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A kind of cadmium-tolerant bacteria and method for inhibiting rice to absorb heavy metal cadmium

A technology of bacterial inhibition and heavy metals, applied in the fields of agriculture and environmental pollution control, can solve the problems of soil structure damage, content reduction, complex equipment, etc., and achieve the effects of reducing cadmium content in brown rice, no inhibition of seedling growth, and strong repair effect.

Inactive Publication Date: 2016-02-24
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many remediation methods for soil heavy metal pollution. The commonly used physical and physicochemical remediation methods are generally expensive in investment, complicated in equipment and easy to cause damage to soil structure. However, some microorganisms have high resistance to heavy metals and strong adsorption capacity. Using these microorganisms to absorb and fix farmland Heavy metals in the soil can inhibit the absorption of heavy metals by crops, reduce their content in crops, and ensure the safety of agricultural products

Method used

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  • A kind of cadmium-tolerant bacteria and method for inhibiting rice to absorb heavy metal cadmium
  • A kind of cadmium-tolerant bacteria and method for inhibiting rice to absorb heavy metal cadmium
  • A kind of cadmium-tolerant bacteria and method for inhibiting rice to absorb heavy metal cadmium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Screening of bacterial strain TCd-1

[0018] Dissolve 5 g of rice rhizosphere soil in 50 mL of sterile water, and take 150 μL of the solution in sterilized beef extract-peptone liquid medium (medium: beef extract 3 g L -1 , peptone 10g·L -1 , NaCl5g L -1 , agar 20g·L -1 , pH7.3), on a shaker at 150rpm, shake at 37°C for 12h. Then draw 0.1mL from it and spread it on the -1 Wait for 5 beef extract peptone solid medium plates with different cadmium concentrations, set 3 replicates in each group, and culture them upside down in a 37°C incubator for 24h. According to the growth of bacteria, take 300mg·L -1 Streak culture was carried out on the plate medium with cadmium concentration, and the operation was repeated more than 3 times to observe the growth of the colony TCd-1, and the slant was stored in a refrigerator at 4°C until use.

Embodiment 2

[0019] Example 2: Cadmium uptake properties of bacterial strain TCd-1

[0020] Under sterile conditions, the strain TCd-1 preserved in Example 1 was transferred to beef extract peptone liquid medium, and cultured with shaking at 150 rpm at 37° C. for 12 hours was used as a seed solution. Draw 1mL of TCd-1 seed solution into 100mL cadmium concentration is 100mg·kg -1 The beef extract peptone liquid medium was cultured with shaking at 37°C for 24h. Take 10mL of the culture solution, centrifuge at 10000rpm for 10min, rinse the cell pellet with sterile water for 3 times, dry and weigh it, and use it to determine the cadmium content of the cell. After the same treatment as above, the bacterial cell sediment was suspended in 10 mL of sterile water, ultrasonically broken for 140 s, and centrifuged at 12,000 rpm for 10 min to obtain the cell wall precipitate, which was used for the determination of cadmium content in the cell wall of the bacterial cell, and the supernatant was used...

Embodiment 3

[0023] Example 3: Restoration of rice growth under cadmium stress by bacterial strain TCd-1

[0024] Take the germinated rice seeds, and implant them evenly containing 5 mg·L -1 CdCl 2 10 seeds were implanted in each petri dish, and the agarose medium implanted with no cadmium treatment was used as a control, and 5ml of distilled water (treatment Ⅰ) and bacterial solution of strain TCd-1 (treatment Ⅱ) Two treatments, each treatment repeated 5 times. Each treatment was placed in a greenhouse at 25°C for light cultivation. Water was supplemented quantitatively every day, and rice biomass was measured after 7 days. The results showed that under cadmium stress, the biomass of rice treated with TCd-1 bacterial solution was similar to that of the control. The plant height and fresh weight of rice treated with liquid solution increased by 38.4% and 38.1%, respectively, and the restoration effect was extremely significant. (As shown in table 2)

[0025] Table 2 Restoration e...

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Abstract

The invention relates to a heavy metal cadmium-tolerant bacterium and a method for inhibiting crops from absorbing heavy metal cadmium, and belongs to the technical field of agriculture and environmental pollution control. Described bacterial strain is Pseudomonas ( Pseudomonas? sp . ) TCd-1, which was registered and preserved in the Chinese Type Culture Collection Center of Wuhan University on December 11, 2013, with the strain preservation number CCTCC? M? 2013649. Strain TCd-1 was identified as Pseudomonas ( Pseudomonas? sp . ) bacteria, which are resistant to a variety of heavy metals, and the resistant concentrations to cadmium, lead and nickel respectively reach 900? mg·L-1, 800? mg·L-1? and 600? mg·L-1. The effective number of viable bacteria in the bacterial liquid is about 1.85~1.87×109 / ml. Add this bacterial solution to the wet soil polluted by heavy metal cadmium, and add 2? mL, can repair the inhibitory effect of cadmium on rice growth, and effectively reduce the cadmium content in various parts of rice, especially brown rice.

Description

technical field [0001] The invention relates to a cadmium-tolerant bacterium and a method for inhibiting rice from absorbing heavy metal cadmium, and belongs to the technical field of agriculture and environmental pollution control. technical background [0002] Cadmium is a highly toxic heavy metal element. Cadmium in the soil is easily enriched by crops and endangers human health through the food chain. At present, the area of ​​cultivated land polluted by toxic heavy metals in my country has reached 20 million hm 2 , accounting for about 1 / 5 of the total cultivated land area. Therefore, the goal of controlling heavy metal pollution in farmland is not only to suppress the toxicity of heavy metals to crops and increase their yield, but more importantly, to reduce the absorption of heavy metals by crops and inhibit their entry into the food chain. There are many remediation methods for soil heavy metal pollution. Currently, the commonly used physical and physicochemical rem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/38
Inventor 林瑞余肖清铁周丽英谢惠玲李艺林文雄
Owner FUJIAN AGRI & FORESTRY UNIV
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