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A method for improving microbial fermentation to produce 2,3-butanediol

A microbial fermentation, butanediol technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increased 2,3-butanediol production, long fermentation period, and inconspicuousness, and achieve High-efficiency glucose decomposition ability, accelerated glucose decomposition, and high-efficiency EMP efficiency

Active Publication Date: 2018-04-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN200910046726.8 discloses a Klebsiella pneumoniae and a method for preparing hydrogen and 2,3-butanediol, which ferment and co-produce hydrogen and 2,3-butanediol under anaerobic conditions, increasing the The added value of production, but the yield of 2,3-butanediol is very low
CN200710021641.5 discloses a Klebsiella oxytoca and its application, using the Klebsiella oxytoca to biosynthesize 2,3-butanediol and reduce the production of by-product organic acids in the process, but the The increase of 2,3-butanediol production was not obvious before and after treatment
CN200810138838.1 discloses a strain of Klebsiella pneumoniae and its application in the preparation of 2,3-butanediol, but the conversion of 2,3-butanediol with glucose as a substrate belongs to the oxidative metabolic pathway. Lebsiella is a typical facultative anaerobic microorganism, and the biomass accumulation is slow in the early stage of fermentation, so that the fermentation cycle is longer
CN200410037692.3 discloses a method for the production of 1,3-propanediol and 2,3 butanediol from glycerol by a microbial two-stage fermentation method, which adopts a new process of anaerobic two-stage fermentation in the early stage and aerobic stage in the later stage, although the anaerobic and good Oxygen condition control can artificially regulate the metabolic process of fermentation strains, but for the fermentation process of a single fermentation strain, such regulation effect is general, and more fermentation by-products will be introduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Seed liquid culture: Klebsiella bacteria preserved in liquid ( Klebsiella pneumoniae ) Add 1mL of the strain preservation solution to 400mL of LB medium, mix it in a 1L seed tank, and carry out seed liquid culture. The culture conditions were controlled as follows: the culture temperature was 37°C, the stirring speed was set at 200 rpm, the pH was controlled at 7.0, and micro-aerobic was maintained during the culture process.

[0026] Take the liquid preserved Lactobacillus casei ( Lactobacillus casei ) Add 1mL of the strain preservation solution to 400mL of MRS medium, mix it in a 1L seed tank, and carry out seed liquid cultivation. The culture conditions were controlled as follows: the culture temperature was 35 °C, the stirring speed was set at 200 rpm, the pH was controlled at 6.0, and micro-aerobic was maintained during the culture process.

[0027] (2) Fermentation culture: Batch fermentation mode is adopted, the volume of the fermentation tank is 15L, and...

Embodiment 2

[0033] (1) Seed liquid culture: Klebsiella bacteria preserved in liquid ( Klebsiella pneumoniae ) 1mL of strain preservation solution was added to 600mL of LB medium, and mixed in a 1L fermenter for seed liquid cultivation. The culture conditions were controlled as follows: the culture temperature was 37° C., the stirring speed was set at 300 rpm, the pH was controlled at 6.5, and micro-aerobic was maintained during the culture process.

[0034] Take the liquid preserved Lactobacillus casei ( Lactobacillus casei ) 1mL of strain preservation solution was added to 300mL of MRS medium, and mixed in a 1L fermenter for seed liquid cultivation. The culture conditions were controlled as follows: the culture temperature was 35°C, the stirring speed was set at 100 rpm, the pH was controlled at 6.0, and micro-aerobic was maintained during the culture process.

[0035] (2) Fermentation culture: Batch fermentation mode is adopted, the volume of the fermentation tank is 15L, and the vo...

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Abstract

The invention provides a method for improving microbial fermentation to produce 2,3-butanediol, comprising: (1) Seed liquid culture: use MRS medium to cultivate lactic acid bacteria to obtain lactic acid bacteria seed liquid; use LB medium to carry out Klebsiella Bacteria culture to obtain Klebsiella seed liquid; (2) Fermentation culture: Add the fermented seed liquid mixed with two strains into the fermentation medium, and anaerobic or microaerobic fermentation is the main method in the initial stage of fermentation. When the optical density OD650 is greater than 5, increase the ventilation and carry out aerobic fermentation. In the present invention, Klebsiella and lactic acid bacteria are co-fermented to produce 2,3-butanediol. On the one hand, lactic acid bacteria are used to optimize sugar metabolism, increase the supply of intermediate metabolites, and improve the fermentation level of 2,3-butanediol; On the one hand, by regulating the supply of oxygen during the fermentation process and regulating the metabolic pathways of the two bacteria, while increasing the production of 2,3-butanediol, high-concentration diacetyl with better economic benefits can be obtained.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for improving the production of 2,3-butanediol by fermentation bacteria through co-fermentation of two microorganisms. Background technique [0002] 2,3-butanediol can be used as a potential platform compound to replace traditional platform compounds for large-scale synthesis of methyl ethyl ketone (excellent solvent) and 1,3-butadiene (widely used in synthetic rubber, polyester and Polyurethane and other fields). In addition, 2,3-butanediol can also be used to prepare inks, perfumes, fumigants, moisturizers, softeners, plasticizers, explosives and pharmaceutical chiral carriers, etc., 2,3-butanediol Oxidation products 3-hydroxybutanone and diacetyl can be used as edible spices; at the same time, because of their high calorific value, which is equivalent to ethanol and methanol, they can be used as fuel additives; 2,3-butanediol can be produced after ester...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/18C12R1/245C12R1/22
Inventor 张霖廖莎姚新武师文静
Owner CHINA PETROLEUM & CHEM CORP
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