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Method for preparing molecularly imprinted polymer for fluorescence detection of cyhalothrin

A technology of cyhalothrin and molecular imprinting, applied in chemical instruments and methods, other chemical processes, etc., can solve the problem of few composite fluorescent molecular imprinting materials, and achieve the effect of high sensitivity and strong signal

Inactive Publication Date: 2014-06-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the combination of organic fluorescent dyes and molecular imprinting technology to prepare composite fluorescent molecularly imprinted materials.

Method used

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  • Method for preparing molecularly imprinted polymer for fluorescence detection of cyhalothrin
  • Method for preparing molecularly imprinted polymer for fluorescence detection of cyhalothrin
  • Method for preparing molecularly imprinted polymer for fluorescence detection of cyhalothrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Dissolve 0.1 mmol of the template molecule cyhalothrin, 0.0105 g of allyl fluorescein and 0.4 mmol of the functional monomer (AM) in 30 mL of acetonitrile, ultrasonically oscillate, and pre-polymerize at room temperature for 12 hours to fully interact with the functional monomer and cyhalothrin , add cross-linking agent EDMA (0.8 mL) and initiator AIBN (30 mg), degas the above mixture by ultrasonic oscillation for 3~4 min, then pass nitrogen gas for 10 min to evacuate, and seal under nitrogen atmosphere. Thermally initiated polymerization was used to heat it in a constant temperature oil bath at 60 °C for 24 h. After the polymerization was completed, a yellow precipitated polymer was obtained. It was eluted with 10% methanol solution of acetic acid (V / V) to remove the template molecules, and then the precipitate was repeatedly soaked with methanol solution to wash away the residual acetic acid, and dried in vacuum. That is, F-MIPs are obtained. The method for preparing...

Embodiment 2

[0029]Dissolve 0.1 mmol of the template molecule cyhalothrin, 0.0105 g of allyl fluorescein and 0.4 mmol of the functional monomer (AM) in 60 mL of acetonitrile, ultrasonically oscillate, and pre-polymerize at room temperature for 12 hours, so that the functional monomer and cyhalothrin can fully interact , add cross-linking agent EDMA (1.6 mL) and initiator AIBN (60 mg), degas the above mixture by ultrasonic oscillation for 3~4 min, then pass nitrogen gas for 10 min to evacuate, and seal under nitrogen atmosphere. Thermally initiated polymerization was used to heat it in a constant temperature oil bath at 60 °C for 24 h. After the polymerization was completed, a yellow precipitated polymer was obtained. It was eluted with 10% methanol solution of acetic acid (V / V) to remove the template molecules, and then the precipitate was repeatedly soaked with methanol solution to wash away the residual acetic acid, and dried in vacuum. The method for preparing F-NIPs is the same as abov...

Embodiment 3

[0031] First, 5 concentrations of cyhalothrin standard ethanol solutions (0, 0.1, 0.25, 0.5, 1.0 nM) were accurately prepared, 100 mg of fluorescent imprinted polymer was weighed and dispersed in 100 ml of ethanol, and ultrasonically oscillated for 30 minutes. Measure 5 ml of each of the prepared 5 kinds of cyhalothrin solutions, transfer them into 10 ml colorimetric tubes, then add 5 ml fluorescent polymer dispersion into each colorimetric tube, sonicate for 3-5 minutes, and let stand for 2 minutes . The fluorescence intensity of the dispersion in each colorimetric tube was detected by a fluorescence spectrophotometer, and the fluorescence intensity-concentration standard curve was drawn according to the Stern-Volmer equation. fluorescence quenching see Figure 4 .

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Abstract

The invention relates to a method for preparing a molecularly imprinted polymer for fluorescence detection of cyhalothrin. By adopting the method, a spherical fluorescent molecularly imprinted polymer (F-MIP) of taking pyrethroid cyhalothrin as a template molecule, acrylamide (AM) as a functional monomer, allyl fluorescein as fluorochrome, ethylene glycol bi(methacrylic acid) ester (EGDMA) as a crosslinking agent and 2,2'-azobisisobutyronitrile (AIBN) as an initiator is synthesized by adopting a precipitation polymerization method. The prepared fluorescent imprinted polymer has high sensitivity and low detection limit, has strong selective recognition ability on cyhalothrin, and can be applied to quantitative detection analysis of ultratrace cyhalothrin.

Description

technical field [0001] The invention relates to a preparation method suitable for selective recognition and fluorescence detection of cyhalothrin imprinted polymer, which belongs to the technical field of preparation of environmental functional materials. Background technique [0002] Molecular imprinting technology (MIT) is the process of preparing polymers with specific recognition ability for a specific molecule. The prepared polymers are called Molecularly imprinted polymers (MIPs). In recent years, MIPs have attracted more and more scientific workers' interest and favor due to their structure-activity predeterminability, specific recognition and wide practicability. In recent years, many research works have introduced fluorescent dyes into molecular imprinting technology to prepare multifunctional composite fluorescent molecular imprinting materials. The preparation of composite fluorescent molecularly imprinted materials has further expanded the application range and ...

Claims

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Application Information

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IPC IPC(8): C08F220/56C08F222/14C08J9/26C08J9/28B01J20/26B01J20/30
Inventor 高林闫永胜李秀颖潘建明戴江栋卫潇宋志龙
Owner JIANGSU UNIV
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