Extracting method of placenta

An extraction method and technology of placenta, which is applied in the field of extracting placenta, can solve the problems of many technical parameter control factors, affecting the physical and chemical indicators of finished products, and being unsuitable for industrial production, so as to avoid potential safety hazards, have better taste, and save concentration time Effect

Inactive Publication Date: 2015-02-25
DELISI GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most placental extract products are processed by vacuum freeze-drying to maintain their activity, but freeze-drying often causes the porous layer to collapse, affecting the physical and chemical indicators of the finished product. Therefore, excipients should be added to reduce the low-temperature damage to the finished product. There are many control factors and process links in the process of converting liquid materials into solid powder freeze-drying technology, and freeze-drying consumes a lot of energy, high production costs, and long cycles. It is usually suitable for laboratory preparation and is really used for large-scale preparation. The production process is less, not suitable for industrial production

Method used

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  • Extracting method of placenta
  • Extracting method of placenta
  • Extracting method of placenta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Select fresh pig placenta or pig embryos qualified through inspection and quarantine, remove blood, fat and umbilical cord, wash, mince with a meat grinder as raw material, then add purified water 7 times the weight of the raw material and stir evenly, Under the condition of 95° C., cook for 35 minutes to obtain the pretreated raw material.

[0029] (2) the pretreated raw material is cooled to 52 DEG C, and the pH value is adjusted to 7.9 with food-grade sodium hydroxide, and 1.2% of the weight of the raw material is added, and the enzyme activity is 18U / mg alkaline protease, and then The temperature was kept at 52°C for 5 hours, and the pH value of the system was kept stable during the reaction. After the enzymolysis, the temperature of the obtained enzymolysis solution was raised to 92°C and kept for 15 minutes to inactivate the enzyme.

[0030] (3) Centrifuge the enzymolyzed solution after deactivating the enzyme to remove fat and solids to obtain placental fluid...

Embodiment 2

[0040] (1) Select fresh pig placenta or pig embryos qualified through inspection and quarantine, remove blood, fat and umbilical cord, wash, mince with a meat grinder as raw material, then add purified water with 8 times the weight of the raw material and stir evenly. Under the condition of 98° C., cook for 30 minutes to obtain the pretreated raw material.

[0041] (2) the pretreated raw material is cooled to 55° C., the pH value is adjusted to 8.2 with food-grade sodium hydroxide, and 2% of the raw material weight is added, and the enzyme activity is 20 U / mg alkaline protease, and then The temperature was kept at 55°C for 4 hours, and the pH value of the system was kept stable during the reaction. After the enzymolysis was completed, the temperature of the obtained enzymolysis solution was raised to 95°C and kept for 10 minutes to inactivate the enzyme.

[0042] (3) Centrifuge the enzymolyzed solution after deactivating the enzyme to remove fat and solids to obtain placental ...

Embodiment 3

[0051] (1) Select fresh pig placenta or pig embryos qualified through inspection and quarantine, remove blood, fat and umbilical cord, wash, mince with a meat grinder as raw material, then add purified water 7.5 times the weight of the raw material and stir evenly, Under the condition of 96° C., cook for 32 minutes to obtain the pretreated raw material.

[0052] (2) the pretreated raw material is cooled to 53°C, the pH value is adjusted to 8.0 with food-grade sodium hydroxide, and 1.5% of the raw material weight is added, and the enzyme activity is 22U / mg alkaline protease, and then The temperature was kept at 53°C for 4.5 hours, and the pH value of the system was kept stable during the reaction. After the enzymolysis, the temperature of the obtained enzymolysis solution was raised to 93°C and kept for 13 minutes to inactivate the enzyme.

[0053] (3) Centrifuge the enzymolyzed solution after deactivating the enzyme to remove fat and solids to obtain placental fluid.

[0054]...

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Abstract

The invention discloses an extracting method of placenta. The extracting method comprises the following steps: (1) selecting and mincing a fresh pig uterus or pig embryo as a raw material, adding purified water to uniformly stir and cooking for 30-35 minutes; (2) cooling and regulating the pH value to 7.8-8.3, adding alkaline protease with active content of 15-20U / mg, reacting for 4-5 hours at a temperature of 50-55 DEG C, and keeping for 10-15 minutes at 90-95 DEG C; (3) centrifuging to remove fat and solid matters to obtain a placenta solution; (4) adding trichloroacetic acid, and then centrifuging and removing hybrid protein to obtain a clear solution; (5) adding active carbon to deodorize, and removing the active carbon to obtain filtrate; (6) using a nanofiltration membrane to separate, desalting and concentrating to obtain a concentrated solution; and (7) spraying and drying to obtain the placenta. The extracting method of the placenta disclosed by the invention is short in production period, low in cost, free of pollution, high in product purity, small in molecular weight, good in human body absorption, good in mouthfeel, and capable of being widely applied to the fields such as food, healthcare products, and medicines.

Description

technical field [0001] The invention relates to the field of deep processing of pig placenta, in particular to a method for extracting placenta by combining biotechnology and chemical technology. Background technique [0002] Pig placenta is a by-product of the pig breeding process, which contains a lot of protein, heme, human growth factor and other trace elements, and its nutritional value is very considerable. Pig placenta has the functions of nourishing blood and replenishing essence, promoting bone marrow growth, promoting ovulation, and improving reproductive ability. It is a regulator of the body's immune function and a nutritional and beauty care product. [0003] In recent years, two new types of placental biological products, animal placenta hydrolyzate and placenta extract, have emerged at home and abroad, but the above two animal placenta preparations have their own disadvantages. The former destroys the natural active substances in the placenta during the hydrol...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 张晓东李敏郑乾坤
Owner DELISI GROUP
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