Method for preparing reducing sugar from material containing cellulose

A technology of cellulose and cellulase, applied in the direction of fermentation, etc., can solve the problem of low yield of reducing sugar and achieve the effect of increasing yield

Active Publication Date: 2014-07-02
COFCO NUTRITION & HEALTH RES INST +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the defect of low production rate of reducing sugar prepared by the existing method, an

Method used

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  • Method for preparing reducing sugar from material containing cellulose
  • Method for preparing reducing sugar from material containing cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This example is used to illustrate the method for preparing reducing sugar from cellulose-containing raw materials provided by the present invention.

[0039] The wheat straw was dried at 100° C. so that the water content therein was 1.5% by weight. And the obtained wheat stalks were crushed into granules with a particle size less than 500 microns, then mixed evenly with a dilute sulfuric acid solution with a concentration of 0.5% by weight in a weight ratio of 4:100, and then in a microwave digestion instrument (purchased from Shanghai Yiyao Instruments) Science and Technology Development Co., Ltd., model WX-8000, the same below) for microwave digestion at 100°C for 30 minutes. Wherein, the microwave frequency used in the microwave digestion is 2.45GHz, and the pressure used is 0.1MPa. The microstructure of the cellulose-containing raw material obtained after microwave digestion is as follows: figure 2 shown. from figure 2 It can be seen that the surface and inte...

Embodiment 2

[0041] This example is used to illustrate the method for preparing reducing sugar from cellulose-containing raw materials provided by the present invention.

[0042] The wheat straw was dried at 105° C. so that the water content therein was 1.8% by weight. And the obtained wheat straw is crushed into powder particles with a particle size of less than 212 microns, and then mixed evenly with a dilute sulfuric acid solution with a concentration of 1% by weight in a weight ratio of 2:100, and then microwaved at 150°C in a microwave digestion apparatus. Digest for 5 minutes. Wherein, the microwave frequency used in the microwave digestion is 2.45GHz, and the pressure used is 0.8MPa. The resulting microwave digestion product was transferred to a hydrolysis vessel and its pH was adjusted to 4 with a 20% by weight sodium hydroxide solution, followed by the addition of 5% by weight of cellulose based on the dry weight of the cellulose-containing raw material used Enzyme (purchased fr...

Embodiment 3

[0044] This example is used to illustrate the method for preparing reducing sugar from cellulose-containing raw materials provided by the present invention.

[0045] The corn stalks were dried at 100° C. so that the water content therein was 1.6% by weight. And the obtained corn stalks are crushed into powder particles with a particle size of less than 270 microns, and then mixed evenly with dilute hydrochloric acid with a concentration of 2% by weight in a weight ratio of 3:100, and then microwave digestion is carried out at 130°C in a microwave digestion apparatus 25 minutes. Wherein, the microwave frequency used in the microwave digestion is 2.45GHz, and the pressure used is 0.2MPa. The resulting microwave digestion product was transferred to a hydrolysis vessel, and its pH was adjusted to 4.8 with a 20% by weight potassium hydroxide solution, followed by the addition of 3% by weight of cellulose based on the dry weight of the cellulose-containing raw material used Enzyme...

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Abstract

The invention provides a method for preparing reducing sugar from a material containing cellulose. The method comprises a step of subjecting the pretreated material containing cellulose and cellulase to mixing and then to hydrolyzation, wherein pretreatment comprises mixing of the material containing cellulose with an acidic solution and microwave digestion at a temperature of 50 to 200 DEG C and a pressure of 0.05 to 2 MPa with 0.5 to 5 GHz microwaves for 1 to 60 min. With the method, the yield of reducing sugar can be increased.

Description

technical field [0001] The invention relates to a method for preparing reducing sugar by using cellulose-containing raw materials. Background technique [0002] At present, the main raw materials for the production of renewable bioenergy such as fuel ethanol, biodiesel, hydrogen and biogas are starchy biomass such as corn and cassava. The use of these raw materials for industrial production will not only cause an unfavorable situation of competing with people for food, but also affect human life. It will have a negative impact on production and production, and will be limited by conditions such as the output, origin and transportation of food resources, making it difficult to meet the growing demand for bioenergy. Cellulose-containing raw materials mainly include crop straws and wild herbs and woody plants, which are one of the most widely distributed and cheapest renewable resources on the earth, and are very suitable as raw materials for the production of renewable bioener...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
Inventor 苏会波李凡彭超林鑫沈乃东林海龙刘文信刘劲松袁敬伟岳国君于天杨
Owner COFCO NUTRITION & HEALTH RES INST
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