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Biological extraction method of high-unsaturated-activity salicornia bigelovii seed oil

A technology of biological extraction and Salicornia, applied in the field of biological extraction of highly unsaturated active Salicornia clear oil, can solve the problems of inflammability and explosion, deep color, large energy consumption, etc. Simple equipment and good production safety

Inactive Publication Date: 2014-11-19
NANJING FEIMA FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pressing method mainly uses mechanical external force to squeeze the oil out of the oil. The product does not contain solvent, but the cake has a lot of residual oil, the oil output efficiency is low, and the energy consumption is large; the solvent method has a high oil output rate and is easy to realize large-scale production. However, most of the solvents used are flammable and explosive, and the production safety is poor, and the refined oil contains solvent residues, and the leached crude oil contains more non-oil substances, darker in color, and poor in quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1kg of Salicornia seed and mix it with water at a ratio of 1:5, and grind it with a JMS-50 colloid mill (manufactured by Langfang Guantong Machinery Co., Ltd.) until the particle size is 2-50 μm to obtain the salicornia slurry; in the slurry Add compound wall-breaking enzymes (4g pectinase NCB-PE40, 6g cellulase AE80), enzymatically hydrolyze for 3h at 50°C and pH4.5, then adjust the pH to about 9, raise the temperature to 60°C, keep for 40min, add 2.5 ml alkaline protease Alcalase 2.4L, keep at 60°C and pH value of about 9, enzymatic hydrolysis for 60min, centrifuge at 50°C and 3500rpm for 10min, take the supernatant to obtain samphire clear oil, finally add 0.4g linoleic acid Isomerase converts linoleic acid in salicornia clear oil into conjugated linoleic acid to obtain highly unsaturated active salicornia clear oil.

Embodiment 2

[0021] Take 1kg of Salicornia seed and mix it with water at a ratio of 1:5, and grind it with a JMS-50 colloid mill (manufactured by Langfang Guantong Machinery Co., Ltd.) to a salicornia slurry with a particle size of 2-50 μm; add it to the slurry Add compound wall-breaking enzymes (4g pectinase NCB-PE40, 6g cellulase AE80), enzymatically hydrolyze for 3h at 50°C and pH4.5, then adjust the pH to about 9, raise the temperature to 60°C, keep for 40min, add 2.5 ml alkaline protease Alcalase 2.4L, keep 60°C and pH value of about 9, enzymatic hydrolysis for 60min, centrifuge at 50°C and 3500rpm for 10min, take the supernatant to obtain Salicornia saponica clear oil, finally add 0.6g linoleic acid Isomerase converts linoleic acid in salicornia clear oil into conjugated linoleic acid to obtain highly unsaturated active salicornia clear oil.

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Abstract

The invention discloses a biological extraction method of high-unsaturated-activity salicornia bigelovii seed oil, and belongs to the field of food biotechnology. The method comprises the following steps: firstly, shelling salicornia bigelovii seeds, drying, grinding, adding water for mixing, grinding until the particle size of pulp is 2-50 microns by a colloid grinder, compounding pectinase NCB-PE40 with cellulase AE80, and enzymatically hydrolyzing for breaking walls; secondly, adding 2.4 L of alcalase for enzymolysis, and centrifuging to obtain supernatant liquor to obtain salicornia bigelovii seed oil; finally, converting linoleic acid in the salicornia bigelovii seed oil into conjugated linoleic acid by linoleate isomerase to obtain the high-unsaturated-activity salicornia bigelovii seed oil. The method has the characteristics that the salicornia bigelovii seed oil is extracted by a bio-enzyme method, the process is simple, the conditions are mild, and the obtained salicornia bigelovii seed oil is high in unsaturated fatty acid content, high in activity, free of organic solvent residues, and environment-friendly.

Description

technical field [0001] The invention provides a biological extraction method of highly unsaturated active salicornia clear oil, which belongs to the field of food biotechnology. Background technique [0002] Commonly known as sea asparagus or sea beans, samphire is a delicious, juicy, salty and crispy green seasonal vegetable. It is also an oil crop that can be irrigated by seawater. The raw material plant Salicornia can be irrigated with salt water or sea water, and the yield per mu can reach 120-150kg, which is higher than that of sesame. Therefore, it is especially suitable for promotion in the vast saline-alkali areas of our country, especially for the development and utilization of saline-alkali wasteland and coastal tidal flats. Significance, in line with the national industrial policy and the world's low-carbon trend. [0003] The seeds of Salmonella are rich in oil, with an oil content as high as 26%-33%, including 12.5% ​​oleic acid, 74.0% linoleic acid, 2.6% linol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 李春阳李世科张红城
Owner NANJING FEIMA FOOD
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