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Holothurian culture water quality restoration agent for effectively removing water eutrophication and preparation method and application thereof

A technology for water eutrophication and aquaculture water quality, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. Mortality and other issues, achieve good suspension and controllability of sinking and floating, avoid acidification and smelly bottom, and reduce stress response

Inactive Publication Date: 2014-04-02
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the current situation in the prior art, such as serious pollution of aquaculture water quality, frequent occurrence of diseases, high mortality rate of sea cucumbers, excessive heavy metals, chemical residues and antibiotic residues in sea cucumbers, and the like, the present invention invents a sea cucumber aquaculture water that can effectively remove eutrophication of water bodies. Restorative agent and its preparation method and use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of probiotic stock solution

[0037] Add Bacillus subtilis YB-1CGMCCNo.5116, Bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, which grow well on LB agar slant medium, respectively to 2116E medium, pH7.0~7.5 , shake culture at 37°C for 12-16 hours, and then mix it at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×10 8 cfu / ml.

[0038] 2. Liquid fermentation culture

[0039] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0040] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0041] 3. Spray drying treatment

[0042] The composite ads...

Embodiment 2

[0045] 1. Preparation of probiotic stock solution

[0046] Add Bacillus subtilis YB-1CGMCCNo.5116, Bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, which grow well on LB agar slant medium, respectively to 2116E medium, pH7.0~7.5 , shake culture at 37°C for 12-16 hours, and then mix them at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0047] 2. Liquid fermentation culture

[0048] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0049] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0050] 3. Spray drying treatment

[0051] The adsorption car...

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PUM

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Abstract

The invention discloses a holothurian culture water quality restoration agent for effectively removing water eutrophication and a preparation method and application of the holothurian culture water quality restoration agent, belonging to the technical field of aquaculture and marine microorganisms. Aiming at the physiological habit and culture characteristic of holothurian, a marine microorganism technology is applied, the restoration agent consists of bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 separated and screened from a holothurian culture environment, corn meal and soya bean meal serving as major culture raw materials are fermented at the temperature of 28-37 DEG C for 24-48 hours, and attapulgite, medical stone and rice hull serving as adsorption and suspension composite carriers are used for processing to obtain the holothurian culture water quality restoration agent for effectively removing water eutrophication. The product can adsorb harmful substances such as heavy ions including Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+>, and Cu<2+> in water bodies, and the content of harmful substances such as ammonia nitrogen, nitrite, and hydrogen sulfide can be greatly reduced. Bottom odor and acidification of water bodies are avoided, pathogenic microorganisms are inhibited effectively, holothurian diseases are prevented, and the holothurian culture yield and quality are improved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and marine microorganisms, and in particular relates to a water quality restoration agent for sea cucumber culture that can effectively remove eutrophication of water bodies, its preparation method and application. Background technique [0002] Seawater is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects the growth status of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people's demand for sea cucumbers is increasing day by day. Under the circumstances that natural fishing cannot meet the market demand, the sea cucumber farming industry has transformed from the traditional extensive management and resource-dependent farming methods to the current intensive and factory farming methods. W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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