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Method for producing 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing carbon dioxide in microorganisms

A technology of microorganisms and hydroxypropionic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as high cost, consumption of petroleum resources, environmental pollution, etc., achieve fast growth and alleviate greenhouse benefits , the effect of reducing pollution

Active Publication Date: 2014-05-21
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, 3-hydroxypropionic acid, acrylic acid and propionic acid are mainly synthesized by chemical methods. However, the current chemical synthesis methods not only have high costs, but also cause environmental pollution and consume precious petroleum resources.

Method used

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  • Method for producing 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing carbon dioxide in microorganisms
  • Method for producing 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing carbon dioxide in microorganisms
  • Method for producing 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing carbon dioxide in microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Escherichia coli producing 3-hydroxypropionic acid

[0063] The Msed_1993 gene fragment on the plasmid pYW4 was used Xba I and Hin Digested with the dIII enzyme and connected to the back end of the Msed_0709 gene on pYW1 to construct a plasmid that simultaneously expresses the two genes Msed_0709 and Msed_1993, named pZL31, see appendix Figure 8 .

[0064] The plasmid pMSD8 constructed by Professor John Cronan's research group can overexpress acetyl-CoA carboxylase ACC in Escherichia coli (Davis M S, Solbiati J, Cronan J E. Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli . Journal of Biological Chemistry, 2000, 275(37): 28593-28598.). In this example, this plasmid was used to increase the amount of malonyl-CoA.

[0065] Co-transform pMSD8 and pZL31 into Escherichia coli BL21 (DE3), use kanamycin and carbenicillin to screen the successful transformants, pick a single clone and culture i...

Embodiment 2

[0074] Example 2 Escherichia coli producing acrylic acid

[0075] The Msed_1993 gene fragment on the plasmid pYW4 was used Xba I and Hin Digested with the dIII enzyme and connected to the back end of the Msed_2001 gene on pYW7 to construct a plasmid that simultaneously expresses four genes Msed_0709, Msed_1456, Msed_2001 and Msed_1993, named pZL32, see appendix Figure 9 .

[0076] The plasmid pMSD8 constructed by Professor John Cronan's research group can overexpress acetyl-CoA carboxylase ACC in Escherichia coli (Davis M S, Solbiati J, Cronan J E. Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli . Journal of Biological Chemistry, 2000, 275(37): 28593-28598.) In this example, this plasmid was used to increase the amount of malonyl-CoA.

[0077] Co-transform pMSD8 and pZL32 into Escherichia coli BL21 (DE3), use kanamycin and carbenicillin to screen the successful transformants, pick a single clone and cul...

Embodiment 3

[0086] Example 3 Escherichia coli producing propionic acid

[0087] The Msed_1426 gene fragment on the plasmid pYW5 was used Xba I and xho After the I enzyme was cut off, it was connected to the back end of the Msed_2001 gene on pYW7, and a plasmid that simultaneously expressed the four genes Msed_0709, Msed_1456, Msed_2001 and Msed_1426 was constructed, named pZL33, see appendix Figure 10 .

[0088] The Msed_1993 gene fragment on the plasmid pYW4 was used Xba I and Hin Digested with the dIII enzyme and connected to the back end of the Msed_1426 gene on pZL33 to construct a plasmid that simultaneously expresses five genes Msed_0709, Msed_1456, Msed_2001, Msed_1426 and Msed_1993, named pZL34, see appendix Figure 11 .

[0089] The plasmid pMSD8 constructed by Professor John Cronan's research group can overexpress acetyl-CoA carboxylase ACC in Escherichia coli (Davis M S, Solbiati J, Cronan J E. Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fa...

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Abstract

The invention discloses a method for producing 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing carbon dioxide in microorganisms, and belongs to the fields of the renewable energy sources, the biomass energy and chemical raw material production. The method comprises the steps of expressing one or more of malonyl-Co A reductase, malonic semialdehyde reductase, 3-hydroxypropionyl-Co A reductase, 3-hydroxypropionyl-Co A dehydratase and acrylyl-Co A reductase genes in microorganisms and producing 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing carbon dioxide. The method is capable of synthesizing 3-hydroxypropionic acid, acrylic acid and propanoic acid directly with carbon dioxide by modifying the microorganisms without chemical synthesis with petroleum resources, and therefore, the consumption of petroleum and pollution on the environment are reduced, and the method is a renewable-energy, low-consumption and environment-friendly technique, and is capable of mitigating the greenhouse effect. The production of 3-hydroxypropionic acid, acrylic acid and propanoic acid by immobilizing the carbon dioxide in the microorganisms can be directly applied to industrial production.

Description

[0001] technical field [0002] The invention belongs to the fields of renewable energy, biomass energy and chemical raw material production, and specifically relates to a method for producing 3-hydroxypropionic acid, acrylic acid and propionic acid by fixing carbon dioxide in microorganisms. Background technique [0003] Almost all autotrophic organisms in the biological world obtain the required carbon source through the process of carbon fixation. The fixation of carbon dioxide in the atmosphere is very important and is considered to be one of the most important life processes on earth. The ways to fix carbon dioxide that have been discovered so far are: Calvin cycle, reducing citric acid cycle, reducing acetyl-CoA cycle and 3-hydroxypropionic acid cycle, in 2007, Georg Fuchs research group in archaea Metallosphaera sedula A fifth way of carbon dioxide fixation was found in: 3-hydroxypropionate / 4-hydroxybutyrate cycle (Berg I A, Kockelkorn D, Buckel W, et al. A 3-hydroxyp...

Claims

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Application Information

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IPC IPC(8): C12P7/52C12P7/42C12P7/40C12N15/70C12N15/81C12N1/19C12N1/21C12R1/865C12R1/19
CPCC12N9/0008C12N9/88C12N9/93C12P7/52C12Y102/01075C12Y402/01116C12Y602/01036Y02P20/59
Inventor 刘天罡柳志杰王艺璇邓子新
Owner WUHAN UNIV
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