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A freshwater alga chlorella sorokiniana HN01 and its application

A technology of CCTCCM2013608 and algae strains, applied in the field of biomass energy, can solve problems such as difficult large-scale high-density cultivation, high energy consumption costs in the processing process, and water source pollution, so as to ensure high-density, high-quality fermentation and enhance international competitiveness. Effect

Inactive Publication Date: 2016-07-06
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the production process of domestic chlorella is relatively open, and it is difficult to ensure the consistency of batches and the pollution of foreign organisms and water sources. In addition, it is difficult to achieve large-scale high-density cultivation of traditional chlorella cultivation as autotrophic cultivation. High energy consumption and high cost

Method used

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  • A freshwater alga chlorella sorokiniana HN01 and its application
  • A freshwater alga chlorella sorokiniana HN01 and its application
  • A freshwater alga chlorella sorokiniana HN01 and its application

Examples

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Effect test

Embodiment 1

[0059] Under aseptic conditions, single algae were picked from a solid plate and dropped into a 100ml Erlenmeyer flask containing 30ml of sterile BG-11 culture solution, and cultured in a light culture rack. The temperature was 28°C, the light intensity was 7500lux, and the light-to-dark ratio was 12: 12. The culture time is 12 days, and the microalgae seed liquid is obtained by growing to the end of the index.

[0060] 500 mL of BG-11 medium was prepared, and the inoculum of 15 v / v % was inserted into the expanded cultured microalgae seed solution. The initial pH is 4.0, the temperature is 28°C, the light-dark ratio is 12:12 (day: night), the light intensity is 15000lux, the amount of glucose added is 2g / L, and the sterile air of 0.2vvm is passed through, and cultivated for 14 days.

[0061] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of the alg...

Embodiment 2

[0063] Under sterile conditions, single algae were picked from a solid plate and dropped into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and cultured in a light culture rack. The temperature was 28°C, the light intensity was 7500lux, and the light-dark ratio was 12:12. The time is 12 days, and the seed solution is obtained after growing to the end of the index.

[0064] 500 mL of BG-11 medium was prepared, and the inoculum of 5v / v% was inserted into the expanded cell culture medium. The initial pH is 8.0, the temperature is 28°C, the light-dark ratio is 12:12 (day: night), the light intensity is 7500lux, the amount of glucose added is 15g / L, and 0.33vvm sterile air (CO 2 The content is 0.03% (v / v), cultured for 9 days.

[0065] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of the algal powder was 6.2g / L, the biomass y...

Embodiment 3

[0067] Under sterile conditions, single algae were picked from a solid plate and dropped into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and cultured in a light culture rack. The temperature was 28°C, the light intensity was 7500lux, and the light-dark ratio was 12:12. The time is 12 days, grow to the end of the index and expand the culture with a 1:10 inoculation ratio.

[0068] Prepare 500mL of BG-11 medium, and insert the expanded microalgae cells. The initial pH is 11.0, the temperature is 45°C, the light-to-dark ratio is 12:12 (day:night), the light intensity is 2500lux, the amount of glucose added is 15g / L, and 0.33vvm sterile air (CO 2 The content is 0.03% (v / v), cultured for 5 days.

[0069] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of the algal powder was 4.4g / L, the biomass yield was 0.49g / L / d, and the o...

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Abstract

The invention relates to the technical field of biomass energy, and particularly relates to an imnetic chlorella strain Chlorella sorokiniana HN01 and application thereof to high density fermentation for production of chlorella nutritional health products. The imnetic chlorella strain Chlorella sorokiniana HN01 provided by the invention has a preservation number of CCTCC M2013608, can realize high-density high-quality fermentation in a photoheterotrophy-chemoheterotrophy light switching cultivation mode. Chlorella fermented algae powder has biomass of 6. 2g / L, protein content of 39%, and content of essential fatty acid including linoleic acid and linolenic accounting for more than 20% of total fatty acid content. The invention provides a good algae strain and key fermentation technology for wide application of chlorella in the industries of food, nutritional health products, cosmetics medicine and chemical engineering, and can ensure high-density high-quality fermentation of chlorella and enhance the international competitiveness of China's chlorella industry.

Description

technical field [0001] The invention relates to the technical field of biomass energy, in particular to a freshwater algal strain Chlorellasorokiniana HN01 and its application in the production of chlorella nutrition and health products by a high-density fermentation method. Background technique [0002] Algae are the oldest of all plants. According to the color of the pigment, it can be divided into three categories: green algae, brown algae and red algae; according to different ecological habits, algae can be divided into planktonic algae and benthic algae; algae can be divided into freshwater algae, seaweed, and salt water according to the growth area. Algae, flowing algae, aerial algae, soil algae, hot spring algae, ice and snow algae, and epiphytic algae; Chinese scholars generally divide algae into 11 categories: cyanobacteria, red algae, cryptophytes, dinoflagellates, golden algae, and yellow algae , diatoms, brown algae, euglena, green algae, chara. [0003] As an ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/62C12P7/64C12R1/89
Inventor 缪晓玲巩三强
Owner SHANGHAI JIAOTONG UNIV
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