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Preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin

A technology of cyhalothrin and molecular imprinting, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of difficult elution, low yield, slow mass transfer rate of molecularly imprinted polymers, etc., and achieve good monodispersity performance, Effect of high sensitivity and wide detection range

Inactive Publication Date: 2015-03-11
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to solve the disadvantages of slow mass transfer rate, difficult elution, and low yield of molecularly imprinted polymers prepared by conventional polymerization methods, surface molecular imprinting technology (SMIT) emerged as the times require.

Method used

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  • Preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin
  • Preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin
  • Preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin

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Experimental program
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Effect test

Embodiment 1

[0033] SiO prepared by st?ver method 2 Nanospheres were added to every 100 mL of ethanol and distilled water ratio of 10:3, (v / v) 5.0 mL of ammonia-water mixture, and stirred continuously after 30 minutes of sonication; - Aminopropyltriethoxysilane (APTS) was mixed and continuously stirred for 24 hours, and then slowly dropped into the above dispersion liquid. After the dropwise addition, it was kept under room temperature and kept stirring for another 24 hours; after the reaction, ethanol After being washed with distilled water for several times, it was collected by a high-speed centrifuge and dried overnight in a vacuum oven at 40°C to obtain SiO 2 FITC-APTS microspheres. FTIR as figure 2 shown.

Embodiment 2

[0035] The SiO obtained in 200 mg embodiment 1 2 FITC-APTS microspheres were ultrasonically dispersed in 70 mL of acetonitrile, and 0.50 mmol of template molecules (cyhalothrin, LC), 2.0 mmol of functional monomers (acrylamide, AM) and 0.4 mL of cross-linking agent (divinylbenzene , DVB). Self-assembled at room temperature for 12 hours, after the functional monomers and template molecules fully interacted, 2.5% of the initiator azobisisobutyronitrile (AIBN) based on the total amount of the monomers was added. Air was purged by bubbling nitrogen gas for 15 minutes, and sealed under nitrogen atmosphere. Thermally initiated polymerization was used, and it was placed in a constant temperature oil bath at 60°C for 24 h to react. After the polymerization was completed, a pale yellow precipitated polymer was obtained. Soxhlet extraction and elution with a solution of methanol: acetic acid at 9:1 (V / V) to remove cyhalothrin, and then soak the precipitate repeatedly with methanol sol...

Embodiment 3

[0037] The SiO obtained in 200 mg embodiment 1 2 FITC-APTS microspheres were ultrasonically dispersed in 70 mL of acetonitrile, and 0.75 mmol of template molecule (cyhalothrin, LC), 3.0 mmol of functional monomer (acrylamide, AM), and 0.6 mL of cross-linking agent (divinyl Benzene, DVB). Self-assembled at room temperature for 12 hours, after the functional monomers and template molecules fully interacted, 2.5% of the initiator azobisisobutyronitrile (AIBN) based on the total amount of the monomers was added. Air was purged by bubbling nitrogen gas for 15 minutes, and sealed under nitrogen atmosphere. Thermally initiated polymerization was used, and it was heated in a constant temperature oil bath at 60 °C for 24 h. After the polymerization was completed, a pale yellow precipitated polymer was obtained. Soxhlet extraction and elution with a solution of methanol: acetic acid at 9:1 (V / V) to remove cyhalothrin, and then soak the precipitate repeatedly with methanol solution to ...

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Abstract

The invention relates to a preparation method of a molecularly imprinted material for fluorescent selective recognition of cyhalothrin and belongs to the technical field of environment function material preparation. By the utilization of a precipitation polymerization method, a fluorescent molecularly imprinted polymer is synthesized by using Lambda-cyhalothrin as a template molecule, using SiO2 sub-microspheres as a carrier, using acrylamide (AM) as a functional monomer, using fluorescein isothiocyanate (FITC) as a fluorescent reagent, using divinyl benzene (DVB) as a cross-linking agent and using 2,2'-azodiisobutyronitrile (AIBN) as an initiator. The prepared fluorescent molecularly imprinted polymer has high sensitivity and has high performance of selective recognition of cyhalothrin. Through detection results of a liquor sample, it proves that the material prepared by the method can be used in quantitative detection of practical complex samples.

Description

technical field [0001] The invention relates to a preparation method suitable for fluorescence selective recognition and detection of cyhalothrin core-shell imprinted polymer submicron spheres, belonging to the technical field of environmental functional material preparation. Background technique [0002] Molecular imprinting technology (MIT) refers to the technology of preparing polymers with a certain selective recognition ability. The polymer is specific to a specific target molecule (called template molecule or imprinting molecule) in terms of spatial structure and binding site. With memory function, the polymers synthesized by this technology are called molecular imprinting polymers (molecular imprinting polymers, referred to as MIPs). MIPs prepared by molecular imprinting technology have high selectivity and good affinity for target molecules. Due to the cross-linking agent added in the preparation process, the prepared MIPs have a certain rigid structure, so the prop...

Claims

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Application Information

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IPC IPC(8): C08F220/56C08F212/36C08F2/44C08K9/06C08K9/04C08K3/36C08J9/26G01N21/64
Inventor 王吉祥闫永胜高林沈洪强李根刘树成李春香
Owner JIANGSU UNIV
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