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Method for crosslinking oil-soluble epoxy compound and collagen in supercritical carbon dioxide

A technology of epoxy compound and carbon dioxide, which is applied in the production of animal fiber, bulk chemicals, small raw hide/large raw hide/leather/fur treatment, etc. It can solve solvent residues, difficult organic solvents, and unfavorable biocompatibility of cross-linked materials Impact and other issues

Active Publication Date: 2014-08-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the organic solvent is difficult to completely remove, there may be solvent residues, which will adversely affect the biocompatibility of the cross-linked material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Weigh 10 grams of acellular porcine dermal matrix (dry weight);

[0033] (2) Put it in 100ml pH10.4 sodium hydroxide-sodium carbonate buffer solution, soak for 30min, freeze-dry for later use;

[0034] (3) Weigh 0.8 g of pentaerythritol tetraglycidyl ether, add it to a supercritical carbon dioxide reactor, then put the above-mentioned treated acellular porcine dermal matrix into the reactor, and close the reactor;

[0035] (4) Turn on the temperature control switch and adjust the reactor temperature to 40°C;

[0036] (5) Turn on the pressurization switch, add carbon dioxide, pressurize to 9.5Mpa, and turn off the pressurization switch;

[0037] (6) Turn on the stirring device, and react for 48 hours at the aforementioned temperature and pressure;

[0038] (7) Turn off the heating switch, open the carbon dioxide recovery valve, and recover CO 2 gas;

[0039] (8) Open the reactor, add 0.2 g of RPMI-1640 dry powder medium, turn on the stirring device, adjust the te...

Embodiment 2

[0044] (1) Weigh 100 grams of collagen sponge and put it into a supercritical carbon dioxide reactor;

[0045] (2) Weigh 4 grams of ethylene glycol diglycidyl ether into the reactor, and close the reactor;

[0046] (3) Turn on the stirring device, adjust the temperature of the reactor to 37°C, inject carbon dioxide gas to make the pressure 8.0Mpa, and react for 12 hours;

[0047] (4) Stop stirring, turn off the temperature control switch, open the recovery valve, and recover CO 2 gas;

[0048] (5) Open the reactor, weigh 0.5 g of RGD and add it to the reactor, adjust the temperature of the reactor to 37°C, and pass in CO 2 Gas, pressurized to 8.0Mpa, reacted for 12 h;

[0049] (6) Turn on the stirring device, adjust the temperature to 40°C, add carbon dioxide, pressurize to 9.5MPa, and extract for 8 hours;

[0050] (7) Turn off the heating switch, open the carbon dioxide recovery valve, and recover CO 2 Gas, open the reactor, and take out the cross-linked collagen sponge....

Embodiment 3

[0052] (1) Weigh 1 gram of collagen-based micro-nano fibers and place them in a supercritical carbon dioxide reactor;

[0053] (2) Add 0.1 g of glycerol triglycidyl ether to the reactor to close the reactor;

[0054] (3) Turn on the stirring device, adjust the temperature of the reactor to 35°C, inject carbon dioxide gas to make the pressure 8.5MPa, and react for 24 hours;

[0055] (4) Stop stirring and discharge CO 2 gas;

[0056] (5) Open the reactor, add 0.3 g of lysine, adjust the temperature to 37°C, the pressure to 8.5Mpa, and react for 12 hours;

[0057] (6) Introduce CO into the reactor 2 Gas, adjust the temperature to 37°C, the pressure to 8.5MPa, keep the gas for 8 hours and then discharge the gas;

[0058] (7) Stop stirring, turn off the temperature controller, recover carbon dioxide, open the reactor, and take out the cross-linked collagen-based fibers.

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PUM

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Abstract

The invention relates to a method for crosslinking an oil-soluble epoxy compound and collagen in supercritical carbon dioxide. The method is characterized in that the supercritical carbon dioxide is taken as a medium, the oil-soluble epoxy compound and the collagen have a crosslinking reaction; after crosslinking is ended, an active substance is added, unreacted epoxy groups are closed and the active substance is grafted to the collagen, finally, the supercritical carbon dioxide is taken as an extraction agent to remove impurities. The method can be used in medical biological materials and leather industry.

Description

technical field [0001] The invention relates to a method for cross-linking collagen with an oil-soluble epoxy compound in a supercritical carbon dioxide medium, which can be applied to biomedical materials and leather industries. Background technique [0002] Collagen is the main structural protein of connective tissue, accounting for about 1 / 3 of the total protein in mammals, and widely exists in animal skin, bone, cartilage, tendon, ligament and blood vessel wall, etc., in the form of three-dimensional network of collagen fibers exist. Collagen has many advantages such as low immunogenicity, low irritation, low cytotoxicity, good biocompatibility, good performance of promoting cell growth and biodegradability, etc., and it is widely used in the field of biomedicine. However, uncrosslinked collagen degrades too quickly in vivo to match the growth of the target tissue. Cross-linking collagen by physical and chemical methods is beneficial to reduce antigenicity, improve phy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00C08K5/1515C14C3/08C08J9/40D06M13/11D06M101/14
CPCY02P20/54
Inventor 但年华但卫华陈一宁
Owner SICHUAN UNIV