Stain for degrading ethanethiol, as well as culture method and application of strain

A culture method, ethanethiol technology, applied in the direction of microorganism-based methods, separation methods, chemical instruments and methods, etc., can solve the problems of low removal rate, isolation, identification, analysis and application value of bacterial strains and degradation characteristics, etc. To achieve the effect of efficient degradation

Active Publication Date: 2014-03-26
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a key factor affecting the deodorization efficiency of biological treatment devices, the screening and isolation of specific microbial strains that can efficiently degrade sulfur-containing organic pollutants such as mercaptans and thioethers is still a technical gap in the current research field
[0005] The Chinese invention patent with the authorized announcement number CN 101234280B (application number 200710031286.X) discloses an absorption-biological treatment method for malodorous waste gas, using spray absorption tower and biological trickling filter device as key treatment technologies to carry out hydrogen sulfide-containing , mercaptans, dimethyl sulfide and dimethyl disulfide and other gaseous odor pollutants can be discharged up to the stan

Method used

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  • Stain for degrading ethanethiol, as well as culture method and application of strain
  • Stain for degrading ethanethiol, as well as culture method and application of strain
  • Stain for degrading ethanethiol, as well as culture method and application of strain

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Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: Isolation and identification of bacterial strains:

[0045] 1. Domestication and breeding of strains

[0046]Activated sludge taken from the sewage outlet of a pharmaceutical factory (in September 2012, Xinchang Pharmaceutical Factory of Zhejiang Pharmaceutical Co., Ltd. located in Xinchang County, Zhejiang Province) was exposed to the target pollutant (ethanethiol) into the activated sludge. After the air acclimatization is stable (at least 1 month), use a 300mL culture bottle in a constant temperature shaker, add 1mL of activated sludge, 50mL of inorganic salt medium and 10-20μL of target pollutants, and enrich for several cycles (3-5 times) to obtain microbial communities that can degrade the target pollutants; use R2A solid medium (10-fold dilution coating method, streaking method) to purify and isolate degradable strains, that is, to divide the microbial communities by 10 times with sterile deionized water Dilution method for serial dilution, take 10...

Embodiment 2

[0056] Example 2: Degradation properties of Pseudomonas sp. WL2

[0057] 1. Degradation characteristics of Pseudomonas sp. WL2 under different temperature conditions (25-35°C):

[0058] Using ethanethiol as the sole carbon source of Pseudomonas sp. WL2, inoculate 3mL logarithmic phase bacterial suspension (OD 600nm =0.115) in 100mL inorganic salt medium (initial pH=6.7) and placed in a 630mL sealed culture bottle (two parallel samples and one blank control group were designed under each temperature condition), and ethanethiol was added to make the initial pH in the system The concentration is 13.5mg / L, respectively placed in 25, 30, 35 ℃, 150r / min constant temperature shaker for continuous culture, sampling and analysis at regular intervals (the results are as follows image 3 ). This embodiment illustrates that when the initial concentration of ethanethiol is 13.5mg / L, the degradation characteristics when the temperature is 25°C are significantly better than 25°C and 35°C; ...

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Abstract

The invention discloses a strain for degrading ethanethiol as well as a culture method and application of the strain. The strain is named as Pseudomonas sp. WL2; the preservation number is CGMCC NO. 7898; the strain is preserved in the Common Microorganism Center of the Chinese Microorganism Strain Preservation Administration Committee(CGMCC), and the preservation date is 8th June, 2013. The strain for degrading ethanethiol is an aerobiotic Gram-negative bacterium, which can be grown by taking ethanethiol as a sole carbon source and energy, and can be thoroughly mineralized into CO2 and H2O. Under a pure culture condition, the strain is capable of degrading ethanethiol under the conditions that the temperature is 25-30 DEG C and the pH is 6-8. The strain has good substrate adaptive capacity and substrate extensiveness, and is capable of degrading propanethiol and methanol. The strain establishes the basis for industrial application in treating sulfur-containing stink organic waste gases by using a biologic method.

Description

technical field [0001] The invention relates to the field of microbial strains, in particular to a strain for degrading ethanethiol, a cultivation method and application thereof. Background technique [0002] At present, the common odorous sulfur-containing organic compounds in the petrochemical industry, such as methyl mercaptan, ethanethiol, methyl sulfide, propylene mercaptan, and dimethyl disulfide, have the characteristics of low odor threshold and high toxicity, which are harmful to human health. And the influence of the surrounding environment is becoming more and more significant. However, so far, the pollution control of sulfur-containing organic compounds such as mercaptans and sulfides has been seldom mentioned at home and abroad. As the most typical odor pollutant in the petrochemical industry, the efficient removal of sulfur-containing odor organic pollutants has become a major difficulty in the field of organic waste gas treatment. [0003] Ethyl mercaptan (E...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/48C12R1/38
CPCY02A50/20Y02P20/59
Inventor 李伟王向前王俏丽徐百龙李素静
Owner ZHEJIANG UNIV
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