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A kind of ferrotrophic bacteria and its method for removing heavy metals in soil

A technology of ferric bacteria and heavy metals, applied in the field of environmental science and geotechnical engineering, can solve the problems of plants being limited by regional influence, difficult to guarantee the treatment effect, and lengthy process flow, so as to ensure ecological health and environment, and ensure sustainable development. , the effect of low investment cost

Active Publication Date: 2017-07-11
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chemical repair methods include chemical precipitation, ion exchange, solvent extraction, etc. These methods can achieve results under certain conditions, but generally have the disadvantages of lengthy process, high cost of raw materials, and easy to cause secondary pollution.
Phytoremediation mainly includes plant extraction, plant fixation, plant volatilization, plant filtration, etc. However, this method has disadvantages such as small depth of impact, long repair period, limited plants affected by the area, and difficult to guarantee the treatment effect, so the applicable conditions are limited.

Method used

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  • A kind of ferrotrophic bacteria and its method for removing heavy metals in soil
  • A kind of ferrotrophic bacteria and its method for removing heavy metals in soil
  • A kind of ferrotrophic bacteria and its method for removing heavy metals in soil

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Effect test

Embodiment 1

[0035] Embodiment 1: the acquisition of S1969 bacterial strain

[0036] Collect the soil, dissolve it with pure water, dilute it and spread it on the solid medium of ferrotrophic bacteria, culture it in a 28°C incubator for 3-7 days, pick a single colony according to the shape and color of the colony, and save it after purification. The screened strain code-named S1969 has a strong metabolism, relatively stable individual morphology and physiological characteristics, produces more red slime, and can tolerate higher concentrations of heavy metal ions. like figure 1 and figure 2 as shown, figure 1 lead to Fe for strain metabolites 2+ is oxidized, eventually forming a picture of iron-based complex deposits; figure 2 The pictures before and after the co-precipitation of iron-based complexes and heavy metals were caused by the metabolites of the strain.

Embodiment 2

[0037] Embodiment 2: Identification and preservation of the S1969 strain

[0038] According to Sangong SK1201, the DNA of strain S1969 was extracted and the 16SrDNA sequence was amplified. Bacterial universal primers 7F (5'-CAGAGTTTGATCCTGGCTCAG-3', SEQ ID NO.1) and 154OR (5'-AGGAGGTGATCCAGCCGCA-3', SEQ ID NO.2) were used for amplification. The amplified product was recovered and purified and sent to Shanghai Sangon Bioengineering Co., Ltd. for sequencing. The sequencing results were compared with the known sequences in the rep database to determine the taxonomic status of the strain.

[0039] The colony of the strain S1969 is red, with rough surface and irregular edges. It is a Gram-positive bacterium. It is suitable for growth in the environment of pH=6.8-7 and culture temperature of about 30°C.

[0040]The strain S1969 was preserved on November 29, 2013 in the General Microbiology Center of the China Microbiological Culture Collection Management Committee (No. 3, No. 1, Be...

Embodiment 3

[0041] Embodiment 3: the preparation of bacterial liquid

[0042] Bacterial solution: Each liter of culture solution contains 10g of ferric ammonium citrate, 0.5g of magnesium sulfate containing crystal water, 0.5g of ferrous ammonium sulfate, 0.5g of dipotassium hydrogen phosphate, 0.2g of calcium chloride, 0.5g of sodium nitrate, pH The value was controlled at 6.8-7, and 100mL of culture solution was put into a 300mL small-mouth Erlenmeyer flask, and sterilized at 121°C for 30min. Inoculate S1969 in the culture medium, cultivate it at 30°C with a rotation speed of 200r / min, and obtain the bacterial fluid after culturing for 48 hours.

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Abstract

The invention relates to a ferrotroph and a method for using it to remove heavy metals in soil. The invention relates to ferrotrophic bacteria S1969 which is red rod-shaped, has rough surface and irregular edges, and is a gram-positive bacterium. It prepares standard culture solution, bacteria solution and absorbs heavy metals in soil. The invention overcomes the respective defects of the conventional remediation methods of soil heavy metal pollution, such as physical remediation, chemical remediation, phytoremediation and microbial remediation. The S1969 bacterial strain of the present invention is derived from domestic general soil. The microorganisms isolated and screened from the soil have strong adaptability, can remove heavy metals, and are rich in sources and easy to cultivate. , Cu2+, Zn2+ and other heavy metal ions have significantly reduced bioavailability. Therefore, the adsorption and precipitation of heavy metals by S1969 bacterial solution can be used to fix heavy metals in polluted soil, reduce their ability to enter plants, organisms and water bodies, thereby reducing their harm to the ecological environment.

Description

technical field [0001] The invention relates to the fields of environmental science and geotechnical engineering, in particular to microbial technology and the application of heavy metal solidification technology in soil, and in particular to a ferrobacteria and a method for removing heavy metal in soil by using it. Background technique [0002] In recent years, the impact of high-intensity human activities on the soil environment has become more and more obvious. With the intensification of industrial and urban pollution and the increase in the types and quantities of agricultural chemicals used, the problem of heavy metal pollution in the soil environment has become increasingly serious, especially in my country. In the Yangtze River Delta, where resources are relatively scarce and the economy is developing rapidly, the problem of soil heavy metal pollution cannot be ignored. Soil heavy metal pollution has the characteristics of poor mobility of pollutants in the soil, long...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/06
CPCB09C1/10C12N1/205C12R2001/06
Inventor 许朝阳吕惠周锋孟涛马耀仁
Owner YANGZHOU UNIV
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