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Preparation method for low-protein and high-purity sea cucumber polysaccharide

A sea cucumber polysaccharide and high-purity technology is applied in the field of preparation of low-protein and high-purity sea cucumber polysaccharide, and can solve the problems of low protein removal efficiency and large loss of polysaccharide.

Active Publication Date: 2014-04-16
山东海普盾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of low protein removal efficiency and large loss of polysaccharides in the preparation of polysaccharides by Sevage method and trichloroacetic acid method in the prior art, the present invention provides a low-protein high-purity sea cucumber with reasonable process, advanced technology and feasible operation Preparation method of polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of low-protein high-purity sea cucumber polysaccharide, through the following process steps:

[0019] (1) Selection and processing of raw materials Select dried sea cucumbers as raw materials, crush them, and pass through a 200-mesh sieve to obtain sea cucumber powder for later use;

[0020] (2) Degreasing treatment Add the sea cucumber powder obtained in step (1) into n-hexane that is 6 times the mass of the sea cucumber powder, and extract and degrease for 8 hours;

[0021] (3) Removal of saponins: The sea cucumber powder after the degreasing treatment in step (2) is suction-filtered to remove n-hexane, and then 80% ethanol is added for extraction treatment for 36 hours to remove saponins; wherein, the amount of ethanol is the amount of sea cucumber after degreasing treatment. 14 times the mass of powder;

[0022] (4) One-time enzymatic hydrolysis Remove ethanol from the sea cucumber powder after saponin removal in step (3), vacuum dry, place in ...

Embodiment 2

[0029] A preparation method of low-protein high-purity sea cucumber polysaccharide, through the following process steps:

[0030] (1) Selection and processing of raw materials Select dried sea cucumbers as raw materials, crush them, and pass through a 300-mesh sieve to obtain sea cucumber powder for later use;

[0031] (2) Degreasing treatment Add the sea cucumber powder obtained in step (1) to petroleum ether 10 times the mass of the sea cucumber powder, extract and degrease for 6 hours;

[0032] (3) Removal of saponins Centrifuge the sea cucumber powder after degreasing treatment in step (2) to remove petroleum ether, add 85% ethanol for extraction treatment for 24 hours, and remove saponins; wherein, the amount of ethanol is the degreasing treatment. 6 times the mass of sea cucumber powder;

[0033] (4) One enzymatic hydrolysis: filter the sea cucumber powder after saponin removal in step (3) to remove ethanol, vacuum dry the sea cucumber powder, put it in enzymatic hydrol...

Embodiment 3

[0040] A preparation method of low-protein high-purity sea cucumber polysaccharide, through the following process steps:

[0041] (1) Selection and processing of raw materials Select dried sea cucumbers as raw materials, crush them, and pass through a 100-mesh sieve to obtain sea cucumber powder for later use;

[0042] (2) Degreasing treatment Add the sea cucumber powder obtained in step (1) to petroleum ether which is 4 times the mass of the sea cucumber powder, and extract and degrease for 10 hours;

[0043] (3) Removal of saponins Centrifuge the sea cucumber powder after the degreasing treatment in step (2) to remove petroleum ether, add 60% ethanol for extraction treatment for 48 hours, and remove saponins; wherein, the amount of ethanol is the degreasing treatment. 20 times the mass of sea cucumber powder;

[0044] (4) One-time enzymatic hydrolysis: filter the sea cucumber powder after saponin removal in step (3) to remove ethanol, vacuum dry the sea cucumber powder and ...

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Abstract

The invention relates to a preparation method for low-protein and high-purity sea cucumber polysaccharide. The method comprises the following steps: selecting dry sea cucumber as raw materials, crushing and adding n-hexane or ligarine for extraction and degreasing processing; removing an organic solvent from degreased sea cucumber powder, and adding ethanol for extraction to remove saponin; drying and placing in an enzymolysis tank, adding water, adding animal protein hydrolytic enzyme and trypsin for one-time enzymolysis after full swelling, and performing enzyme deactivation; performing centrifugation on obtained sea cucumber enzymatic hydrolysate, placing an ultrafiltration liquid obtained through ultrafiltration in the enzymolysis tank, adding flavor proteolytic enzyme for enzymolysis processing, and performing enzyme deactivation; adjusting the pH value, cooling at lower temperature, standing, performing centrifugation and ultrafiltration, collecting an interception liquid, and drying to obtain the dry product of the sea cucumber polysaccharide. The preparation method is reasonable in process, advanced in technology, and practicable in operation. The sea cucumber polysaccharide has the protein content lower than 0.5 percent according to tests.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a method for preparing sea cucumber polysaccharides with low protein and high purity. Background technique [0002] We know that sea cucumber polysaccharide is an important component of the sea cucumber body wall. A large number of documents have confirmed that sea cucumber polysaccharide has various biological activities such as anticoagulant, antitumor, enhancing immunity, and lowering blood lipids. Therefore, it has shown good application prospects in the development of functional foods and marine drugs. [0003] Sea cucumber polysaccharides are connected to proteins by glycopeptide bonds in the body wall of sea cucumbers. Protein is the main impurity that affects the quality of sea cucumber polysaccharides. It is the key to prepare high-purity sea cucumber polysaccharides to reduce the protein content in the product by appropriate methods. There are many ways to remove ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 袁文鹏张绵松胡炜刘昌衡孙永军孟秀梅王志伟鞠文明唐晓波毕春雨
Owner 山东海普盾生物科技有限公司
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