, the amount of phytase accounted for 0.03% by mass percent of dry matter in corn steep liquor, and the amount of acid protease accounted for 1.6% by mass percent of dry matter in corn steep liquor. Keep the enzymolysis temperature at 50°C, the stirring speed at 200r / min, and the time for 18h. After enzymatic hydrolysis, the temperature was raised to 90°C for 20 minutes to inactivate the enzyme. After the enzyme inactivation reaction, the temperature was lowered to 20° C., and centrifuged at 4500 rpm for 15 minutes, and the supernatant was taken for later use.

[0028] Medium formula (g / 100ml):

[0029] Sodium chloride 1.35g, potassium chloride 0.065g, calcium chloride 0.04g,

[00...

Embodiment 3

[0036] Preparation of enzymatic hydrolyzate of corn steep liquor:

[0037]Take 5000g of corn steep liquor with a solid content of 40-50%, add water to dilute to a solid content of 18%, and adjust the pH to 3.5 with 0.2N hydrochloric acid. Add phytase and acid protease respectively in the diluent, the amount of phytase accounted for 0.03% by mass percent of dry matter in corn steep liquor, and the amount of acid protease accounted for 1.5% by mass percent of dry matter in corn steep liquor. Keep the enzymolysis temperature at 48°C, the stirring speed at 210r / min, and the time for 16h. After enzymatic hydrolysis, the temperature was raised to 90°C for 20 minutes to inactivate the enzyme. After the enzyme inactivation reaction, the temperature was lowered to 20° C., and centrifuged at 4500 rpm for 15 minutes, and the supernatant was taken for later use.

[0038] (1) Bacteria activation culture

[0039] The slant medium formula is as follows:

[0040] Potato 30g, glucose 2-4g,...

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PUM

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Abstract

The purpose of the present invention is to provide corn steep liquor enzymatic hydrolysis instead of corn steep liquor, which is used for microbial oil fermentation production, so as to meet the needs of the market. Using phytase and acid protease to enzymatically hydrolyze corn steep liquor, the enzymatic hydrolyzate obtained by centrifugation replaces corn steep liquor as the medium, which effectively reduces the risk of bacterial contamination. The content of inorganic phosphorus / total phosphorus in the enzymatic hydrolyzate is 75-90%, Microbial bioavailability is improved, production costs are reduced, and in microbial oil fermentation, it is conducive to the rapid accumulation of unsaturated fatty acids, shortening the fermentation cycle, and reducing microbial fermentation costs.

Description

technical field [0001] The invention relates to the field of microbial oil fermentation, in particular to the enzymolysis treatment of corn steep liquor as a culture medium for microbial oil fermentation. Background technique [0002] Corn steep liquor is a by-product of preparing corn starch. Corn kernels are first soaked with sulfurous acid, and the soaking liquid is heated and evaporated to concentrate the yellowish-brown liquid. Corn steep liquor contains soluble protein, auxin and some precursor substances, containing about 40 -50% solid matter. Corn steep liquor is a widely used organic nitrogen source for microbial growth. Because it is rich in organic nitrogen and trace elements, it is widely used in the fermentation industry. It can also promote the biosynthesis of penicillin and other antibiotics. Since most of the amino acids in corn steep liquor exist in the form of macromolecular proteins, it is not conducive to the utilization of them by microorganisms. In or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
Inventor 简晓红刘真茂李金龙邱顺江
Owner 山东悦翔生物有限公司
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