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Tissue culture solution for dendrobium candidum

A technology of tissue culture and dendrobium candidum, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of low yield and scale, low emergence rate, low survival rate, etc., to improve energy supply, Effects of increasing rooting rate and growth rate and improving survival rate

Active Publication Date: 2015-04-29
太湖县皖松工艺品有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the artificial cultivation of Dendrobium officinale has been successful, the cycle is long, the survival rate is low, and the emergence rate is low, resulting in the production and scale not being improved, and it is difficult to meet the needs of social applications.

Method used

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  • Tissue culture solution for dendrobium candidum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A kind of dendrobium candidum tissue culture liquid that the present invention proposes, its raw material comprises by volume percentage: 0.4% first mother liquor, 0.7% second mother liquor, 0.4% third mother liquor and 98.5% fourth mother liquor, first mother liquor, second mother liquor 1. Both the third mother liquor and the fourth mother liquor are aqueous solutions; the components of the first mother liquor include: potassium iodide 0.1g / L, manganese sulfate 3g / L, zinc sulfate 1g / L, boric acid 2g / L, sodium molybdate 0.05g / L , copper sulfate 0.012g / L, cobalt chloride 0.01g / L; the components of the second mother liquor include: ferrous sulfate 0.012mol / L, disodium edetate 0.01mol / L; the raw materials of the third mother liquor according to Parts by weight include: Inositol 8 x 10 3 12 parts of niacin, 35 parts of pyridoxine hydrochloride, 3 parts of thiamine hydrochloride, 65 parts of glycine, 1.5×10 distilled water 5 Parts; the components of the fourth mother liquo...

Embodiment 2

[0016] A kind of dendrobium candidum tissue culture liquid that the present invention proposes, its raw material comprises by volume percentage: 0.6% first mother liquor, 0.4% second mother liquor, 0.6% third mother liquor and 98.4% fourth mother liquor, first mother liquor, second mother liquor 1. Both the third mother liquor and the fourth mother liquor are aqueous solutions; the components of the first mother liquor include: potassium iodide 0.2g / L, manganese sulfate 2g / L, zinc sulfate 2g / L, boric acid 1g / L, sodium molybdate 0.15g / L , copper sulfate 0.008g / L, cobalt chloride 0.03g / L; the components of the second mother liquor include: ferrous sulfate 0.008mol / L, disodium edetate 0.05mol / L; the raw materials of the third mother liquor according to Parts by weight include: Inositol 4 x 10 3 17 parts of niacin, 25 parts of pyridoxine hydrochloride, 9 parts of thiamine hydrochloride, 55 parts of glycine, 2.5×10 distilled water 5 Part; the component of the 4th mother liquor com...

Embodiment 3

[0018] A kind of Dendrobium officinale tissue culture liquid proposed by the present invention, its raw material comprises by volume percentage: 0.42% first mother liquor, 0.63% second mother liquor, 0.41% third mother liquor and 98.54% fourth mother liquor, first mother liquor, second mother liquor , the third mother liquor and the fourth mother liquor are both aqueous solutions; the components of the first mother liquor include: potassium iodide 0.13g / L, manganese sulfate 2.5g / L, zinc sulfate 1.3g / L, boric acid 1.9g / L, sodium molybdate 0.08 g / L, copper sulfate 0.011g / L, cobalt chloride 0.015g / L; the components of the second mother liquor include: ferrous sulfate 0.011mol / L, disodium edetate 0.02mol / L; the third mother liquor The raw materials include by weight: inositol 7×10 3 14 parts of niacin, 33 parts of pyridoxine hydrochloride, 5 parts of thiamine hydrochloride, 62 parts of glycine, 1.8×10 distilled water 5 part; the components of the fourth mother liquor include: ban...

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Abstract

The invention discloses a tissue culture solution for the dendrobium candidum. The tissue culture solution for the dendrobium candidum comprises the following raw materials in percent by volume: 0.4-0.6% of a first mother solution, 0.4-0.7% of a second mother solution, 0.4-0.6% of a third mother solution, and 98-99% of a fourth mother solution. The first mother solution comprises the following components: 0.1-0.2 g / L potassium iodide, 2-3 g / L manganese sulfate, 1-2 g / L zinc sulfate, 1-2 g / L boric acid, 0.05-0.15 g / L sodium molybdate, 0.008-0.012 g / L copper sulfate, and 0.01-0.03 g / L of cobalt chloride; the fourth mother solution comprises the following components: 70-72 g / L of mashed banana, 0.7-1 g / L of ammonium nitrate, 0.8-1.2 g / L of potassium nitrate, 0.2-0.3 g / L of calcium chloride, 0.15-0.2 g / L of magnesium sulfate, 0.05-0.13 g / L of monocalcium phosphate, 39-42 g / L of white sugar and 3.8-4.3 g / L of agar.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a tissue culture solution of Dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of Orchidaceae. Born on the semi-shady rocks in the mountains at an altitude of 1600 meters, it likes warm and humid climates and semi-shady and semi-yang environments, and is not cold-resistant. Generally, they can withstand low temperatures of -5°C. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum is the best product of Dendrobium. It is named for its iron-green skin. Dendrobium candidum has unique medicinal value, and its stem is used as medicine. The name of the traditional Chinese medicine: Dendrobium, belongs to the yin-tonifying medicine in the tonic medicine. "Chinese Pharmacopoeia": Yiwei Shengjin, nourishing yin and clearing heat. Due to over-excavation and the destructio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李军
Owner 太湖县皖松工艺品有限责任公司
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