A kind of production process, preparation and production device of nematocide fungus

Abstract

The invention discloses an Actinomyces Israeli production technology, an Actinomyces Israeli preparation and an Actinomyces Israeli production apparatus. The production technology adopts a liquid-solid biphasic cultivation method to realize the mass production of Actinomyces Israeli, liquid fermentation is carried out to obtain a large amount of mycelia and blastospores and a small amount of conidiospores, and the above obtained substances are transferred to a solid cultivation medium to generate a large amount of aerial conidiospores. An Actinomyces Israeli pill or pulvis prepared through the technology is convenient to store, and can be dissolved in a 0.1M sodium citrate solution or is diluted by adding water when the pill or pulvis is used. The production apparatus comprises a disc body, the base plate of the disc body is punched with a plurality of holes, and at least one metal screen covering all the holes is fixed on the base plate. The apparatus guarantees the oxygen and humidity required by the solid cultivation of the Actinomyces Israeli, effectively prevents the living contaminants, and substantially improves the sporulation quantity of the Actinomyces Israeli.

Description

technical field [0001] The invention belongs to the technical field of microbial insecticides, and in particular relates to a production process of nematocide fungi and its preparation and production device. Background technique [0002] Pine wood nematode is also called pine wilt disease. is a devastating epidemic of pine trees. It has strong pathogenicity, multiple transmission routes, hidden disease sites, and fast host death; it spreads quickly and is often caught off guard; once it occurs, it is difficult to treat. Known as cancer of the pine tree. According to incomplete statistics, in the 10 years since the disease occurred in my country in 1982, the area of ​​occurrence reached about 38000hm2. 2 , resulting in the death of more than 1,400,000 pine trees and a loss of 50,000m of timber 3 . The funds for disease prevention and control also reached 6.45 million yuan. It not only causes huge losses to the national economy, but also destroys the natural landscape an...

AI Technical Summary

Problems solved by technology

During the solid fermentation process of nematicidal fungus ehrlichii, the temperature has a great influence on it. Properly raising the temperature is beneficial to the solid fermentation of nematicidal fungus ehrlichii and can increase the amount of spore production, but the heat release of solid fermentation process, it is easy to cause the internal heat of the solid medium to exceed 31 degrees, and it is easy to burn the colony to death, which is not conducive to sporulation

The nematocide fungus is aerobic, so increasing the ventilation in an appropriate amount will help to increase the growth rate of the spores, but the ventilation rate should not be too large during solid fermentation, because the growth rate will be faster, the internal heat will increase, and the temperature is not suitable control, resulting in reduced spore production

Humidity also has a great influence on the solid fermentation of the nematocide fungi. The greater the humidity in the early stage of solid fermentation, the greater the spore production. Large, the spores continue to germinate, and the spore production will inevitably decrease

Due to the specificity of the nematocide fungus, the traditional solid fermentation container shows many disadvantages, which can easily lead to the failure of solid fermentation and the contamination of miscellaneous bacteria, and the rapid contamination of miscellaneous bacteria may even cause high spore content. The product becomes a waste product, so the container for the solid culture of the nematocide fungi must also be improved

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