A method for making a guided tissue regeneration membrane with an absorbable antibacterial bone-like structure

A technology for guiding tissue regeneration and making methods, applied in the field of biomedical materials, can solve the problems of poor mechanical properties, unable to maintain bone defect space, insufficient osteoinductive activity, no antibacterial properties, etc. Easy to absorb and shape

Active Publication Date: 2016-08-17
李柏霖 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problems that the currently clinically applied bone tissue-guided regeneration collagen membrane has poor mechanical properties, cannot maintain the bone defect space, has no antibacterial properties, and has insufficient osteoinductive activity, the present invention provides a bone-like structure mineralized collagen loaded with antibacterial drugs Membrane production method:

Method used

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  • A method for making a guided tissue regeneration membrane with an absorbable antibacterial bone-like structure
  • A method for making a guided tissue regeneration membrane with an absorbable antibacterial bone-like structure
  • A method for making a guided tissue regeneration membrane with an absorbable antibacterial bone-like structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation of Decellularized Bovine Pericardium Collagen Fibrous Tissue Regeneration Guiding Membrane with Mineralized Osteoid Structure

[0057] A: Obtain fresh bovine pericardium, select a uniform anterior wall, remove fat tissue, wash with PBS buffer three times, and store in liquid nitrogen for later use;

[0058] B: Soak bovine pericardium membrane trimmed to a certain size with PBS buffer, then place it in a -20°C refrigerator for 4 hours, and finally place it in a 37°C water bath for 30 minutes, then sonicate for 2 minutes, and freeze and thaw three times;

[0059] C: Rinse the pericardium treated in step B with 100ml PBS buffer solution for 24 hours, change the solution every 6 hours during this period, and shake it in a low-temperature shaker at 4°C;

[0060] D: Immerse the pericardium treated in step C in 0.25% TritonX-100 PBS buffer, shake at 37°C for 24 hours, and sonicate for 2 minutes;

[0061] E: The pericardium treated in step D was rinsed w...

Embodiment 2

[0068] Example 2: Preparation of decellularized bovine pericardium collagen fibrous tissue regeneration guiding membrane with antibacterial mineralized bone-like structure

[0069] A: Obtain fresh bovine pericardium, select a uniform anterior wall, remove fat tissue, wash with PBS buffer three times, and store in liquid nitrogen for later use;

[0070] B: Soak bovine pericardium membrane trimmed to a certain size with PBS buffer, then place it in a -20°C refrigerator for 4 hours, and finally place it in a 37°C water bath for 30 minutes, then sonicate for 2 minutes, and freeze and thaw three times;

[0071] C: Rinse the pericardium treated in step B with 100ml PBS buffer solution for 24 hours, change the solution every 6 hours during this period, and shake it in a low-temperature shaker at 4°C;

[0072] D: Immerse the pericardium treated in step C in 0.25% TritonX-100 PBS buffer, shake at 37°C for 24 hours, and sonicate for 2 minutes;

[0073] E: The pericardium treated in ste...

Embodiment 3

[0081] Example 3: Preparation of decellularized and demineralized bovine lamellar collagen fibrous tissue regeneration guiding membrane with mineralized bone-like structure

[0082] A: Obtain the tubular bones of fresh bovine limbs, remove the periosteum and other soft tissues, saw them into a certain size and shape under cooling conditions, remove the obvious bone marrow tissue, and store them in liquid nitrogen for later use;

[0083] B: Put a bone of a certain size into 200mL of a mixed solution containing 0.5M EDTA and 0.25% TritonX-100, the pH of the EDTA solution is 8.0, decalcify for 1 week under constant stirring at room temperature, until the bone becomes soft , the pin can be easily pierced. The solution was changed once a day;

[0084] C: The demineralized bone slices treated in B were frozen in a -20°C refrigerator, and then cut into 200 μm thick membranes with a frozen tissue slicer;

[0085] D: Immerse the demineralized bone treated in step C in 0.25% TritonX-1...

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Abstract

The invention provides a method for preparing an absorbable and antibacterial guided tissue regeneration membrane for a bone-like structure, and belongs to the field of biological medical materials. The method comprises the following steps: firstly, preparing a cell free bovine pericardial collagen fiber membrane by adopting a repeated freeze-thaw method and a surface active agent TritonX-100, or preparing a demineralized cell free collagen fiber membrane of a cattle lamellar bone by using a method of EDTA decalcification by using the surfactant TritonX-100; treating by using sodium trimetaphosphate to obtain a phosphorylated collagen fiber membrane; with the assistance of a direct-current electric field, mineralizing in agar hydrogel containing calcium and phosphate, thereby obtaining a mineralized collagen membrane with a bone-like structure with certain hardness; and finally soaking the mineralized collagen membrane into a minocyline solution, and preparing an antibacterial bone-like structured composite membrane structure by virtue of the characteristics of combined minocyline and hydroxyapatite. The guided tissue regeneration membrane provided by the invention has a bone-like structure, is capable of maintaining a bone defect space, is good in operation forming property and antibacterial property, and can be applied to clinical practice for bone defect remediation and reconstruction in periodontal departments, dental implant mediation and occlusalf surfaces.

Description

technical field [0001] The invention belongs to biomedical material technology, and relates to a preparation method of an absorbable film material. It is used to guide the regeneration of bone tissue and periodontal tissue in clinical oral cavity, and to regenerate bone tissue in repair and reconstruction surgery. Background technique [0002] Membrane-guided bone regeneration (GBR): refers to a new technology that establishes a physical barrier in the bone defect area to isolate the bone defect area from the surrounding soft tissue that interferes with bone growth, so as to promote new bone growth. In 1957, "American Journal of Surgery" reported for the first time that a hard plastic was covered on the experimental defect of the dog's tibia. After 10 weeks, new bone was found to grow into the hard plastic-covered bone wound, and the amount of bone growth was determined by the membrane. The size of the space under the object. Therefore, it is considered that preventing sof...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/02A61L31/16
Inventor 李柏霖李全利
Owner 李柏霖
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