Method for extracting heparin sodium from small intestine of pig and co-producing protein powder

A technology of heparin sodium and protein powder, applied in the field of heparin sodium extraction, can solve the problems of wasting resources, polluting the environment, large water consumption, etc., achieving the effects of high equipment utilization rate, reducing environmental pollution, and increasing added value

Active Publication Date: 2014-03-26
SHIFANG LELI FUYANG BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the content of heparin sodium in the animal lung is high, due to its tissue specificity, there are a large amount of impurity protein, and the protein in the mast cells of the lung tissue binds to heparin sodium very firmly, making it difficult to separate and purify, and difficult to use
Pig small intestinal mucosa is mostly used as raw material for the production of heparin sodium in my country. An invention patent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for extracting heparin sodium from pig small intestine and co-producing protein powder adopts the following steps:

[0027] (1) Enzyme hydrolysis and salt hydrolysis

[0028] Wash 500 pig small intestines, collect 550Kg of fresh mucous membrane, add 825Kg of water, stir well, the pH value is 8.0, add 85Kg of edible salt, stir well, heat up to 28°C, add 0.2kg2079 alkaline For protease, heat up to 45°C, add 0.3kg 2079 alkaline protease, stir evenly, then adjust the pH to 8.5, adjust the salinity to Baume 4.5, keep warm at 50°C for 2.5 hours, heat up to 95°C, and keep warm for 10 minutes.

[0029] (2) Protein isolate powder

[0030] After filtering the product obtained in step (1) with 60-mesh nylon cloth, the filter residue was washed with 1:1 85°C water; then filtered with 80-mesh nylon cloth, and 180kg of crude protein powder was obtained after pressing, and the protein powder content was 40%, the combined filtered filtrate was used to extract heparin sodi...

Embodiment 2

[0036] The method for extracting heparin sodium from pig small intestine and co-producing protein powder adopts the following steps:

[0037] (1) Enzyme hydrolysis and salt hydrolysis

[0038] Take 1000 pig small intestines and wash them, collect 1100Kg of fresh mucous membranes, add 2000Kg of water, stir evenly, adjust the pH value to 8.5, add 180Kg of edible salt, stir evenly, heat up to 32°C, add 0.4kg2079 Alkaline protease, heat up to 46°C, add 0.7Kg2079 alkaline protease, stir well, then adjust the pH to 8.5, adjust the salinity to Baume degree 4.5, keep it at 50°C for 1.5 hours, adjust the pH value to 8.5, and keep it for 1 hour. Heat up to 95°C and keep warm for 10 minutes;

[0039] (2) Protein isolate powder

[0040] Filter the product obtained in step (1) with 60-mesh nylon cloth, and wash the filter residue with 1:1 85°C water; then use 80-mesh nylon cloth to filter and squeeze to obtain 480kg of crude protein powder, and its protein powder content was 43%, and th...

Embodiment 3

[0046] The method for extracting heparin sodium from pig small intestine and co-producing protein powder adopts the following steps:

[0047] (1) Enzyme hydrolysis and salt hydrolysis

[0048] Take 1500 pig small intestines and wash them, collect 1650Kg of fresh mucous membranes, add 2500Kg of water, stir well, adjust the pH value to 8.0, add 250kg of edible salt, stir well, heat up to 30°C, add 0.55kg of 2079 base Protease, heat up to 48°C, add 1kg 2079 alkaline protease, stir evenly, then adjust the pH to 8.5, adjust the salinity to Baume 4.2, keep it at 52°C for 1.5 hours, adjust the pH value to 8.5, and keep it for 1 hour. Heat up to 90°C and keep warm for 8 minutes;

[0049] (2) Protein isolate powder

[0050] Filter the product obtained in step (1) with 60-mesh nylon cloth, and wash the filter residue with 1:1 80°C water; finally filter the filtrate with 100-mesh nylon cloth, and press the filter residue to obtain 529kg of crude protein powder. The powder content is 4...

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Abstract

The invention relates to a method for extracting heparin sodium, and particularly relates to a method for extracting heparin sodium from a small intestine of a pig and co-producing protein powder. The method comprises steps of (1) carrying out enzymolysis and salt hydration; (2) separating protein powder out; and (3) extracting heparin sodium: adsorbing, eluting, precipitating and drying. The method improves the yield of heparin sodium, furthermore, the equipment for co-production has higher usage rate than single producing equipment, saves resources, and can lower the production cost of heparin sodium and protein powder. The small intestine of the pig is fully used and is improved in additional value, furthermore, the pollution of waste liquid and waste residue containing protein to environment is reduced, and the waste liquid and waste residue are turned into wealth.

Description

technical field [0001] The invention relates to a method for extracting heparin sodium, in particular to a method for extracting heparin sodium by using pig small intestines. Further, the invention can extract heparin sodium and co-produce protein powder at the same time. Background technique [0002] Heparin sodium is the sodium salt of aminodextran sulfate extracted from animal viscera. It belongs to mucopolysaccharides and can be used as an anticoagulant. Clinical studies have shown that in addition to its anticoagulant effect, heparin sodium also has a variety of other biological activities and clinical applications. It can treat acute venous thrombosis and acute coronary syndrome, angina pectoris, myocardial infarction, antithrombotic, and lower blood lipids. Thrombus and static and dynamic thrombus formation, without affecting platelet aggregation and fibrinogen binding to platelets, is also used to reduce the recurrence of cancer-associated venous thrombosis. [0003...

Claims

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Application Information

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IPC IPC(8): C08B37/10C12P21/06
Inventor 杨三蓉刘亚兰
Owner SHIFANG LELI FUYANG BIOLOGY CO LTD
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