Stain for degrading ethanethiol, as well as culture method and application of strain

Abstract

The invention discloses a strain for degrading ethanethiol as well as a culture method and application of the strain. The strain is named as Pseudomonas sp. WL2; the preservation number is CGMCC NO. 7898; the strain is preserved in the Common Microorganism Center of the Chinese Microorganism Strain Preservation Administration Committee(CGMCC), and the preservation date is 8th June, 2013. The strain for degrading ethanethiol is an aerobiotic Gram-negative bacterium, which can be grown by taking ethanethiol as a sole carbon source and energy, and can be thoroughly mineralized into CO2 and H2O. Under a pure culture condition, the strain is capable of degrading ethanethiol under the conditions that the temperature is 25-30 DEG C and the pH is 6-8. The strain has good substrate adaptive capacity and substrate extensiveness, and is capable of degrading propanethiol and methanol. The strain establishes the basis for industrial application in treating sulfur-containing stink organic waste gases by using a biologic method.

Description

technical field [0001] The invention relates to the field of microbial strains, in particular to a strain for degrading ethanethiol, a cultivation method and application thereof. Background technique [0002] At present, the common odorous sulfur-containing organic compounds in the petrochemical industry, such as methyl mercaptan, ethanethiol, methyl sulfide, propylene mercaptan, and dimethyl disulfide, have the characteristics of low odor threshold and high toxicity, which are harmful to human health. And the influence of the surrounding environment is becoming more and more significant. However, so far, the pollution control of sulfur-containing organic compounds such as mercaptans and sulfides has been seldom mentioned at home and abroad. As the most typical odor pollutant in the petrochemical industry, the efficient removal of sulfur-containing odor organic pollutants has become a major difficulty in the field of organic waste gas treatment. [0003] Ethyl mercaptan (E...

AI Technical Summary

Problems solved by technology

As a key factor affecting the deodorization efficiency of biological treatment devices, the screening and isolation of specific microbial strains that can efficiently degrade sulfur-containing organic pollutants such as mercaptans and thioethers is still a technical gap in the current research field

[0005] The Chinese invention patent with the authorized announcement number CN 101234280B (application number 200710031286.X) discloses an absorption-biological treatment method for malodorous waste gas, using spray absorption tower and biological trickling filter device as key treatment technologies to carry out hydrogen sulfide-containing , mercaptans, dimethyl sulfide and dimethyl disulfide and other gaseous odor pollutants can be discharged up to the standard; however, the invention mainly carried out process research and did not degrade the specific odorous organic compounds Separation, identification and analysis of strains and degradation characteristics

[0006] In 2012, Chen Liang from Central China Normal University (Master's thesis: Screening of ethanethiol rapid deodorizing bacteria and optimization of its culture medium) etc. took 20ppm ethanethiol as the target pollutant, and carried out the screening and degradation ability of related rapid deodorizing bacteria analysis, the maximum removal rate was lower than 50%; although several strains of Bacillus strains were obtained in this study, their degradation ability was poor, and the application value in the actual industrial process was not great

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