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Antigen-free collagen aggregates and preparation method thereof

An aggregate and antigen-free technology, applied in prosthetics, medical science, etc., can solve the problems of poor biodegradability, poor mechanical properties, and low structural stability, and achieve low cost, excellent mechanical properties, and favorable adhesion. Effect

Active Publication Date: 2016-05-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The application of collagen in medical biomaterials still has problems such as poor mechanical properties, poor biodegradability, and low structural stability.

Method used

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  • Antigen-free collagen aggregates and preparation method thereof
  • Antigen-free collagen aggregates and preparation method thereof
  • Antigen-free collagen aggregates and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A) Bovine Achilles tendon pretreatment: take fresh and traceable bovine Achilles tendon, remove the muscle, marginal cartilage, blood vessels and fascia tissue on the surface of the bovine Achilles tendon, then cut it into 1.0mm×1.0mm slices, and use Soak in normal saline for 30 minutes, then rinse repeatedly with double distilled water;

[0035] B) Degreasing treatment of bovine Achilles tendon: put 10 parts by weight of bovine Achilles tendon slices in a supercritical fluid processor, add 200 parts by volume of acetone, and treat for 1 hour at a pressure of 10.0Mpa and room temperature. Rinse repeatedly with double distilled water until the beef Achilles tendon has no smell of acetone;

[0036] C) Bovine Achilles tendon removal of non-collagen components, decellularization: Under ultrasonic conditions, soak the degreased animal skin or Achilles tendon in Tris-NaCl (Tris: 0.05mol / L; NaCl: 1mol / L; pH7. 5) In the buffer solution, stir at room temperature for 10 hours, t...

Embodiment 2

[0039] A) Pigskin pretreatment: take fresh and traceable pigskin, remove the muscle, marginal cartilage, blood vessels and fascia tissue of the pigskin, then cut it into thin slices of 3.0mm×3.0mm, soak it in normal saline for 60min at room temperature , and then rinsed repeatedly with double distilled water;

[0040] B) Degreasing treatment of pigskin: put 10 parts by weight of pigskin thin slices in a supercritical fluid processor, add 300 parts by volume of acetone, and treat for 3 hours at a pressure of 20.0Mpa and room temperature. Rinse with water repeatedly until the pigskin has no smell of acetone;

[0041] C) Pigskin removes non-collagen components and decellularizes: Under ultrasonic conditions, soak the degreased pigskin in Tris-NaCl (Tris: 0.05mol / L; NaCl: 1mol / L; pH7.5) buffer solution , stirred at room temperature for 15 hours, and then placed in a -80°C freezer for 5 times of freezing and thawing, and washed repeatedly with double distilled water; continued to so...

Embodiment 3

[0044] A) Porcine Achilles tendon pretreatment: Take fresh and traceable porcine Achilles tendon, remove the muscle, marginal cartilage, blood vessels and fascia tissue on the surface of the porcine Achilles tendon, then cut it into 5.0mm×5.0mm slices, and use it at room temperature. Soak in normal saline for 90 minutes, then rinse repeatedly with double distilled water;

[0045] B) Pig Achilles tendon degreasing treatment: put 10 parts by weight of thin slices of pig Achilles tendon in a supercritical fluid processor, add 400 parts by volume of acetone, and treat it for 5 hours at a pressure of 35.0Mpa and room temperature. Rinse repeatedly with double distilled water until the pig Achilles tendon has no smell of acetone;

[0046] C) Porcine Achilles tendon removal of non-collagen components, decellularization treatment: Under ultrasonic conditions, soak the degreased pig Achilles tendon in Tris-NaCl (Tris: 0.05mol / L; NaCl: 1mol / L; pH7.5) In the buffer solution, stir at room...

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Abstract

The invention discloses an antigen-free collagen aggregate and a preparation method thereof. The preparation method is characterized by comprising the following steps: with traceable animal skin or tendo calcaneus as a raw material, performing operations by the process such as fleshing, stripping fascia, degreasing, removing foreign protein and decellularizing; performing fine purification on the animal skin or tendo calcaneus, and then carrying out separation and purification on the collagen aggregate by the methods of acid fluffing, homogenizing, salting out for a plurality of times, centrifuging for a plurality of times and the like, thereby finally obtaining the antigen-free collagen aggregate. The collagen aggregate is a mixture of a collagen fiber and a collagen bundle, and has a periodic light and shade horizontal grain structure; and the space between the horizontal grains is about 67nm. The material has good biocompatibility, biodegradability, mechanical property and hemostatic performance, also has the characteristics of low antigenicity, biological activity and the like, and can be widely applied to preparation of biomedical materials such as a hemostatic material, a tissue engineering material, a biological dressing, a biodegradable medical suture and a plastic material.

Description

technical field [0001] The invention relates to an antigen-free collagen aggregate and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] Collagen is the main skeleton component of extracellular matrix, widely exists in skin, bone, tendon, etc., and has supporting and protecting functions. Collagen aggregates are aggregates of collagen molecules arranged in bundles and interwoven with each other in connective tissue. Collagen molecules are covalently cross-linked laterally, arranged in a stepwise order, and aggregated into fibrils with a diameter of 50-200 nm and a length of 280 nm to several microns. These fibrils form collagen fibers, and collagen fibers aggregate to form collagen fiber bundles. At present, the spatial arrangement of collagen molecules is the microfibril model proposed by Smith (Zhan Huaiyu, Fiber Chemistry and Physics [M]. Science Press, 2005: 383). According to this model, the cros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/58A61L27/50
Inventor 但卫华刘新华但年华薛媛刘科
Owner SICHUAN UNIV
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