Method for extracting heparin sodium from small intestine of pig and co-producing protein powder

Abstract

The invention relates to a method for extracting heparin sodium, and particularly relates to a method for extracting heparin sodium from a small intestine of a pig and co-producing protein powder. The method comprises steps of (1) carrying out enzymolysis and salt hydration; (2) separating protein powder out; and (3) extracting heparin sodium: adsorbing, eluting, precipitating and drying. The method improves the yield of heparin sodium, furthermore, the equipment for co-production has higher usage rate than single producing equipment, saves resources, and can lower the production cost of heparin sodium and protein powder. The small intestine of the pig is fully used and is improved in additional value, furthermore, the pollution of waste liquid and waste residue containing protein to environment is reduced, and the waste liquid and waste residue are turned into wealth.

Description

technical field [0001] The invention relates to a method for extracting heparin sodium, in particular to a method for extracting heparin sodium by using pig small intestines. Further, the invention can extract heparin sodium and co-produce protein powder at the same time. Background technique [0002] Heparin sodium is the sodium salt of aminodextran sulfate extracted from animal viscera. It belongs to mucopolysaccharides and can be used as an anticoagulant. Clinical studies have shown that in addition to its anticoagulant effect, heparin sodium also has a variety of other biological activities and clinical applications. It can treat acute venous thrombosis and acute coronary syndrome, angina pectoris, myocardial infarction, antithrombotic, and lower blood lipids. Thrombus and static and dynamic thrombus formation, without affecting platelet aggregation and fibrinogen binding to platelets, is also used to reduce the recurrence of cancer-associated venous thrombosis. [0003...

AI Technical Summary

Problems solved by technology

Although the content of heparin sodium in the animal lung is high, due to its tissue specificity, there are a large amount of impurity protein, and the protein in the mast cells of the lung tissue binds to heparin sodium very firmly, making it difficult to separate and purify, and difficult to use

Pig small intestinal mucosa is mostly used as raw material for the production of heparin sodium in my country. An invention patent published by someone once disclosed a preparation method for producing heparin sodium using pig small intestine. In the enzymatic hydrolysis step, the weight ratio of intestinal mucosa to water is 1: 7 to 9, the water consumption is so large that it is not practical for large-scale production. At the same time, the filter residue is discarded during the production process, which pollutes the environment and wastes resources.