The method of using RNAi to effectively control agricultural pest mites

A double-way tube and sealing film technology, applied in the field of agricultural biology, can solve the problems of difficult operation of RNAi molecular technology and less research on mites, and achieve the effects of reducing experimental costs, reducing mechanical damage, and reducing dosage
CN104404048BInactive Publication Date: 2019-02-05SHIHEZI UNIVERSITY
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHIHEZI UNIVERSITY
Publication Date
2019-02-05
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the field of a biotechnology, specifically to a method for controlling leaf mites by silencing an energy metabolism gene. The method provided by the invention comprises the following steps: leaf mite lethal gene AK is selected as an RNAi target gene; and the RNAi target gene is connected to a plasmid vector L4440 and is transformed to escherichia coli HT115 so as to obtain a bacterial strain for expression of AK gene dsRNA. The method is an important supplement for a leaf mite control method by mainly using a pesticide. By the RNAi technology, expression of the energy metabolism gene in leaf mites is interfered. The method provides a technical support for controlling leaf mites.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of agricultural biotechnology; more specifically, the present invention relates to a target gene and dsRNA thereof based on RNAi technology for preventing and controlling agricultural pest mite, and describes a method for synthesizing the target gene into a double-stranded RNA preparation and directly applying it to the control of agricultural pest mite . technical background

[0002] In my country, there is currently no effective control method for agricultural pest mites, and spraying insecticides is still the main control method. The indiscriminate application of highly toxic and high-residue pesticides has not only led to the decline in the quality of crop products, the increase in the resistance of pest mites, but also endangered people's lives and health. Therefore, there is an urgent need to develop safe, effective and new strategies to control the harm of pest mite populations.

[0003] The phenomenon of RNA...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More