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A kind of method for preparing xylo-oligosaccharide

A xylo-oligosaccharide and lignocellulose technology, which is applied in the production of sugar, sugar production, food science and other directions, can solve the problems of high concentration of by-products, low yield of xylo-oligosaccharide, etc. The effect of reducing processing time and avoiding waste of resources

Active Publication Date: 2016-03-30
黑龙江阳光惠远知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the dense structure of hemicellulose, the acid hydrolysis treatment time is too long, which leads to problems such as high concentration of by-products (such as furfural) and low yield of xylo-oligosaccharides.

Method used

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  • A kind of method for preparing xylo-oligosaccharide
  • A kind of method for preparing xylo-oligosaccharide
  • A kind of method for preparing xylo-oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 hardwood chip source xylo-oligosaccharide

[0046] 1. Preparation of lignocellulosic raw materials

[0047] Lignocellulosic raw materials were formulated according to the following formula:

[0048] Hardwood chips 92% (dry weight, w / w), corn flour 6%, gypsum 1.5%, superphosphate 0.5%.

[0049] 2. Fungal fermentation treatment

[0050] Pleurotus eryngii Riyin No. 1 purchased from Zhangzhou Academy of Agricultural Sciences was used as the fermentation strain. A cultivation bag with a caliber of 17.5 cm is used, and the charging amount is 1200 g of wet material, and the water content is 65%. Lignocellulosic raw materials are filled according to the formula, then put on a plastic ring, plugged with a cotton plug, and then sterilized by autoclaving at 121°C for 2.5h, cooled to below 30°C for inoculation, the inoculum amount is 2% (w / w ). The inoculated culture bag was transferred to the culture room, protected from light, at 23°C, humidit...

Embodiment 2

[0057] The preparation of embodiment 2 corncob source xylo-oligosaccharides

[0058] 1. Preparation of lignocellulosic raw materials

[0059] Lignocellulosic raw materials were formulated according to the following formula:

[0060] Corncob 92% (dry weight, w / w), corn flour 6%, gypsum 1.5%, superphosphate 0.5%.

[0061] 2. Fungal fermentation treatment

[0062] Pleurotus eryngii Riyin No. 1 purchased from Zhangzhou Academy of Agricultural Sciences was used as the fermentation strain. A cultivation bag with a caliber of 17.5 cm is used, and the charging amount is 1200 g of wet material, and the water content is 65%. Lignocellulosic raw materials are filled according to the formula, then put on a plastic ring, plugged with a cotton plug, and then sterilized by autoclaving at 121°C for 2.5h, cooled to below 30°C for inoculation, the inoculum amount is 2% (w / w ). The inoculated culture bag was transferred to the culture room, protected from light, at 23°C, humidity 75%, CO 2...

Embodiment 3

[0070] 1. Preparation of lignocellulosic raw materials

[0071] Lignocellulosic raw materials were formulated according to the following formula:

[0072] Corn stalks 60% (dry weight, w / w), corn flour 38%, gypsum 1.5%, superphosphate 0.5%.

[0073] 2. Fungal fermentation treatment

[0074] Pleurotus eryngii Riyin No. 1 purchased from Zhangzhou Academy of Agricultural Sciences was used as the fermentation strain. A cultivation bag with a caliber of 17.5 cm is used, and the charging amount is 1200 g of wet material, and the water content is 65%. Lignocellulosic raw materials are filled according to the formula, then put on a plastic ring, plugged with a cotton plug, and then sterilized by autoclaving at 121°C for 2.5h, cooled to below 30°C for inoculation, the inoculum amount is 2% (w / w ). The inoculated culture bag was transferred to the culture room, protected from light, at 23°C, humidity 75%, CO 2 Bacteria were produced under the condition of concentration 2000-2500μL / L...

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Abstract

The invention discloses a preparation method for xylo-oligosaccharide, which belongs to the technical field of biochemical industry. The method comprises the following steps: with lignocellulose as a raw material, carrying out fermentation pretreatment with edible fungi so as to obtain a fermentation product; subjecting the fermentation product to acid hydrolysis so as to prepare xylo-oligosaccharide; and extracting xylo-oligosaccharide from hydrolysate. Compared with traditional acid hydrolysis methods, the method provided by the invention has the advantages of high yield of xylo-oligosaccharide, low reaction temperature, short reaction time, etc., wherein the yield of xylo-oligosaccharide can be increased by 64.8%, hydrolysis temperature is decreased by 10 DEG C, and hydrolysis time is shortened by more than 30 min. According to the invention, fungus fermentation slag is effectively utilized, so the environment is protected, waste of resources is avoided, and the added value of the product is increased; thus, the method has immense application potential and a great popularization value.

Description

technical field [0001] The invention relates to a method for preparing xylooligosaccharides, which belongs to the technical field of biochemical industry. Background technique [0002] Xylo-oligosaccharides (Xylo-oligosaccharides, XOS) is a non-digestible polysaccharide obtained by chemical or enzymatic hydrolysis of xylo-polysaccharides in plant cell walls, which are linked by β-1,4-glycosidic bonds by 2-7 xylose In addition, it also contains arabinuronic acid and glucuronic acid side chains. Because xylooligosaccharide has physiological functions such as regulating intestinal function, low calorie, preventing dental caries, promoting calcium absorption, non-toxic safety, anti-inflammation, anti-fatigue, lowering blood pressure, blood sugar, blood lipid and cholesterol, etc., it has become a domestic product in recent years. foreign research hotspots. Xylo-oligosaccharides are non-digestible carbohydrates that can confer health benefits on the host through the adjustment ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C13K13/00
Inventor 张东旭
Owner 黑龙江阳光惠远知识产权运营有限公司