Fucan sulfate and application of low-molecular-weight fucan sulfate in preparation of blood fat reduction and weight loss drugs and health care products

A technology of fucoidan sulfate and low molecular weight, applied in the field of medicine, can solve the problems of no fucoidan sulfate, weight loss, etc., and achieves reducing blood lipid metabolism disorder, reducing weight and abdominal fat content, and reducing weight The effect of raising symptoms

Inactive Publication Date: 2015-04-22
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the application of fucoidan sulfa...

Method used

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  • Fucan sulfate and application of low-molecular-weight fucan sulfate in preparation of blood fat reduction and weight loss drugs and health care products
  • Fucan sulfate and application of low-molecular-weight fucan sulfate in preparation of blood fat reduction and weight loss drugs and health care products
  • Fucan sulfate and application of low-molecular-weight fucan sulfate in preparation of blood fat reduction and weight loss drugs and health care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of fucoidan sulfate ester of the present invention comprises the following steps:

[0025] (1) Put the brown algae powder in an organic solvent such as methanol, acetone or ethanol with a mass ratio of 80%, soak each time at 80°C for 3 hours, repeat 3 times, to degrease by soaking;

[0026] The brown algae is taken from one or more of Fucus, Ascophyllum nodosum, Sargasso, Undaria pinnatifida, Hijiki, Sage, sea millet, kelp or kelp, among which Fucus is preferred.

[0027] (2) Then extract with water at 80°C, then precipitate with ethanol with a mass ratio of 80%, and then dry at 40°C to obtain crude fucoidan sulfate;

[0028] (3) Then in CaCl 2 or MgCl 2 Precipitation with 20% ethanol in the presence of water-soluble alginate;

[0029] (4) through dialysis and concentration again, be 80% ethanol precipitation with mass ratio, then obtain purified fucoidan sulfate after drying at 40°C; the molecular weight of the prepared fucoidan sulfate is 200...

Embodiment 2

[0031] Example 2, Cytotoxicity Detection of Fucoidan Sulfate (FS) and Low Molecular Weight Fucoidan Sulfate (LFS)

[0032] The present invention adopts a classic cell model of lipid metabolism disorder and an animal model of hyperlipidemia and obesity to evaluate the toxicity of the fucoidan sulfate at the cell and animal levels, and the effect of lowering blood fat and body weight. Depend on figure 1 It can be seen that fucoidan sulfate (FS) and low molecular weight fucoidan sulfate (LFS) have no obvious toxicity to cells in the range of 0-400 μg / mL, and when the concentration reaches 500 μg / mL and above, the exhibited some cytotoxicity. However, the highest experimental concentration of the present invention is 100 μg / mL, so it will not produce any toxicity to cells.

Embodiment 3

[0033] Embodiment 3, fucoidan sulfate (FS) and low molecular weight fucoidan sulfate (LFS) reduce triglyceride and cholesterol in vitro

[0034] The inhibitory effect of FS and LFS on triglyceride and cholesterol was evaluated by using the cell model established by the human liver cell line HepG2 exposed to high-fat and fat-inducing factor medium. The positive drugs were simvastatin and metformin.

[0035] The experimental results are shown in Table 1. FS and LFS can significantly reduce the content of triglyceride and cholesterol, and compared with the positive drug simvastatin, the effect of lowering triglyceride is more obvious; Cholesterol works better too. It shows that FS and LFS have a better effect of lowering triglyceride and cholesterol than simvastatin and metformin, which are commonly used in clinical practice.

[0036] Table 1 Effects of FS and LFS on TG and TC of hepatocytes

[0037]

[0038] *P<0.05, compared with the control model group;

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Abstract

The invention provides fucan sulfate and application of low-molecular-weight fucan sulfate in preparation of blood fat reduction and weight loss drugs and health care products. The fucan sulfate and the low-molecular-weight fucan sulfate can be used for remarkably suppressing aggregation of triglyceride and cholesterol of liver cells when concentration is larger than 50 micrograms per milliliter, and the fucan sulfate and the low-molecular-weight fucan sulfate can be used for effectively reducing the level of TG, TC and LDL in blood, increasing the level of HDL in the blood and obviously reducing weight and the content of abdominal fat. As is provided through experiments, FS and LFS have a good function of reducing blood fat and losing weight. The raw materials come from alga and have the advantages of being rich in resource, low in cost, high in safety performance and the like, the situation that the high blood fat and obesity state can be remarkably improved is proved both in a body and outside the body, and a new way is provided for developing the kind of drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of fucoidan sulfate and low-molecular weight fucoidan sulfate in the preparation of medicines and health care products for reducing blood fat and weight loss. Background technique [0002] Hyperlipidemia is mainly caused by the increase of plasma cholesterol (cholesterol, TC), triglyceride (triglyceride, TG), low density lipoprotein (low density lipoprotein, LDL), high density lipoprotein (high density lipoprotein, HDL) It is one of the main inducing factors of diseases such as atherosclerosis, heart disease, stroke and fatty liver, which seriously threatens people's health. Effectively controlling hyperlipidemia is the key to preventing cardiovascular and cerebrovascular diseases. With the aging of the population and the increase in fat intake in people's diet and the decrease in exercise, the incidence of hyperlipidemia and its complications is incr...

Claims

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Application Information

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IPC IPC(8): A61K31/737C08B37/00A61P3/06A61P3/04A23L1/30A23L33/10
Inventor 郝杰杰管华诗敦云楼周晓琳于广利夏萱
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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