Method for preparing pollen nutrient solution by ultrasonic and pulse electric field

A technology of pulsed electric field and high-voltage pulsed electric field, which is applied in food preparation, food ultrasonic treatment, and pharmaceutical formulations. The effect of destruction

Active Publication Date: 2014-07-23
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many problems in the current patent and research of pollen wall breaking. Ultrasonic treatment can well retain the active substances of pollen, but the rate of pollen wall breaking is low, which affects the dissolution of active substances
The effect of single enzymatic treatment is not obvious, the combined enzymatic treatment takes a long time

Method used

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  • Method for preparing pollen nutrient solution by ultrasonic and pulse electric field

Examples

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Effect test

Embodiment 1

[0032] Weigh out 500g of Qinghai-Tibet high crude oil cauliflower pollen. Mix pollen with 1mol / L magnesium sulfate (food grade) solution uniformly to obtain a pollen solution. The mass ratio of pollen: magnesium sulfate solution is 1:20, the mixing time is 10min, the temperature is 60℃, and the stirring speed is 1000r / min. . The pollen solution is pre-treated with ultrasonic (SB-25-12D ultrasonic cleaning machine, Ningbo Xinzhi Biotechnology Co., Ltd.). The ultrasonic treatment conditions are temperature 60℃, ultrasonic frequency 800W, and ultrasonic treatment time 10min. The pollen solution after ultrasonic pretreatment is processed by high-voltage pulsed electric field (South China University of Technology and Food Development), and the pollen solution is transported to the high-voltage pulsed electric field by a liquid pump. The processing conditions are: temperature 60℃, electric field intensity 10kV / cm, The pulse waveform is a square wave, the pulse width is 10 μs, and th...

Embodiment 2

[0034] Weigh 100g of the cleaned Qinghai-Tibet high crude oil rape pollen, mix the pollen with a 0.5mol / L magnesium sulfate (food grade) solution evenly to obtain a pollen solution; wherein the mass ratio of pollen: magnesium sulfate solution is 1:10, and the mixing time 5min, temperature is 40℃, stirring speed is 500r / min. The pollen solution was subjected to ultrasonic pretreatment process (same as Example 1). The ultrasonic treatment conditions were temperature 40°C, ultrasonic frequency 600W, and ultrasonic treatment time 5min. The pollen solution after ultrasonic pretreatment was treated with high-voltage pulsed electric field (same as Example 1). The treatment conditions were: temperature 40°C, electric field intensity 10kV / cm, pulse waveform as square wave, pulse width 5μs, pulse frequency 400Hz, sample The number of receiving electric pulse treatment is 5000, and the above-mentioned high-voltage pulse electric field treatment is cycled twice. The treated pollen solutio...

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Abstract

The invention discloses a method for preparing a pollen nutrient solution by ultrasonic and pulse electric field. The method comprises the following steps: (1) adding the pollen which is free of impurities to a magnesium sulfate solution, and mixing evenly to obtain a pollen solution; (2) performing ultrasonic pretreatment on the pollen solution; and (3) processing the pollen solution processed in step (2) by high-voltage pulsed electric field so as to obtain the pollen nutrient solution. The pollen nutrient solution obtained by the invention has high pollen wall-broken rate and simple process; the damage to the nutrient substance in the pollen is reduced since the extraction process is free of high temperature process.

Description

Technical field [0001] The invention relates to a method for preparing a pollen nutrient solution, in particular to a method for preparing a pollen nutrient solution by ultrasonic and pulsed electric field. Background technique [0002] Bee pollen is a mass formed by processing the pollen from plants that are sourced by bees. Bee pollen is rich in nutrients, not only rich in protein, carbohydrates, lipids, vitamins, organic acids, minerals, and nucleic acids. , Also contains enzymes and other biologically active substances. Bee pollen has certain therapeutic effects on cardiovascular and cerebrovascular diseases, prostate cancer, prostate hyperplasia, liver disease, habitual constipation, anemia, diabetes, and asthma. In addition, it also has anti-fatigue, strengthening physical strength, enhancing memory, improving brain function, enhancing hypoxia tolerance, regulating endocrine, improving sexual function, anti-aging, prolonging life and beauty. However, because pollen has a ...

Claims

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Application Information

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IPC IPC(8): A23L1/076A23L1/025A61K8/97A61Q19/00A23L21/20A23L5/30
CPCA23L5/30A23L5/32A23L21/20A23V2002/00A23V2300/48A23V2300/12
Inventor 曾新安张智宏韩忠王满生刘志伟
Owner SOUTH CHINA UNIV OF TECH
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