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Application of bergamottin in preparation of medicines for preventing and treating diabetes

The technology of a diabetes drug and bergamot is applied in the application field of bergamot in the preparation of diabetes prevention and treatment drugs, which can solve problems such as unreported, and achieve the purpose of lowering blood sugar, increasing glucose tolerance and insulin sensitivity, and increasing glucose consumption. Effect

Inactive Publication Date: 2014-12-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For bergamot, its pharmacological activity is rarely reported, and for hypoglycemic activity, there is no report

Method used

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  • Application of bergamottin in preparation of medicines for preventing and treating diabetes
  • Application of bergamottin in preparation of medicines for preventing and treating diabetes
  • Application of bergamottin in preparation of medicines for preventing and treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] HepG2 cells were cultured in high-glucose DMEM containing 10% fetal bovine serum at 37 °C, 5% CO 2 Incubate in a cell incubator, replace with fresh culture medium every other day, and passage once every 2 to 3 days. During the experiment, HepG2 cells were seeded in 96-well plates, and blank control wells without cells were set. When the cells grew to 70-80% confluence, the original medium was discarded, washed twice with PBS buffer, replaced with serum-free 1640 medium containing 0.2% BSA, and drugs were added in groups. A DMSO solvent control group, a metformin control group (final concentration of 1 mmol / L) and different concentrations of bergamotin groups (final concentrations of 0.625 μg / mL, 1.25 μg / mL, 2.5 μg / mL and 5 μg / mL were set up) . After acting for 24 hours, use a microplate reader to detect the OD value of each well (detection wavelength: 505 nm), and use the glucose oxidase method to measure the glucose content of the culture solution in each well. Gluc...

Embodiment 2

[0021] KK mice were purchased from Beijing Huafukang Biotechnology Co., Ltd., and the special feed for feeding was also purchased from the company. All mice were raised in a constant temperature and humidity environment, provided with 12 hours of light and 12 hours of darkness, and fed a special diet 1K65 (crude fat%≧6.0, crude protein≧18.0). Eight C57 mice in the background group were purchased from Beijing Huafukang Biotechnology Co., Ltd., and the feed for feeding was also purchased from the company. Two groups of KK mice + water and KK mice + bergamot were set up in the KK mouse experiment, with 8 mice in each group, and BGM was bergamot at a dose of 50 mg / kg BW. All mice were free to ingest food and distilled water, and the treatment group was given medicine by gavage needle, and the KK mice+water group was given distilled water by gavage. The test lasted for 2 weeks, with 6 consecutive days of administration and 1 day of drug withdrawal. Fasting for 4 hours after admin...

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Abstract

The invention provides an application of bergamottin in preparation of medicines for preventing and treating diabetes and preparation of functional foods or health care products for preventing and treating diabetes. The bergamottin has the chemical formula of C21H22O4 and the relative molecular mass of 338.4. Studies prove that the bergamottin shows significant glucose consumption activity in human liver cancer HepG2 cells and has good concentration dependence, the action concentration is far lower than that of a positive medicine, namely metformin, and the efficacy is better than that of metformin. The bergamottin can significantly reduce blood glucose of diabetic KK-Ay mice in diabetic KK-Ay mouse bodies and increase glucose tolerance and insulin sensitivity. The studies about hypoglycemic activity of the bergamottin on intracellular level and in-vivo level show that the bergamottin shows significant hypoglycemic activity, can increase the glucose tolerance and insulin sensitivity and can be used as a treatment medicine or functional food or health care product for preventing and treating diseases related to abnormal glucose metabolism.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of natural source bergamot in the preparation of anti-diabetic drugs, in particular to the application of bergamot in reducing blood sugar at the cell level and the body level. Background technique [0002] Diabetes is a series of clinical syndromes caused by absolute or relative insufficiency of insulin in the body, which is closely related to genetics. The main clinical manifestations of diabetes are polydipsia, polyuria, polyphagia, and weight loss ("three more and one less"), as well as high blood sugar and glucose in the urine. The World Health Organization divides diabetes into four types: type 1 diabetes, type 2 diabetes, other types of diabetes and gestational diabetes. At present, type 1 and type 2 diabetes cannot be completely cured. The treatment of diabetes is mainly diet control combined with hypoglycemic drugs (for type 2 diabetes) or insulin supplementation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P3/10A23L1/29A23L33/00
Inventor 李鲜贾胜孙崇德陈昆松
Owner ZHEJIANG UNIV
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