Polyamino acidic elastic vascular tissue engineering stent and preparation method thereof

Abstract

The invention discloses a polyamino acidic elastic vascular tissue engineering stent and a preparation method thereof; an inner layer is formed by compounding -NH<3+> in polysaccharide with -COO- in polyamino acid in an electrostatic compounding manner; an intermediate layer is a chemical crosslinking stent layer; an outer layer is an electrostatic spinning net; and the electrostatic spinning net is prepared from a poly(epsilon-caprolactone)-b-polyamino acid ester segmented copolymer through an electrostatic spinning method. According to the invention, the complex structure and the mechanical property of normal vessels are simulated; on one hand, the stent has good biocompatibility; the compliance of the stent can be well matched with a host vessel; on the other hand, the intermediate layer of stent has relatively good elasticity; under the dynamic stimulation of a vascular bioreactor, the stent can better bear wall-crossing pressure and resilience of a blood flow to a vascular wall; furthermore, the stent is deformed periodically, so that self remodelling can be strengthened; therefore, a regenerated blood vessel is closer to a normal blood vessel in tissue constitution; and furthermore, the polyamino acidic elastic vascular tissue engineering stent has excellent biomechanical property.

Description

technical field [0001] The invention relates to a polyamino acid-based elastic blood vessel tissue engineering scaffold and a preparation method thereof. Background technique [0002] Cardiovascular disease is recognized as the main cause of morbidity and death. The World Health Organization announced in September 2011 that the proportion of death caused by cardiovascular disease was 30%. In the United States alone, 1 million people die from cardiovascular disease each year. Treatments for this disease include chemotherapy, angioplasty, stent insertion, resection of diseased tissue, and others. However, for patients, the success rate of this type of treatment is low, and there is a status quo of disease progression. At present, the most commonly used method is the reconstruction and replacement of blood vessels. However, due to the limitation of the source of autologous and allogeneic blood vessels, uncontrollable physical and chemical properties of tissues, severe inflam...

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Problems solved by technology

However, such materials still cannot avoid degradation products that are acidic, immunogenic, and cause thromboembolism, and the regenerated vascular tissue lacks mechanical properties.

[0006] The fourth is to plant cells on biodegradable scaffolds for in vitro vascular regeneration. Although thromboembolism can be effectively avoided, the regenerated blood vessels lack mechanical strength.

Biomaterials (vol.31, 2010, p621-630) disclosed that under dynamic / static stimulation, smooth muscle cells were planted on non-woven polyglycolide porous scaffolds to successfully regenerate the vascular smooth muscle layer. Through histological and biomechanical analysis, dynamic stimulation The structure of the regenerated vascular smooth muscle layer is more similar to that of the normal vascular smooth muscle layer, but the material lacks elasticity

Therefore, it is still a huge challenge to prepare regenerated blood vessels similar to normal blood vessels. The key technology is due to the fact that the mechanical properties of scaffold materials cannot meet clinical needs.

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