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Preparation method of a novel corn protein source ethanol metabolism-promoting polypeptide

A technology for zein and ethanol metabolism, applied in the field of food biology, can solve the problems of insignificant functional components, high energy consumption, complex components, etc., and achieve the effects of high safety, reliable data and strong persuasion.

Active Publication Date: 2017-10-20
SOUTH CHINA UNIV OF TECH
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AI Technical Summary

Problems solved by technology

[0005] [1]. Publication No. CN 101411494A invention patent "Corn Sobering Peptide Beverage and Its Preparation Method" studied a preparation method of a compound formula corn peptide sobering drink, using corn yellow powder directly as the reaction substrate for biological enzymolysis, adding a variety of Composite agents such as sweeteners, sour agents, etc. are used to prepare functional hangover beverages, and no corresponding enzymatic research has been carried out on the two main proteins from different sources;
[0006] [2]. Publication No. CN 101455835A Patent of Invention "Protein Peptide Composite Nutrient Liquid Microspheres for Hangover and Preparation Method thereof" does not specify what kind of plant protein peptides, and also adds ingredients in multi-flavored Chinese medicine hangover prescriptions such as orange Skin water extract, etc., the composition is complex;
[0007] [3]. Application Publication No. CN 101837116A Invention Patent "A liver-protecting and anti-alcohol product with Saccharomyces cerevisiae as the main raw material" mainly studies the effect of multivitamins and reduced glutathione produced by yeast fermentation on liver protection. It also does not involve the relevant content of plant protein-derived polypeptides;
[0008] [4]. In the invention patent of application publication number CN 102028093A "Preparation method of corn hangover peptide", corn yellow powder is used as direct raw material, alkaline protease enzymatic hydrolysis combined with compound protease enzymatic hydrolysis, ultrafiltration, concentration, and spraying to obtain peptide powder, peptide The powder contains a large amount of corn yellow pigment, etc., the ingredients are complex, and the functional ingredients are not prominent;
[0009] [5]. In the invention patent of application publication number CN 102174625A "a preparation method of corn peptide", corn yellow powder is directly added to the prolamin system for enzymolysis, and high hydrolysis is obtained by virtue of the good solubility of prolamin in the system high-degree peptides, but the enzyme activity in the alcohol-soluble system is not good, and the energy consumption is high

Method used

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  • Preparation method of a novel corn protein source ethanol metabolism-promoting polypeptide
  • Preparation method of a novel corn protein source ethanol metabolism-promoting polypeptide

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Corn yellow powder raw material pretreatment to remove starch: corn yellow powder (mass 100g) 1:6 (v / v) add water, add α-amylase (10 mg / g) at pH 7.5, temperature 50 ° C, water bath for 40 min to remove residue starch.

[0035] (2) Extraction of gliadin and glutenin: wash the maize flour from residual starch three times with water, add ethanol solution (77%, v / v) at 1:12 (m / v, unit: g / ml), After stirring for 3.0 hours, the supernatant and residue obtained by centrifugation were processed separately: a. The supernatant was diluted with ethanol to a concentration of 45% (v / v) and then centrifuged at 8,000 rpm (4°C, 15 min) to obtain prolamin; b. The residue was washed with water After 3 times, add NaOH solution (0.5%, m / m) according to the ratio of material to liquid 1:7 (v / v), stir at 38°C for 1.5h, and centrifuge the supernatant to adjust the pH to 4.0 to precipitate precipitation, 8,000rpm ( 4°C, 15 min) to obtain gluten by centrifugation.

[0036] (3) Preparation...

Embodiment 2

[0039] (1) Corn yellow powder raw material pretreatment to remove starch: corn yellow powder (mass 100g) 1:9 (v / v) add water, add α-amylase (40mg / g) at pH 7.5, temperature 55 ℃, water bath 60min centrifuge to remove residue starch.

[0040] (2) Extraction of gliadin and glutenin: wash the maize flour from residual starch three times with water, add ethanol solution (80%, v / v) at 1:16 (m / v, unit: g / ml), After stirring for 3.0 hours, the supernatant and residue obtained by centrifugation were processed separately: a. The supernatant was diluted with ethanol to a concentration of 45% (v / v) and then centrifuged at 8,000 rpm (4°C, 15 min) to obtain prolamin; b. The residue was washed with water After 3 times, add NaOH solution (0.8%, m / m) according to the ratio of material to liquid 1:9 (v / v), stir at 48°C for 3.0h, and centrifuge the supernatant to adjust the pH to 4.8 to precipitate a precipitate, 8,000rpm ( 4°C, 15 min) to obtain gluten by centrifugation.

[0041] (3) Preparat...

Embodiment 3

[0044] (1) Corn yellow powder raw material pretreatment to remove starch: add corn yellow powder (mass 100g) 1:8 (v / v) with water, add α-amylase (25mg / g) at pH 7.5, temperature 52°C, centrifuge in water bath for 50min to remove residue starch.

[0045] (2) Extraction of gliadin and glutenin: Wash the maize flour from residual starch three times with water, add ethanol solution (78%, v / v) at 1:13 (m / v, unit: g / ml), After stirring for 3.0 hours, the supernatant and residue obtained by centrifugation were processed separately: a. The supernatant was diluted with ethanol to a concentration of 45% (v / v) and then centrifuged at 8,000 rpm (4°C, 15 min) to obtain prolamin; b. The residue was washed with water After 3 times, add NaOH solution (0.7%, m / m) according to the ratio of material to liquid 1:8 (v / v), stir at 45°C for 2.0h, and centrifuge the supernatant to adjust the pH to 4.5 to precipitate precipitation, 8,000r( 4°C, 15 min) to obtain gluten by centrifugation.

[0046] (3)...

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Abstract

The invention discloses a novel method for preparing ethanol metabolism-promoting polypeptide derived from corn protein. The method comprises: pretreatment of corn yellow powder raw material to remove starch; extraction of prolamin and glutenin; mixed enzymatic hydrolysis product of prolamin and glutenin preparation; the supernatant enzymatic hydrolyzate was freeze-dried to obtain the ethanol metabolism-promoting polypeptide derived from corn protein. The obtained product is used as the main functional component of the ethanol metabolism-promoting health-care product to reduce the damage of ethanol to the human body. The active peptide has been verified by the alcohol dehydrogenase (ADH) kit and the police alcohol blowing tester, and it is confirmed that the activity of alcohol dehydrogenase can be increased by 32%~45%. Compared with the peptide obtained from a single gliadin enzymatic hydrolysis product, increasing the proportion of gluten in the enzymatic hydrolysis raw material within a certain range can make the obtained crude polypeptide have a greater activation effect on alcohol dehydrogenase ADH and a faster metabolism of ethanol. The molecular weight of the target active peptide was generally lower than 1kDa as determined by gel chromatography analysis.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a preparation method of a novel corn protein source ethanol metabolism-promoting polypeptide. Background technique [0002] Wine is widely consumed by people all over the world as a kind of cosmopolitan beverage, and my country has a long history of preparing and drinking alcoholic beverages. Chinese medicine advocates that moderate drinking has the benefits of dredging tendons and collaterals, promoting blood circulation and removing blood stasis, while heavy drinking "will cause bloating, gas upside down, fullness in the chest, liver and gallbladder." Especially for modern people, under the change of living environment and lifestyle, drinking opportunities have greatly increased, and the losses and injuries caused by drinking are also countless. Drinking a lot of alcohol can lead to memory loss, nausea and vomiting, and even seriously endanger life safety and social property ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 任娇艳刘鹏赖婷林泽华张榕赵谋明
Owner SOUTH CHINA UNIV OF TECH
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