Method for preparing noble metal palladium nanorods by utilizing the nucleocapsid of cotton bollworm baculovirus
A technology of baculovirus and cotton bollworm is applied in the field of preparation of nanomaterials, which can solve the problems of unsatisfactory morphology and dimensional stability, different morphology of palladium nanorods, and increase the complexity of the experimental process. The effect of uniformity, easy availability of raw materials and low production cost
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Embodiment 1
[0019] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by water meter, obtain off-white polyhedron suspension, add alkaline hydrolysis solution 1.25mL to the polyhedron suspension and let stand for 15min, the alkaline hydrolysis solution is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and a mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1.25 mL of chloroform, and shake for 5 min. After centrifuging at 5000r / min for 10min, suck out the upper layer liquid, that is, the supernatant, put each 4mL supernatant into an ultracentrifuge tube, centrifuge at 28000r / min at 4°C for 1h, and reconstitute the precipitated spots with 1.33mL PBS solution. Suspended to obtain the cotton bollworm baculovirus nucleocapsid solution.
[0020] Such as figure 1 As shown, it can be seen that the template used is a rod-shaped structure. The nucleocapsid of the cotton bollwo...
Embodiment 2
[0024] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by water meter, obtain off-white polyhedron suspension; Add alkaline hydrolyzate 0.83mL to polyhedron suspension and leave standstill 25min, described alkaline hydrolyze is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and A mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1.46 mL of chloroform, and shake for 8 min. After centrifuging at 6000r / min for 5min, suck out the supernatant, the upper layer liquid, put each 6mL supernatant into an ultracentrifuge tube, centrifuge at 10°C and 30000r / min for 2h, and wash each precipitated spot with 1.2mL PBS The solution is resuspended to obtain the nucleocapsid solution of the cotton bollworm baculovirus.
[0025] Such as figure 2 As shown, it can be seen that the baculovirus nucleocapsid is composed of multiple helical capsid particles. The essence of th...
Embodiment 3
[0029] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by a water meter, obtain off-white polyhedron suspension, add 1mL alkaline hydrolysis solution to the polyhedron suspension and let stand for 20min, the alkaline hydrolysis solution is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and A mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1 mL of chloroform, and shake for 10 min. After centrifuging at 5500r / min for 10min, suck out the upper layer liquid, i.e. the supernatant, put each 5mL supernatant into an ultracentrifuge tube, centrifuge at 25000r / min at 6°C for 1.5h, and use 1.25 mLPBS solution is resuspended to obtain the cotton bollworm baculovirus nucleocapsid solution.
[0030] Take 300 μL of the baculovirus nucleocapsid solution of cotton bollworm and add 75 μL of PdCl with a concentration of 10 mM 2 The solution was thoroughly mixed and inc...
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