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Bacterial strain for degradation of p-xylene and culture method and application thereof

A technology for paraxylene and a culture method, which is applied to the field of paraxylene degrading strains and their culture, can solve the problems of rareness, culture conditions, optimized analysis of separation and purification methods, and no treatment of xylene organic waste gas, etc. The effect of strong substrate adaptability, good substrate breadth, and efficient degradation ability

Active Publication Date: 2014-04-30
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the current research at home and abroad, there are few reports on the isolation and identification of efficient degradation strains for organic waste gas containing p-xylene
In 2010, Amelia-Elena Rotaru (FEMS Microbiology Ecology, 2010,71:460-468) isolated microorganisms that can use p-xylene as the sole carbon source and electron donor at the same time from the denitrification bacteria in the anaerobic environment of tap water Bacteria; In 2012, Wei Xin of Wuhan University of Science and Technology (Chemical Environmental Protection, 2012, 6:498-501) isolated a strain of Pseudomonas that can use p-xylene as the only carbon source from a petrochemical wastewater activated sludge , for the treatment of petrochemical wastewater and coking wastewater; however, the above studies did not optimize the culture conditions, separation and purification methods of related strains, and did not involve the treatment of organic waste gas containing p-xylene

Method used

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  • Bacterial strain for degradation of p-xylene and culture method and application thereof
  • Bacterial strain for degradation of p-xylene and culture method and application thereof
  • Bacterial strain for degradation of p-xylene and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Isolation and identification of bacterial strains:

[0043] 1. Domestication and breeding of bacterial strains:

[0044] The activated sludge taken from the sewage outlet of the pharmaceutical factory (March 2011, Zhejiang Puluo Kangyu Biopharmaceutical Co., Ltd., Dongyang City, Zhejiang Province) was introduced into the target pollutant (p-xylene) for aeration and domestication (at least 1 months), and use a 300mL culture bottle in a constant temperature shaker to add 1mL of activated sludge, 50mL of inorganic salt medium and 10-20μL of target pollutants (p-xylene), enrich for several cycles (3-5 times) , to obtain the microbial community that can degrade the target pollutant (p-xylene); use R2A solid medium (10-fold dilution coating method, streaking method) to purify and isolate the degradable strain, that is, to use sterile deionized water to deionize the microbial community Serial dilution was carried out according to the 10-fold dilution method, and...

Embodiment 2

[0054] Example 2: Degradation characteristics of Pandoraea sp. WL1

[0055] 1. Degradation characteristics of Pandoraea sp. WL1 under different temperature conditions (25-35°C):

[0056] Using p-xylene as the sole carbon source of Pandoraea sp. WL1, inoculate 3mL of logarithmic phase bacterial suspension (OD 600nm =0.235) in 100mL inorganic salt medium (initial pH=6.5) and placed in a 630mL sealed culture bottle (two parallel samples and one blank control group were designed under each temperature condition), and p-xylene was added to make the system initially The concentration is 41.0mg / L, respectively placed in 25, 30, 35 ℃, 150r / min constant temperature shaker for continuous culture, sampling and analysis at regular intervals (results are as follows image 3 ). This example shows that at a relatively high initial concentration of p-xylene (41.0mg / L), the degradation characteristics at a temperature of 35°C are significantly better than those at 25°C and 30°C; among them, ...

Embodiment 3

[0063] Example 3: Pandoraea sp. WL1 was inoculated into a biological trickling filter device to treat simulated organic waste gas containing p-xylene

[0064] Using the bio-trickling filter device and method disclosed in the Chinese patent application No. 201210019232.2, Pandoraea sp. WL1 was inoculated into the bio-trickling filter device to treat organic waste gas containing p-xylene, and acclimated to In the stable stage, the simulated organic waste gas is prepared with p-xylene as the target pollutant, and the suspension of Pandoraea sp. Simulated organic waste gas treatment (results such as Figure 7). The results showed that the bio-trickling filter reached the stable operation stage after about 14 days of acclimatization process. In the early stage of film acclimatization (1-10 days), the intake concentration is maintained at 200-800mg / m 3 Within the range, the removal efficiency of p-xylene is unstable (50% to 80%); in the late stage of film acclimation, maintain it...

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Abstract

The invention discloses a bacterial strain for degradation of p-xylene and a culture method and application thereof, the bacterial strain is named as Pandoraea sp WL1, the bacterial strain is preserved in the China General Microbiological Culture Collection Center(CGMCC), wherein the accession number is CGMCC NO.7897, and the accession date is July 8th, 2013. The bacterial strain for the degradation of the p-xylene is aerobic-type gram-negative bacteria, can grow by use of the p-xylene as a unique carbon source and energy, and can be completely mineralized into carbon dioxide and water; under the pure culture condition, the bacterial strain can degrade the p-xylene under the conditions of the temperature of 25-35 DEG C and the pH value of 4 to 8; the bacterial strain has good adaptive capacity to a substrate and the broadness to the substrate, can simultaneously degrade the p-xylene and methylbenzene. The bacterial strain lays a foundation for the industrial application of a biological method for disposing organic waste gases.

Description

technical field [0001] The invention relates to the field of microbial strains, in particular to a strain for degrading p-xylene, a cultivation method and application thereof. Background technique [0002] At present, the environmental problems and potential health problems caused by the low-concentration organic waste gas produced by the fine chemical industry are attracting increasing attention. Volatile organic compounds (VOCs) produced in the fine chemical industry have been regarded as a successor to dust due to their wide variety, wide distribution, high toxicity, wide pollution area, large gas volume, and low concentration. At the same time, the problems caused by pollution are complicated. The second largest category of air pollutants with a large amount and a wide range in the future. According to industry calculations, the current national total industrial VOCs annual emissions should be over 20 million tons, reaching or even exceeding the national NO x The level...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01
CPCY02A50/20Y02P20/59
Inventor 李伟王向前王俏丽徐百龙李素静
Owner ZHEJIANG UNIV
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