Method for intensifying starting performance of anaerobic ammonia oxidation process by using ultrasonic wave

A technology of anammox and anammox bacteria, which is applied in the field of new wastewater biological denitrification technology, can solve the problems of long start-up period of anammox process, low growth rate of anammox bacteria, and high environmental sensitivity , to achieve the effect of enhancing the activity and the competitive advantage of the flora, shortening the start-up cycle and high biological activity

Inactive Publication Date: 2015-03-04
HEBEI UNIV OF TECH
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Problems solved by technology

[0005] In order to solve the problems of low growth rate of anammox bacteria, high environmental sensitivity and too long start-up period of anammox pro...

Method used

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  • Method for intensifying starting performance of anaerobic ammonia oxidation process by using ultrasonic wave

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Embodiment 1

[0023] The aerobic activated sludge in the CASS tank of a municipal sewage treatment plant was used as the inoculum. The ratio of reactor height to inner diameter is 1.5, and the effective volume is 2.4L. Firstly, the fixed-bed reactor is filled with honeycomb polypropylene (PP) packing, the selected PP honeycomb packing has a diameter of 2.5 cm and a filling ratio of 85%. Then the collected aerobic activated sludge was rinsed 3 times with water. After rinsing, the sludge mixed liquor suspended solids concentration (MLSS) was 1.86g / L, and the mixed liquor volatile suspended solids concentration (MLVSS) was 1.53g / L. Take 2L of sludge mixture and inoculate it into the reactor. The influent is simulated wastewater, prepared according to the main components of the anammox bacteria culture medium, mainly containing ammonia nitrogen and nitrite nitrogen, and does not contain organic matter. With 95%Ar / 5%CO 2 The mixed gas is used to aerate the simulated wastewater for 10 minutes...

Embodiment 2

[0025]Nitrifying sludge from an aquaculture sewage treatment plant using A / O process was used as the inoculum. After 2 times of rinsing, the MLSS and MLVSS of the inoculum sludge were 2.16g / L and 1.63g / L, respectively. The selected PP honeycomb filler has a diameter of 1.5cm. The reaction temperature is 37°C, and the pH value is 7-7.8. During the operation of the device, the cultured sludge is subjected to ultrasonic irradiation twice a day, the frequency of each ultrasonic irradiation is 20kHz, and the intensity is 0.3w / cm 2 , the irradiation time is 5min. The remaining steps are the same as in Example 1. After 51 days of continuous operation, the anammox sludge is domesticated and mature, and the anammox process is successfully started.

Embodiment 3

[0027] Granular sludge from an upflow anaerobic sludge bed (UASB) was used as the inoculum. After one rinse, the MLSS and MLVSS of the inoculum sludge were 2.85g / L and 2.13g / L, respectively. The diameter of the selected PP honeycomb filler is 1cm. The pH value of the reactor mixture is 7.5-8. During the start-up process of the process, the cultured sludge is irradiated with ultrasound once a day, and the frequency used for each ultrasound irradiation is 50kHz, and the intensity is 0.7w / cm 2 , the irradiation time is 3min. The rest of the steps were the same as in Example 1. After 62 days of operation, the anammox process was started successfully, and the anammox sludge was domesticated and mature, and the anammox bacteria were the dominant bacteria in the sludge.

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Abstract

The invention belongs to novel wastewater biological nitrogen removal processes, and discloses a method for intensifying the starting performance of an anaerobic ammonia oxidation process which takes ordinary activated sludge as an inoculum by using ultrasonic waves. An ultrasonic generator main machine is arranged outside an anaerobic ammonia oxidation starting reactor, and an ultrasonic wave vibrator is submerged below the liquid level inside the reactor. By irradiating culture sludge of the anaerobic ammonia oxidation process starting process by using low-intensity ultrasonic waves, the cytomembrane permeability, the electron transfer speed and the functional enzymatic activity of anaerobic ammonium oxidation bacteria are improved, so that the activity and flora competitive advantages of the anaerobic ammonium oxidation bacteria in the culture sludge are improved, and rapid starting of the process is achieved. By adopting the method, both the starting period of the anaerobic ammonia oxidation process is shortened, and the anaerobic ammonia oxidation bacteria can be domesticated in the activated sludge, and a technical support and stain reservation are provided for the industrialization process of the anaerobic ammonia oxidation process. The method has the advantages that the reaction condition is gentle and free of secondary pollution, the device is simple in structure and easy to operate, and the bacteria grow fast and are high in bioactivity, resistant to load impact and good in stability.

Description

technical field [0001] The invention belongs to a novel waste water biological denitrification process. Specifically, it relates to a method for rapidly starting an anaerobic ammonium oxidation process by using ultrasonic irradiation under the precondition that ordinary activated sludge is used as an inoculum. Background technique [0002] In recent years, water eutrophication caused by nitrogen and phosphorus pollution has become increasingly serious, and ecological disasters such as algal blooms and red tides have occurred frequently. Ammonia nitrogen is the primary pollutant of water nitrogen pollution, so the treatment of ammonia nitrogen-containing wastewater is an important link in the prevention and control of water eutrophication. When the traditional wastewater biological denitrification process treats high-concentration ammonia nitrogen, a large amount of organic carbon needs to be invested as an electron donor in the denitrification stage, and aeration and oxygen...

Claims

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Application Information

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IPC IPC(8): C02F3/28
Inventor 汪涛张沙王志强张长平
Owner HEBEI UNIV OF TECH
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