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Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method

A technology for photosynthetic microorganisms and surface growth, applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc. Water and other problems, to achieve the effect of good water absorption/permeability

Inactive Publication Date: 2015-02-04
STATE DEV & INVESTMENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of PBR is usually expensive, high operating cost, difficult to maintain, difficult to scale up, and the yield is 5-30g / m 2 / d, far lower than the theoretical prediction value of 100-200g / m 2 / d, the theoretical calculation target of industrialization cannot be reached, and the light energy utilization rate is low, which is the most important direct constraint factor for the large-scale cultivation of microalgae to realize industrialization, which still needs to be improved
[0004] In recent years, a kind of semi-dry culture system has appeared, and there have been patent reports, all of which use one or more of filter paper, filter cloth, sponge, foam plastic, fiber fabric (such as canvas) as the matrix material, but this type Soft materials have the following disadvantages: 1) low mechanical strength, requiring complex supports, not suitable for large size and space height enlargement, and can only be made into low and thin structures; 2) uneven surface humidity, poor growth of microalgae firm; and 3) it is not resistant to recycling and needs to be replaced frequently, which increases the cost, 4) it is easy to drop impurities such as fibers; in addition, 5) when these flexible materials are used as the surface material of the culture plate, their biocompatibility and chemical corrosion resistance Sexuality and other aspects have certain limitations
6) When these materials are used as the surface-grown photobioreactor, due to the height difference in the vertical direction, the hydrostatic pressure near the bottom is relatively high, the water seepage is fast and water accumulation occurs, so that the microalgae and algae are easily destroyed. Rinse off (fails to attach and grow evenly)
Therefore, it is currently impossible for this type of culture system to enter large-scale production.

Method used

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  • Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method
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  • Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] combine figure 2 with image 3 Be explained, image 3 Showing the surface growth type culture system 1000a of the present invention, the surface growth type culture system 1000a may comprise a plurality of such as figure 2 Shown are the culture units 101 , the light source device 8 disposed between the culture units 101 , and the culture solution supply device 9 for supplying the culture solution to the culture units 101 .

[0088] Such as figure 2 with 3 As shown, the fixing device 2 includes opposite fixing components 21 and 22, and a hole 23 for fixing the culture plate 1 is formed between the fixing components 21 and 22, and the culture plate 1 can also be taken out from the hole 23 upwards.

[0089] image 3 A plurality of culture units 101 in the set are arranged parallel to each other and separated by a distance. At least one light source device 8 is arranged between every two culture units 101 . The light source device can be a double-sided light source ...

Embodiment 2

[0094] combine figure 2 with Figure 4 Another cultivation system 1000b of the present invention is illustrated. Figure 4 A surface-grown culture system 1000b of the present invention is shown. and image 3 Similar to the illustrated embodiment, the culture system 1000b may also include one or more figure 2 The culture unit 101. Specifically, the culture system 1000b may include a core-shell structure culture plate 1, a fixing device 2, a culture solution recovery device 3, a liquid supply device 4, a culture solution pool 6, a pressure tank 11, a compressed air source 12, a liquid level gauge 13, Stop valve 14, stop valve 15, pressure reducing valve 16, exhaust valve 17, and pressure gauge 18.

[0095] Figure 4 The surface growth culture system 1000b includes a culture solution circulation device 9b, and the air compression method can be optionally used in the culture solution circulation device 9b. The device used in this air compression method is other optional p...

preparation example

[0115] Obtain the core-shell structure culture plate ① of the present invention according to the aforementioned preparation method 1;

[0116] According to the preparation method 2, the core-shell structure culture plate of the present invention was prepared with molecular sieve ZSM-5 ②.

[0117] The dimensions of the core-shell structure culture plates ① and ② are both 1 meter*2 meters*3 centimeters.

[0118] Set up 3 groups of controls:

[0119] Group 1: control canvas (32 pieces), the size is equivalent to the core-shell structure culture plate 1, and the sandwich layer has a sponge water-absorbing material.

[0120] Group 2: According to the aforementioned preparation method 1, a porous plate was made with molecular sieves and alumina stone, but no channel 111 was formed, and the control plate ① was obtained;

[0121] Group 3: A block made of molecular sieve ZSM-5 was clamped between two water-permeable ceramic plates to obtain a control plate ②.

[0122] The size of th...

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PUM

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Abstract

The invention discloses a surface growth type photosynthetic microorganism culture plate having a core-shell structure, which comprises a shell plate arranged outside and a core part arranged in the shell plate, wherein the shell plate is of a porous plate-shaped structure made from a rigid water seepage material; the filling core is made from a water retention and water seepage material; and the filling core is arranged in the shell plate. According to the core-shell type composite culture plate disclosed by the invention, photosynthetic microorganisms are attached to the surface, a nutrient solution for supporting growth of the photosynthetic microorganisms is retained in the filling core, and the nutrient solution gradually permeates to the surface of the shell plate from the filling core and then is supplied for sustainable reproduction of the photosynthetic microorganisms. In addition, the invention further discloses a culture unit comprising the core-shell type composite culture plate, a culture system, a culture method and application thereof.

Description

technical field [0001] The invention relates to a surface growth type photosynthetic microorganism culture plate with a core-shell structure, a culture unit comprising the culture plate, a surface growth type culture system, a method and an application thereof. Background technique [0002] Photosynthetic microorganisms are a kind of microorganisms that use light as the only or main source of energy to grow and reproduce, including microalgae, cyanobacteria and other microorganisms that contain chlorophyll and can perform photosynthesis. Microorganisms have important biological utilization value. According to current literature reports, algae are rich in protein and can be used as aquatic bait or feed for livestock and poultry (such as spirulina); more importantly, some microalgae can synthesize a large amount of secondary metabolites under specific conditions, such as oil, carotenoid, Polysaccharides, etc. These substances are often biologically active substances with extre...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04C12N1/12C12N1/20
CPCC12M41/40C12M31/10C12M41/12
Inventor 胡强喻正保刘倩
Owner STATE DEV & INVESTMENT
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