A kind of horse milk with hypolipidemic acid prepared by mixed fermentation of compound strains and preparation method thereof

A technology of mixed fermentation and compound strains, applied in the field of microbial fermentation, to achieve the effect of wide practicability and development value

Active Publication Date: 2016-01-06
新疆天牛乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation of functional horse milk by multi-strain compound fermentation at home and abroad. It is of great significance to research and develop practical microbial strains to obtain stable horse milk with blood lipid-lowering function

Method used

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  • A kind of horse milk with hypolipidemic acid prepared by mixed fermentation of compound strains and preparation method thereof
  • A kind of horse milk with hypolipidemic acid prepared by mixed fermentation of compound strains and preparation method thereof
  • A kind of horse milk with hypolipidemic acid prepared by mixed fermentation of compound strains and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment one: Kluyveromyces marxe ( Kluyveromyces marxianus ) Screening, separation, purification and identification of WWMJ-1CGMCCNo.8432

[0056] Primary screening: 1 mL of traditional fermented horse milk is put into a test tube equipped with 9 mL of sterile saline, and fully mixed evenly on a vortex mixer. Dilute the fermented sour horse milk in 10-fold steps, and the dilution is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 . Use a pipette to draw 1mL of each of the above dilutions, pour into the YPD medium by the pouring method, and place the plate in an incubator at 28°C for 24-48h. Observe and record the characteristics of the colonies and pick out suspected yeast colonies, and number the suspected strains.

[0057] Purification of strains: Inoculate the suspected strains on YPD agar medium again, culture them at a constant temperature in a 28°C incubator for 24-48h, and repeat 3-4 times to purify the yeast. The purified yeast strains were inoculated on ...

Embodiment 2

[0063] Embodiment two: the selection of culture medium

[0064] MRS medium: glucose 20g, peptone 10g, beef extract 10g, yeast extract 5g, diamine citrate 2g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween-801mL, distilled water 1000mL.

[0065] YPD medium: yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000mL.

[0066] Wort medium: malt extract powder 130g, chloramphenicol 0.1g, distilled water 1000mL.

[0067] The solid medium of each of the above-mentioned mediums adopts the corresponding addition of 20.0 g / L of agar.

[0068] Kluyveromyces marx ( Kluyveromyces marxianus) Physiological and biochemical test medium of WWMJ-1CGMCCNo.8432: Nitrate reduction medium: 3g of bovine extract, 5g of peptone, 1g of potassium nitrate, 1000mL of distilled water, dissolve each component in distilled water, adjust the pH to 7.2-7.4, and pack into test tubes , about 5mL per tube, sterilized at 121°C for 15min...

Embodiment 3

[0069] Example 3: Mixed fermentation of compound strains to prepare sour horse milk

[0070] See attached figure 1 , using self-selected Kluyveromyces marx ( Kluyveromyces marxianus ) WWMJ-1 and selected Lactobacillus casei ( Lactobacillus casei ), Lactococcus lactis ( Lactococcus lactis ) and Monascus ( Monascus ruber ) to prepare the method for sour horse milk by fermenting the composite strain of the composition, the specific preparation method steps are as follows.

[0071] (1) Horse milk pretreatment: filter fresh horse milk to remove impurities and foreign matter in horse milk, then preheat horse milk to 60-65°C, homogenize at 20MPa for 10-15min, and after homogenization, horse milk Milk is pasteurized and used for fermentation; the amount of mother’s fermented liquid, fermented liquid 1, and fermented liquid 2 that needs to be pretreated is 3:20:20 by volume.

[0072] (2) Preparation of seed fermentation liquid and mother fermentation liquid.

[0073] Seed f...

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Abstract

The invention discloses a method for preparing sour horse milk by mixed fermentation of compound strains and the prepared sour horse milk. Using self-selection Kluyveromyces marx ( Kluyveromyces? marxianus ) WWMJ-1? CGMCC? Based on No.8432, combined Lactococcus lactis ( Lactococcus? lactis ), Lactobacillus casei ( Lactobacillus? casei ) and Monascus ( Monascus? ruber ) to form a composite strain for mixed fermentation, and to test the effects of multiple single-factor experiments on the inoculation ratio, fermentation temperature, fermentation time, and sugar addition amount on fermented horse milk, and use the response surface method to optimize and determine the optimal preparation process parameters of horse milk. The prepared horses' milk has remarkable blood fat-lowering effect, not only retains the rich nutrition in the horses' milk, but also endows the horses' milk with a health care function, and has wide application value.

Description

technical field [0001] The present invention relates to the technical field of microbial fermentation, in particular, the present invention relates to the technical field of preparing functional horse milk by mixed fermentation of microbial strains. Background technique [0002] Horse milk is a treasure in the food culture of ethnic minorities in Xinjiang. Horse milk has high nutritional value. Compared with other livestock milk, it is rich in protein, lactose, vitamins and minerals, and contains essential amino acids and fatty acids for the human body. Horse milk is rich in a variety of nutrients and is easily absorbed and utilized by the human body. It is especially suitable for infants and the elderly and the weak. [0003] Sour horse milk is one of the special foods of ethnic minorities in Xinjiang, which is deeply loved by everyone. Horse milk is rich in a variety of nutrients, which are easily absorbed and utilized by the human body. It also has the functions of nour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23C9/127C12R1/645
Inventor 武运王璐刘武军杨海燕王小标苗森高蕾周建中古丽娜孜黄文书夏俊芳
Owner 新疆天牛乳业有限公司
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