Method for preparing pollen nutrient solution by ultrasonic and pulse electric field
A technology of pulsed electric field and high-voltage pulsed electric field, which is applied in food preparation, food ultrasonic treatment, and pharmaceutical formulations. The effect of destruction
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Embodiment 1
[0032] Weigh out 500g of Qinghai-Tibet high crude oil cauliflower pollen. Mix pollen with 1mol / L magnesium sulfate (food grade) solution uniformly to obtain a pollen solution. The mass ratio of pollen: magnesium sulfate solution is 1:20, the mixing time is 10min, the temperature is 60℃, and the stirring speed is 1000r / min. . The pollen solution is pre-treated with ultrasonic (SB-25-12D ultrasonic cleaning machine, Ningbo Xinzhi Biotechnology Co., Ltd.). The ultrasonic treatment conditions are temperature 60℃, ultrasonic frequency 800W, and ultrasonic treatment time 10min. The pollen solution after ultrasonic pretreatment is processed by high-voltage pulsed electric field (South China University of Technology and Food Development), and the pollen solution is transported to the high-voltage pulsed electric field by a liquid pump. The processing conditions are: temperature 60℃, electric field intensity 10kV / cm, The pulse waveform is a square wave, the pulse width is 10 μs, and th...
Embodiment 2
[0034] Weigh 100g of the cleaned Qinghai-Tibet high crude oil rape pollen, mix the pollen with a 0.5mol / L magnesium sulfate (food grade) solution evenly to obtain a pollen solution; wherein the mass ratio of pollen: magnesium sulfate solution is 1:10, and the mixing time 5min, temperature is 40℃, stirring speed is 500r / min. The pollen solution was subjected to ultrasonic pretreatment process (same as Example 1). The ultrasonic treatment conditions were temperature 40°C, ultrasonic frequency 600W, and ultrasonic treatment time 5min. The pollen solution after ultrasonic pretreatment was treated with high-voltage pulsed electric field (same as Example 1). The treatment conditions were: temperature 40°C, electric field intensity 10kV / cm, pulse waveform as square wave, pulse width 5μs, pulse frequency 400Hz, sample The number of receiving electric pulse treatment is 5000, and the above-mentioned high-voltage pulse electric field treatment is cycled twice. The treated pollen solutio...
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