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A kind of preparation method of hyaluronic acid/bacterial cellulose composite hydrogel

A bacterial cellulose and composite hydrogel technology, applied in the biological field, can solve the problems of difficult water recovery, easy water extrusion, low compressive modulus, etc., and achieves difficult to be contaminated by microorganisms, good light transmittance, and low production cost. Effect

Inactive Publication Date: 2018-06-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the bacterial cellulose membrane has a high tensile strength, its compression modulus is low, and the moisture is easy to squeeze out after being compressed, and the lost moisture is difficult to recover; moreover, the bacterial cellulose wet membrane must be stored at low temperature, and it needs to be considered prevent microbial contamination

Method used

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  • A kind of preparation method of hyaluronic acid/bacterial cellulose composite hydrogel
  • A kind of preparation method of hyaluronic acid/bacterial cellulose composite hydrogel
  • A kind of preparation method of hyaluronic acid/bacterial cellulose composite hydrogel

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Effect test

Embodiment 1

[0046] A preparation method of hyaluronic acid / bacterial cellulose composite hydrogel, the steps are as follows:

[0047] ⑴Put the slanted seeds of Acetobacter xylinum CGMCC No.2955 (stored in China General Microorganism Culture Collection and Management Center) into a conical flask containing 100mL seed medium, and vibrate at 30°C for 24 hours to obtain Acetobacter xylinum seeds . Acetobacter xylinum seed is inserted in the fermentation medium with 5% inoculum size (the fermentation medium is prepared like this: 1L water contains glucose 20g, yeast extract 5g, peptone 5g, Na2HPO42.7g, citric acid 1.15g, pH6 .0). After 6 days, the inoculation amount was 5% (V / V), and it was inserted into a container containing HS medium, and the bacterial cellulose membrane was taken out after 6 days.

[0048] (2) Wash off the medium and impurities on the surface of the membrane with distilled water, then soak the membrane in 0.1M NaOH solution, boil for 30 minutes, further remove a large am...

Embodiment 2

[0054] A preparation method of hyaluronic acid / bacterial cellulose composite hydrogel, the steps are as follows:

[0055] (1) During fermentation, the seeds of Acetobacter xylinum were inserted into the HS medium with an inoculation amount of 10%.

[0056] (2) When preparing the gel, make the mass concentration of hyaluronic acid in 0.24% (g / mL) BC suspension reach 40g / L. The water content of the gel exceeds 90%. The gel is elastic in a certain range of shear rate by Haake rheometer (MarsII).

[0057] (3) After it is dried, it is set aside to obtain a "dried gel".

[0058] Soak the "dried gel" in distilled water until it no longer swells, and take it out from the distilled water. The light transmittance of the "rehydrated gel" is 91%. After swelling in water, it absorbs water and becomes its own dry weight 29.9 times. The dry gel film with a thickness of 20 μm becomes 1.2 cm after fully swelling.

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Abstract

The invention relates to a preparation method of a hyaluronic acid / bacterial cellulose composite hydrogel, which comprises taking out the bacterial cellulose membrane after bacterial fermentation, crushing the bacterial cellulose membrane, and homogenizing under high pressure to obtain the bacterial cellulose suspension , dissolving hyaluronic acid therein, adjusting the pH value to be acidic, and continuously stirring to obtain an acidic gel, then washing the gel until it is neutral, and drying it in vacuum to obtain a hyaluronic acid bacterial cellulose composite dry gel. The gel prepared by this method has a three-dimensional network structure, and the material has good light transmittance. It can be seen from Figure 2 that the dried gel retains the good light transmittance and high water content of the hyaluronic acid / bacterial cellulose composite hydrogel after rehydration , it can be seen from Figure 3 that its morphology is a typical gel feature, and the average pore size is tens of microns. It can be seen from Figure 4 that at a shear rate of 0.1-100Hz, this material behaves as an elastic body, which proves that it is here The scope has good elastic properties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of hyaluronic acid / bacterial cellulose composite hydrogel. technical background [0002] In recent years, due to energy shortage and demand for environmentally friendly materials, cellulose, as a rich and renewable natural resource, has received more and more attention in the field of development and utilization of natural renewable resources. Bacterial cellulose (BC) is cellulose synthesized by certain bacteria. Although BC and plant-produced cellulose have the same molecular structure unit, unlike plant cellulose, BC has no associated products such as lignin, pectin and hemicellulose, and has high chemical purity, high crystallinity and high degree of polymerization. . It also has an ultra-fine network structure, high elastic modulus, and strong water holding capacity. BC is easy to regulate during biosynthesis. Its excellent biocompatibility, adaptability an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L1/02C08L5/08A61L27/40A61L27/52A61L27/56A61K9/06A61K47/36A61K47/38
Inventor 贾原媛王艳吴智敏常冬梅霍明明王曼
Owner TIANJIN UNIV OF SCI & TECH
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