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Thermus and application thereof

A technology for blackening caused by Thermus and water pipes, which is applied in the field of microorganisms for removing sulfur-containing substances, can solve the problems of increasing treatment costs and increasing energy consumption, and achieves the effects of shortening the start-up period, short adaptation period and good treatment.

Inactive Publication Date: 2014-04-02
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, not only energy consumption is increased, but also a large amount of newly generated waste water needs to be treated, which increases the treatment cost

Method used

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  • Thermus and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The DNA of the target strain was extracted and purified by the automatic magnetic bead nucleic acid extractor Smart LabAssist-16 and the corresponding environmental sample DNA extraction and purification kit, respectively, and PCR amplification was carried out with bacterial general primers F16S-27 and R16S-1492. The primer sequences were respectively for:

[0041] F16S-27 (5`-AGAGTTTGATCCTGGCTCAG-3`)

[0042] R16S-1492 (5`-CGGTTACCTTGTTACGACTTC-3`)

[0043] The PCR reaction conditions were set as follows: 94°C pre-denaturation for 5 min; followed by 94°C denaturation for 30 sec, 56°C annealing for 30 sec, 72°C extension for 90 sec, and 25 cycles; then 72°C extension for 10 min; and finally 4°C for 10 min. The PCR product was then ligated to the PMD-19T vector, and then transformed into Escherichia coli DH5α competent cells. Spread 50 μl of transformed competent cells on LB medium containing galactoside, isopropyl-β-d-thiogalactoside and ampicillin. After culturing ...

Embodiment 2

[0044] Example 2: Treatment of waste gas with thermus bacterium agent

[0045] Centrifuge the fermented Thermus agrigenans ZDL1 and resuspend the bacterial agent obtained by nutrient solution in a bioreactor, feed the gas containing sulfur and malodorous substances, and purify the sulfur-containing substances in the exhaust gas with Thermus agrigenans ZDL1 Malodorous substances, thermus bacteria agent volume is 1m 3 , the content of ZDL1 of Thermus agrigenus in water pipes was 10 10 CFU / ml (悬浮液) , the total concentration of sulfide is 150ppm, and the gas flow rate is 10m 3 / h, and the purification temperature is 60°C.

[0046] The composition of described nutrient solution is: beef powder 5.0g, KH 2 PO 4 2.0g, KNO 3 2.0g, NH 4 Cl0.5g, MgCl 2 ·6H 2 O0.5g, FeCl 2 ·7H 2 O0.01g, NaHCO 3 1.0g, 1000ml of water, pH 6.5; track and detect the degradation ability of sulfur-containing malodorous substances.

[0047] The present invention adopts gas chromatography to track an...

Embodiment 3

[0049] Example 3: A microbial active filler for removing sulfur-containing substances and purifying medium / high temperature sulfur-containing waste gas

[0050] The porous material is attached with a suspension of Thermus aeruginosa ZDL1;

[0051] The porous material is granular activated carbon;

[0052] The density of the porous material is 0.8kg / m 3 , the porosity is 86%, the particle size is 20mm;

[0053] The Thermus aeruginosa ZDL1 is attached to the granular activated carbon in the form of bacterial suspension;

[0054] The mass ratio of the Thermus aeruginosa ZDL1 bacterial suspension to the granular activated carbon is 100:10;

[0055] The cell concentration of Thermus aeruginosa ZDL1 in the microbial active filler is 5.0×10 6 CFU / g (活性填料) .

[0056] The preparation steps of the microbial active filler are as follows:

[0057] (1) Slope culture: culture and prepare the slant strain of Thermus aeruginosa ZDL1;

[0058] The slant medium consists of: beef powder ...

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Abstract

The invention discloses a Thermus strain and application thereof in removing sulfur-containing substances and purifying medium / high-temperature sulfur-containing waste gases. The Thermus scotoductus ZDL1 is an isolate from a medium / high-temperature bioreactor for treating sulfur dioxide and other sulfur-containing waste gases in a water pollution control laboratory at Research Center for Eco-Environmental Sciences of Chinese Academy of Sciences; and the strain is collected to China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7430. The strain can be prepared into a microbial active filler for removing sulfides in medium / high-temperature waste gases and purifying gases, thereby solving the problems of medium / high-temperature malodorous substances and waste gas pollution generated in the process of sewage treatment, sludge drying incineration, refuse incineration, composting or the like; and the degradation efficiency for sulfur dioxide, carbon bisulfide and other sulfur-containing substances can reach higher than 80%.

Description

Technical field: [0001] The invention belongs to the field of environmental treatment, and in particular relates to a microorganism for removing sulfur-containing substances and its application. Background technique: [0002] In the process of sewage treatment, sludge drying and incineration, composting, etc., waste gas with high temperature (35-100°C) will be produced. This medium / high temperature waste gas has complex components, among which sulfur dioxide is one of the main substances. The exhaust gas containing sulfur dioxide escapes into the atmosphere and spreads everywhere with the flow of air, polluting the surrounding environment and endangering human health. At present, the treatment of exhaust gas has attracted more and more attention. Many countries have formulated relevant emission standards to strictly control the emission of pollutants in exhaust gas, and require enterprises to take corresponding treatment measures before emission. my country's national "Comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/85B01D53/50B01D53/48C12R1/01
CPCY02A50/20
Inventor 李琳刘俊新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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