Polyamino acidic elastic vascular tissue engineering stent and preparation method thereof

A polyamino acid and vascular tissue technology, which is applied in non-woven fabrics, textiles and papermaking, medical science and other directions, can solve the problems that the mechanical properties of scaffold materials cannot meet clinical needs, the lack of mechanical strength of regenerated blood vessels, and the lack of mechanical properties of vascular tissue, etc. Achieve excellent biomechanical strength, good biocompatibility, and low biodegradability

Inactive Publication Date: 2015-04-22
SHANGHAI UNIV
View PDF7 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such materials still cannot avoid degradation products that are acidic, immunogenic, and cause thromboembolism, and the regenerated vascular tissue lacks mechanical properties.
[0006] The fourth is to plant cells on biodegradable scaffolds for in vitro vascular regeneration. Although thromboembolism can be effectively avoided, the regenerated blood vessels lack mechanical strength.
Biomaterials (vol.31, 2010, p621-630) disclosed that under dynamic/static stimulation, smooth muscle cells were planted on non-woven polyglycolide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polyamino acidic elastic vascular tissue engineering stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] The purpose of the present invention is to provide a vascular tissue engineering scaffold material and a preparation method thereof, which can simulate normal blood vessels in terms of structure and mechanical properties. Include the following steps:

[0034] A) an inner layer, the inner layer is made of an electrostatic composite scaffold, and the electrostatic composite scaffold is electrostatically blended by polyamino acids and polysaccharides;

[0035] B) Middle layer made of chemically cross-linked gel scaffold based on poly((ε-caprolactone)- b -(ethylene glycol)- b -(ε-caprolactone)) tri-block copolymer is obtained by cross-linking polyamino acid to obtain polyamino acid / poly((ε-caprolactone)- b -(ethylene glycol)- b -(ε-caprolactone)) composite gel, through gradient dialysis and freeze-drying to obtain a porous scaffold;

[0036] C) an outer layer made of an electrospun mesh made of poly(ε-caprolactone)- b - Made of polyamino acid ester block copolymer.

...

Embodiment 1

[0046] The present embodiment provides the construction of vascular stent, comprising the following steps:

[0047] Vascular stent A) Construction of the inner layer: respectively prepare poly(L-glutamic acid) and chitosan aqueous solutions with a molar ratio of carboxyl and amino groups of 1:0.5, adjust the pH value to between the pKa values ​​of the two, and obtain after blending The electrostatic composite floc solution is poured into a pre-prepared mold and freeze-dried to obtain a hollow porous inner layer support. The scaffold material exhibited a sponge-like morphology. The pore size distribution is 100-150 mm; the porosity is above 85%.

[0048] Vascular stent B) construction of the middle layer: prepare an elastic stent with a cross-linking degree of 50% in the middle layer. First, the poly((ε-caprolactone)- b -(ethylene glycol)- b -(ε-caprolactone)) (Mn: 1000-400-1000) triblock copolymer and poly(L-glutamic acid) (Mn: 100000) DMSO / DMF solution, followed by additi...

Embodiment 2

[0051] Vascular stent A) Construction of the inner layer: respectively prepare poly(L-glutamic acid) and chitosan aqueous solutions with a molar ratio of carboxyl and amino groups of 1:1, adjust the pH value to between the pKa values ​​of the two, and obtain after blending The electrostatic composite floc solution is poured into a pre-prepared mold and freeze-dried to obtain a hollow porous inner layer support. The scaffold material exhibited a sponge-like morphology. The pore size distribution is 100-150 mm; the porosity is above 85%.

[0052] Vascular stent B) construction of the middle layer: prepare an elastic stent with a cross-linking degree of 50% in the middle layer. First, the poly((ε-caprolactone)- b -(ethylene glycol)- b -(ε-caprolactone)) (Mn: 1000-400-1000) triblock copolymer and poly(L-glutamic acid) (Mn: 100000) DMSO / DMF solution, followed by addition of catalyst, its EDC HCl: The molar ratio of DMAP is 1:0.1, stir to make it evenly dispersed, and pour the s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Lengthaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a polyamino acidic elastic vascular tissue engineering stent and a preparation method thereof; an inner layer is formed by compounding -NH<3+> in polysaccharide with -COO- in polyamino acid in an electrostatic compounding manner; an intermediate layer is a chemical crosslinking stent layer; an outer layer is an electrostatic spinning net; and the electrostatic spinning net is prepared from a poly(epsilon-caprolactone)-b-polyamino acid ester segmented copolymer through an electrostatic spinning method. According to the invention, the complex structure and the mechanical property of normal vessels are simulated; on one hand, the stent has good biocompatibility; the compliance of the stent can be well matched with a host vessel; on the other hand, the intermediate layer of stent has relatively good elasticity; under the dynamic stimulation of a vascular bioreactor, the stent can better bear wall-crossing pressure and resilience of a blood flow to a vascular wall; furthermore, the stent is deformed periodically, so that self remodelling can be strengthened; therefore, a regenerated blood vessel is closer to a normal blood vessel in tissue constitution; and furthermore, the polyamino acidic elastic vascular tissue engineering stent has excellent biomechanical property.

Description

technical field [0001] The invention relates to a polyamino acid-based elastic blood vessel tissue engineering scaffold and a preparation method thereof. Background technique [0002] Cardiovascular disease is recognized as the main cause of morbidity and death. The World Health Organization announced in September 2011 that the proportion of death caused by cardiovascular disease was 30%. In the United States alone, 1 million people die from cardiovascular disease each year. Treatments for this disease include chemotherapy, angioplasty, stent insertion, resection of diseased tissue, and others. However, for patients, the success rate of this type of treatment is low, and there is a status quo of disease progression. At present, the most commonly used method is the reconstruction and replacement of blood vessels. However, due to the limitation of the source of autologous and allogeneic blood vessels, uncontrollable physical and chemical properties of tissues, severe inflam...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L31/04A61L31/14D04H1/70D04H1/76D04H3/005D04H3/009
Inventor 尹静波吴杰张坤玺张伟骏
Owner SHANGHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap