Culture medium for producing virginiamycin through streptomyces virginiae fermentation and feeding method of culture medium

A technology of virginiamycin and Streptomyces virginica, applied in the field of fermentation, can solve the problems of affecting the quality of fermentation broth and fermentation effect, high production cost of virginiamycin, high viscosity of fermentation broth, etc. The effect of quality and fermentation technology level, ease of procurement, and low fermentation cost

Active Publication Date: 2015-04-01
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 The fermentation unit is maintained at around 1000-3000u/ml, resulting in a low fermentation yield
[0005] 2 The quality of animal nitrogen source affects the quality of fermentation broth and fermentation effect
As a result, there are more remaining proteins in

Method used

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  • Culture medium for producing virginiamycin through streptomyces virginiae fermentation and feeding method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1m 3 Seed Pot Culture:

[0051] 20L of maltose, 10kg of mixed starch, 1L of olive oil, 15kg of low-temperature pressed soybean cake powder, 20kg of rice distiller's grains, 10kg of corn gluten powder, 1kg of light calcium carbonate, 1kg of ammonium sulfate, and 10g of maltase.

[0052] During the seed cultivation process, the tank pressure is 0.02-0.04MPa; the tank temperature is 25-28°C; the air flow rate is 80m 3 / h; stirring speed 60~80r / min; pH6~8; culture time 32h. After the seed culture is over, the cell concentration is 21%, and there is no other miscellaneous bacteria pollution.

[0053] 10m 3 Fermenter culture:

[0054] 300L of maltose, 200kg of mixed starch, 10L of olive oil, 200kg of low-temperature pressed soybean cake powder, 300kg of rice distiller’s grains, 200kg of corn gluten powder, 8kg of potassium dihydrogen phosphate, 60kg of light calcium carbonate, 50kg of ammonium sulfate, 5kg of sodium acetate, and magnesium sulfate 10kg, ferrous sulfate 3kg,...

Embodiment 2

[0058] 1m 3 Seed Pot Culture:

[0059] 30L of maltose, 13kg of mixed starch, 2L of olive oil, 17kg of low-temperature pressed soybean cake powder, 23kg of rice distiller's grains, 11kg of corn gluten powder, 2kg of light calcium carbonate, 2kg of ammonium sulfate, and 30g of maltase.

[0060] During the seed cultivation process, the tank pressure is 0.02-0.04MPa; the tank temperature is 25-28°C; the air flow rate is 80m 3 / h; stirring speed 60~80r / min; pH6~8; culture time 33h. After the seed culture is over, the cell concentration is 25%, and there is no other miscellaneous bacteria pollution.

[0061] 10m 3 Fermenter culture:

[0062] 330L of maltose, 400kg of mixed starch, 20L of olive oil, 220kg of low-temperature pressed soybean cake powder, 330kg of rice distiller’s grains, 220kg of corn gluten powder, 9kg of potassium dihydrogen phosphate, 60kg of light calcium carbonate, 60kg of ammonium sulfate, 7kg of sodium acetate, and magnesium sulfate 20kg, ferrous sulfate 4k...

Embodiment 3

[0066] 1m 3 Seed Pot Culture:

[0067] 25L of maltose, 15kg of mixed starch, 3L of olive oil, 15kg of low-temperature pressed soybean cake powder, 25kg of rice distiller's grains, 15kg of corn gluten powder, 3kg of light calcium carbonate, 3kg of ammonium sulfate, and 20g of maltase.

[0068] During the seed cultivation process, the tank pressure is 0.02-0.04MPa; the tank temperature is 25-28°C; the air flow rate is 80m 3 / h; stirring speed 60~80r / min; pH6~8; culture time 34h. After the seed culture is over, the cell concentration is 25%, and there is no other miscellaneous bacteria pollution.

[0069] 10m 3 Fermenter culture:

[0070] 350L of maltose, 200kg of mixed starch, 30L of olive oil, 250kg of low-temperature pressed soybean cake powder, 400kg of rice distiller’s grains, 250kg of corn gluten powder, 10kg of potassium dihydrogen phosphate, 70kg of light calcium carbonate, 65kg of ammonium sulfate, 10kg of sodium acetate, and magnesium sulfate 30kg, ferrous sulfate ...

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Abstract

The invention relates to a culture medium for producing virginiamycin through streptomyces virginiae fermentation and a feeding method of the culture medium. A seed culture medium comprises rice vinasse and low-temperature pressed soybean cake powder; a fermentation culture medium comprises rice vinasse, low-temperature pressed soybean cake powder, Dow macroporous ion exchange resin and a nonionic surfactant. Through the adoption of the culture medium and the feeding method, the cost problem of raw and supplemental materials is solved, the environment influence on the sources of the raw and supplemental materials is reduced to the greatest degree, the sources of the raw and supplemental materials are guaranteed to be fed sufficiently, the stable and efficient production of the virginiamycin is achieved, and meanwhile, the fermentation unit can be increased by using the culture medium and a culturing method.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and feeding method for virginiamycin fermented by Streptomyces virginiae. Background technique [0002] Virginiamycin is also known as virginiamycin, virginiamycin, weilimycin, mast and so on. Structurally, it is classified as a chain-positive antibiotic, and it is a kind of veterinary antibiotic with a relatively novel structure. [0003] At present, the domestic use of Streptomyces virginiae to produce virginiamycin by fermentation, the main problems are: [0004] 1 The fermentation unit is maintained at about 1000-3000u / ml, resulting in a low fermentation yield. [0005] 2 The quality of animal nitrogen source affects the quality of fermentation broth and fermentation effect. At present, fish meal or peptone or yeast extract or two or more nitrogen sources are added to the culture medium for domestic fermentative production of virginiamycin...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12R1/465
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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