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Method for preparing absorbable and antibacterial guided tissue regeneration membrane for bone-like structure

A technology that guides tissue regeneration and manufacturing methods, applied in the field of biomedical materials, can solve the problems of no antibacterial properties, insufficient osteoinductive activity, poor mechanical properties and unable to maintain bone defect space, etc., to achieve convenient molding and operation, and facilitate bone repair space , the effect of inhibiting bone resorption

Active Publication Date: 2015-03-25
李柏霖 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problems that the currently clinically applied bone tissue-guided regeneration collagen membrane has poor mechanical properties, cannot maintain the bone defect space, has no antibacterial properties, and lacks osteoinductive activity, the present invention provides a bone-like structure loaded with antimicrobial drug mineralization Collagen film production method;

Method used

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  • Method for preparing absorbable and antibacterial guided tissue regeneration membrane for bone-like structure
  • Method for preparing absorbable and antibacterial guided tissue regeneration membrane for bone-like structure
  • Method for preparing absorbable and antibacterial guided tissue regeneration membrane for bone-like structure

Examples

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Effect test

Embodiment 1

[0030] Example 1: Preparation of Decellularized Bovine Pericardium Collagen Fibrous Tissue Regeneration Guiding Membrane with Mineralized Osteoid Structure

[0031] A: Obtain fresh bovine pericardium, select a uniform anterior wall, remove fat tissue, wash with PBS buffer three times, and store in liquid nitrogen for later use;

[0032] B: Soak bovine pericardium trimmed to a certain size with PBS buffer, then place it in a -20°C refrigerator for 4 hours, and finally place it in a 37°C water bath for 30 minutes, then sonicate for 2 minutes, and freeze and thaw repeatedly 3 times;

[0033] C: Rinse the pericardium treated in step B with 100ml PBS solution for 24 hours, change the solution every 6 hours during this period, and shake it in a low-temperature shaker at 4°C;

[0034] D: Immerse the pericardium treated in step C in 0.25% TritonX-100 PBS solution, shake at 37°C for 24 hours, and sonicate for 2 minutes during the process;

[0035] E: The pericardium treated in step ...

Embodiment 2

[0042] Example 2: Preparation of decellularized bovine bovine pericardium collagen fibrous tissue regeneration guiding membrane with antibacterial mineralized bone-like structure

[0043] A: Obtain fresh bovine pericardium, select a uniform anterior wall, remove fat tissue, wash with PBS buffer three times, and store in liquid nitrogen for later use;

[0044] B: Soak bovine pericardium trimmed to a certain size with PBS buffer, then place it in a -20°C refrigerator for 4 hours, and finally place it in a 37°C water bath for 30 minutes, then sonicate for 2 minutes, and freeze and thaw repeatedly 3 times;

[0045] C: Rinse the pericardium treated in step B with 100ml PBS solution for 24 hours, change the solution every 6 hours during this period, and shake it in a low-temperature shaker at 4°C;

[0046] D: Immerse the pericardium treated in step C in 0.25% TritonX-100 PBS solution, shake at 37°C for 24 hours, and sonicate for 2 minutes during the process;

[0047] E: Rinse the...

Embodiment 3

[0055] Example 3: Preparation of decellularized and demineralized bovine lamellar collagen fibrous tissue regeneration guiding membrane with mineralized bone-like structure

[0056] A: Obtain the tubular bones of fresh bovine limbs, remove the periosteum and other soft tissues, saw them into a certain size and shape under cooling conditions, remove the obvious bone marrow tissue, and store them in liquid nitrogen for later use;

[0057] B: Put a certain size of bone into 200mL of 0.5 M EDTA (pH 8.0) and 0.25% TritonX-100 mixed solution, decalcify for about 1 week under constant stirring at room temperature, until the bone becomes soft and can be easily pierced with a pin enter. The solution was changed once a day;

[0058] C: The demineralized bone slices treated in B were frozen in a -20°C refrigerator, and then cut into membranes about 200 μm thick with a frozen tissue slicer;

[0059] D: Immerse the demineralized bone treated in step C in PBS solution of 0.25% TritonX-1...

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Abstract

The invention provides a method for preparing an absorbable and antibacterial guided tissue regeneration membrane for a bone-like structure, and belongs to the field of biological medical materials. The method comprises the following steps: firstly, preparing a cell free bovine pericardial collagen fiber membrane by adopting a repeated freeze-thaw method and a surface active agent TritonX-100, or preparing a demineralized cell free collagen fiber membrane of a cattle lamellar bone by using a method of EDTA decalcification by using the surfactant TritonX-100; treating by using sodium trimetaphosphate to obtain a phosphorylated collagen fiber membrane; with the assistance of a direct-current electric field, mineralizing in agar hydrogel containing calcium and phosphate, thereby obtaining a mineralized collagen membrane with a bone-like structure with certain hardness; and finally soaking the mineralized collagen membrane into a minocyline solution, and preparing an antibacterial bone-like structured composite membrane structure by virtue of the characteristics of combined minocyline and hydroxyapatite. The guided tissue regeneration membrane provided by the invention has a bone-like structure, is capable of maintaining a bone defect space, is good in operation forming property and antibacterial property, and can be applied to clinical practice for bone defect remediation and reconstruction in periodontal departments, dental implant mediation and occlusalf surfaces.

Description

technical field [0001] The invention belongs to biomedical material technology, and relates to a preparation method of an absorbable film material. It is used to guide the regeneration of bone tissue and periodontal tissue in clinical oral cavity, and to regenerate bone tissue in repair and reconstruction surgery. Background technique [0002] Membrane-guided bone regeneration (GBR): refers to a new technology that establishes a physical barrier in the bone defect area to isolate the bone defect area from the surrounding soft tissue that interferes with bone growth, so as to promote new bone growth. In 1957, "Americal Journal of Surgery" reported for the first time: a hard plastic was covered on the experimental defect of the dog's tibia. After 10 weeks, new bone was found to grow into the bone wound covered by the hard plastic. The amount of bone growth was determined by the membrane. The size of the space under the object. Therefore, it is considered that preventing soft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/02A61L31/16
Inventor 李柏霖李全利
Owner 李柏霖
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