Preparation method of mash

A technology for mash and enzymatically hydrolyzed sugar solution, which is applied in the directions of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of incomplete reaction, long production cycle, reduced production efficiency, etc. The effect of eliminating feedback inhibition, reducing sugar liquid loss and improving production efficiency

Inactive Publication Date: 2015-04-08
SONGYUAN GUANGHE ENERGY
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Problems solved by technology

[0007] In the existing production method of mash, enzymolysis and fermentation are carried out in two stages, and the enzymolysis reaction reduces the reaction speed due to the inhibitory effect of the product, and the reaction is incomplete, which reduces the enzymolysis efficiency; and the production cycle is long, Reduced production efficiency; enzymatic sugar solution needs to be detoxified by solid-liquid separator, the process is complicated, and part of the sugar solution is lost

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preparation example Construction

[0028] The embodiment of the invention discloses a method for preparing mash, comprising the following steps:

[0029] (A) Pretreating the crop stalks through steam explosion;

[0030] (B) adding cellulase and domesticated Saccharomyces cerevisiae to the pretreated product for enzymolysis and fermentation to obtain mash;

[0031] The domestication method of described saccharomyces cerevisiae is:

[0032] The activated Saccharomyces cerevisiae is cultured in the mixture of corn mash and enzymatic sugar liquid to obtain small wine mother; the mass ratio of the corn mash and enzymatic sugar liquid is (80-90): (10-20 );

[0033] The small wine tree is subjected to multi-generation expansion culture in the mixture of corn mash and enzymatic sugar liquid to obtain Dajiu Fu; in the first generation of expanded culture, the mass ratio of corn mash and enzymatic sugar liquid is (60~ 70): (30-40); in each subsequent generation of expanded culture, gradually increase the proportion of...

Embodiment 1

[0052] (1) Domestication of Saccharomyces cerevisiae:

[0053] Prepare activation medium: mix 20 ml of 2% glucose, 0.375 g of diammonium phosphate, 0.375 g of urea, and 0.25 g of magnesium sulfate, and add water to 500 ml.

[0054] Preparation of enzymatic sugar solution: maintain 1000 kg of crop stalks at a steam pressure of 1.4 MPa for 10 minutes to obtain a pretreated product; quickly depressurize to obtain a steam explosion pretreated product; % Add cellulase, carry out enzymatic hydrolysis under the conditions of normal pressure, 45° C., and pH 4.8, to obtain enzymatic hydrolyzed sugar solution.

[0055] Add 0.5 g of dry yeast to the activation medium, shake well, and activate in a 38° C. water bath for 1 hour.

[0056] Add 20 grams of the activated yeast to 90 ml of corn mash and 10 ml of enzymatic sugar solution, place in a water bath at 30° C., ventilate and cultivate for 10 hours to obtain Xiaojiufu.

[0057] Add 150ml of mature xiaojiufu to the mixture of 240ml cor...

Embodiment 2

[0065] (1) Domestication of Saccharomyces cerevisiae:

[0066] Prepare activation medium: mix 20 ml of 2% glucose, 0.375 g of diammonium phosphate, 0.375 g of urea, and 0.25 g of magnesium sulfate, and add water to 500 ml.

[0067] Preparation of enzymatic sugar solution: maintain 1,000 kg of crop straw at a steam pressure of 1.5 MPa for 12 minutes to obtain a pretreated product; quickly depressurize to obtain a steam explosion pretreated product; % Add cellulase, carry out enzymatic hydrolysis under the conditions of normal pressure, 45° C., and pH 4.8, to obtain enzymatic hydrolyzed sugar solution.

[0068] Add 0.5 g of dry yeast Saccharomyces cerevisiae to the activation medium, shake well, and activate in a water bath at 36° C. for 1.2 hours.

[0069] Add 20 grams of the activated yeast to 85 ml of corn mash and 15 ml of enzymatic sugar solution, adjust the pH to 4.5, place it in a water bath at 28° C., and cultivate it with ventilation for 12 hours to obtain Xiaojiufu. ...

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Abstract

The invention provides a preparation method of mash. The method comprises the following steps: carrying out steam explosion pretreatment on crop straws; adding cellulase and domesticated saccharomyces cerevisiae to the pretreated product, and carrying out enzymolysis and fermentation, so as to obtain the mash. Firstly, steam explosion pretreatment is adopted, no chemical substance is added, and detoxification treatment is not needed, so that the flow is simplified; secondly, the domesticated saccharomyces cerevisiae is adopted to ferment, and the domesticated saccharomyces cerevisiae has relatively good adaptive capacity on inhibitors generated by fermentation, so that the fermentation effect is good; and thirdly, enzymolysis and fermentation are synchronously carried out, and the glucose generated by enzymolysis is rapidly metabolized to generate ethanol, so that feedback inhibition action on the cellulase caused by increase of the concentration of substrate glucose is removed, the flow is simplified, the loss of liquid glucose is reduced, and the production efficiency is improved.

Description

technical field [0001] The invention relates to the field of cellulose ethanol production, in particular to a preparation method of mash. Background technique [0002] With the gradual depletion of oil resources and the deterioration of the environment, vigorously promoting the use of renewable energy technologies has become an important part of many countries' energy development strategies to reduce dependence on oil resources and greenhouse gas emissions. [0003] Cellulosic ethanol technology is a high-end clean energy technology, because it can be used to replace traditional grain ethanol technology, using cellulosic biomass widely existing on the earth to produce clean ethanol fuel, has high expectations . [0004] Cellulosic ethanol refers to ethanol produced from cellulosic biomass. Cellulosic biomass is a complex material composed of cellulose (30-50%), hemicellulose (20-40%), and lignin (15-30%), which can be crop straw, forestry processing waste, sugarcane slag ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12N1/36C12N1/18C12R1/865
CPCC12N1/36C12P7/10C12P19/14Y02E50/10
Inventor 生晓东刘玉金肖伟良李学业
Owner SONGYUAN GUANGHE ENERGY
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