A kind of production method of bamboo spring handicraft
A production method and handicraft technology, applied in decorative arts, special decorative structures, organic chemistry, etc., can solve problems such as harmfulness to the human body, moldy spots, and rot
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Embodiment 1
[0017] Example 1: Preparation of the compound of the present invention:
[0018] Place benzoxazole (0.1mol) in tert-butanol, then add 0.001mol of X-Phos(2-cyclohexylphosphino-2',4',6'- in the presence of 0.001mol of potassium carbonate) Triisopropylbenzene), and in the presence of 0.002 mol of lead acetate, 0.11 mol of 2-amino-4,6-lutidine was added. In an argon atmosphere, the reaction mixture was heated at 90°C and stirred for 10 hours. It was concentrated under reduced pressure, and the residue was diluted with ethyl acetate. The organic phase was then washed twice with water, dried over magnesium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether:ethyl acetate=3:1, volume ratio) to obtain 13.38 g of the compound of the present invention as a white solid powder.
[0019] 1 H NMR (500MHz, DMSO): δ2.47 (s, 3H, CH 3 ),2.51(s,3H,CH 3 ), 6.36~7.90(m,6H,CH), 8.36(s,1H,N-H).
Embodiment 2
[0020] Example 2: Toxicity test of the compound of the present invention:
[0021] The PPARδ activity of the compounds of the present invention was confirmed by transfection detection. In addition, the selectivity of PPAR subtypes PPARα and PPARγ was also tested. The cytotoxicity is tested by MTT detection, and the in vivo activity is studied by animal experiments.
[0022] CV-1 cells are used in this test. The cells were inoculated into a 96-well plate containing DMEM supplemented with 10% FBS, DBS (non-specific bovine serum, defatted) and 1% penicillin / streptomycin, and heated at 37°C, 5% CO 2 In an incubator. The experiment was carried out according to the steps of inoculation, transfection, sample application and confirmation. Specifically, CV-1 cells were seeded into a 96-well plate (5000 cells / well), and transfected 24 hours later. The full-length PPAR plasmid DNA, reporter DNA that can confirm PPAR activity because of its luciferase activity, β-galactosidase DNA that pro...
Embodiment 3
[0028] Example 3: Antiseptic activity test:
[0029] The antimicrobial efficacy of the compounds of the present invention is evaluated by a challenge test method or artificial contamination.
[0030] Use 5 pure microbial cultures.
[0031] bacterial Secondary medium T° ATCC Escherichia coli (Ec) Trypto-casein soy35℃8739 Enterococcus faecalis (Ef) Trypto-casein soy35℃33186 Pseudomonas aeruginosa (Pa) Trypto-casein soy35℃19429 Candida albicans (Ca) Sabouraud35℃10231 Aspergillus niger (An) malt 35℃6275
[0032] ATCC = American Type Culture Collection.
[0033] The gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), respectively, one day before inoculation (for bacteria and yeast) and five days before inoculation (for mold) The positive bacteria (Enterococcus faecalis), yeast (Candida albicans) and mold (Aspergillus niger) were inoculated in the next generation medium. On the day of vaccinati...
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