Culture medium for producing virginiamycin through streptomyces virginiae fermentation and feeding method of culture medium

Abstract

The invention relates to a culture medium for producing virginiamycin through streptomyces virginiae fermentation and a feeding method of the culture medium. A seed culture medium comprises rice vinasse and low-temperature pressed soybean cake powder; a fermentation culture medium comprises rice vinasse, low-temperature pressed soybean cake powder, Dow macroporous ion exchange resin and a nonionic surfactant. Through the adoption of the culture medium and the feeding method, the cost problem of raw and supplemental materials is solved, the environment influence on the sources of the raw and supplemental materials is reduced to the greatest degree, the sources of the raw and supplemental materials are guaranteed to be fed sufficiently, the stable and efficient production of the virginiamycin is achieved, and meanwhile, the fermentation unit can be increased by using the culture medium and a culturing method.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and feeding method for virginiamycin fermented by Streptomyces virginiae. Background technique [0002] Virginiamycin is also known as virginiamycin, virginiamycin, weilimycin, mast and so on. Structurally, it is classified as a chain-positive antibiotic, and it is a kind of veterinary antibiotic with a relatively novel structure. [0003] At present, the domestic use of Streptomyces virginiae to produce virginiamycin by fermentation, the main problems are: [0004] 1 The fermentation unit is maintained at about 1000-3000u / ml, resulting in a low fermentation yield. [0005] 2 The quality of animal nitrogen source affects the quality of fermentation broth and fermentation effect. At present, fish meal or peptone or yeast extract or two or more nitrogen sources are added to the culture medium for domestic fermentative production of virginiamycin...

AI Technical Summary

Problems solved by technology

[0004] 1 The fermentation unit is maintained at around 1000-3000u / ml, resulting in a low fermentation yield

[0005] 2 The quality of animal nitrogen source affects the quality of fermentation broth and fermentation effect

As a result, there are more remaining proteins in the late stage of fermentation, and the viscosity of the fermentation broth is higher, which reduces the extraction yield.

[0006] 3 The production cost of virginiamycin is relatively high, and the cost of nitrogen source accounts for about 40% of the fermentation cost

Images