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Method for combined production of mannitol and D-lactic acid by virtue of microorganism anaerobic fermentation

An anaerobic fermentation and mannitol technology, applied in the biological field, can solve problems such as difficulty in utilization, waste residue pollution, etc., and achieve the effects of significant economic and social benefits, improving crystallization yield, and improving product quality and yield.

Inactive Publication Date: 2015-05-27
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to improve the D-lactic acid output, these methods add calcium salt to neutralize lactic acid to control pH in the fermentation process. The high-concentration calcium salt brings a lot of pressure to the follow-up separation, and the calcium sulfate of by-product is due to containing a large amount of pigment and Impurities are difficult to use and cause serious waste residue pollution

Method used

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  • Method for combined production of mannitol and D-lactic acid by virtue of microorganism anaerobic fermentation
  • Method for combined production of mannitol and D-lactic acid by virtue of microorganism anaerobic fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Seed liquid culture:

[0043] Using fructose syrup as the carbon source, the total sugar is 20g / L (the mass ratio of fructose to glucose is 3:1).

[0044] Primary seed culture: Inoculate the Leuconostoc pseudoenterolis G123 bacterial solution stored at -80°C at 1% v / v in the primary seed medium, and cultivate aerobically at 30°C and 200rpm for 12 hours.

[0045] The formula of the primary seed medium is: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, sodium acetate 5g / L, triammonium citrate 2g / L, dipotassium hydrogen phosphate trihydrate 2g / L L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Tween-801mL / L, with fructose syrup as carbon source (wherein the mass ratio of fructose to glucose is 3:1), total sugar 20g / L, the medium was sterilized at 121°C for 20min.

[0046] Secondary seed culture: transfer from the primary seed solution to the secondary seed medium with an inoculum of 5% v / v, 30°C, 200rpm, sterile air ventilation ...

Embodiment 2

[0054] (1) Seed liquid culture:

[0055] Using fructose syrup as the carbon source, the total sugar is 20g / L (the mass ratio of fructose to glucose is 1:1).

[0056] Primary seed culture: Inoculate the Leuconostoc pseudoenterolis G123 bacterial solution stored at -80°C at 1% v / v in the primary seed medium, and cultivate aerobically at 30°C and 200rpm for 12 hours.

[0057] The formula of the primary seed medium is: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, sodium acetate 5g / L, triammonium citrate 2g / L, dipotassium hydrogen phosphate trihydrate 2g / L L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Tween-801mL / L, with fructose syrup as carbon source (the mass ratio of fructose and glucose is 1:1), total sugar 20g / L, the medium was sterilized at 121°C for 20min.

[0058] Secondary seed culture: transfer from the primary seed solution to the secondary seed medium with an inoculum of 5% v / v, 30°C, 200rpm, sterile air ventilation of 0.1v...

Embodiment 3

[0064] (1) Seed liquid culture:

[0065] Using fructose syrup as the carbon source, the total sugar is 20g / L (the mass ratio of fructose to glucose is 2:1).

[0066] Primary seed culture: Inoculate the Leuconostoc pseudoenterolis G123 bacterial solution stored at -80°C at 1% v / v in the primary seed medium, and cultivate aerobically at 30°C and 200rpm for 12 hours.

[0067] The formula of the primary seed medium is: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, sodium acetate 5g / L, triammonium citrate 2g / L, dipotassium hydrogen phosphate trihydrate 2g / L L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Tween-801mL / L, with fructose syrup as carbon source (wherein the mass ratio of fructose and glucose is 2:1), total sugar 20g / L, the medium was sterilized at 121°C for 20min.

[0068] Secondary seed culture: transfer from the primary seed solution to the secondary seed medium with an inoculum of 5% v / v, 30°C, 200rpm, sterile air ventilation...

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Abstract

The invention discloses a method for combined production of mannitol and D-lactic acid by virtue of microorganism anaerobic fermentation and belongs to the technical field of biology. The mannitol and D-lactic acid are produced by adopting Leuconostoc pseudomesenteroides G 123 with the preservation number of CCTCC NO:M 2014115 under the condition of anaerobic fermentation. The method comprises the following steps: (a) carrying out fermentation on the strain in a fermentation medium containing fructose syrup to obtain a fermentation broth containing the mannitol and D-lactic acid; and (b) separating the mannitol and D-lactic acid from the fermentation broth, and purifying. According to the method disclosed by the invention, the mannitol and D-lactic acid can be simultaneously produced with high efficiency, the total conversion rates of the mannitol and D-lactic acid to sugar reach 90 percent or more, the extraction yields both reach 83 percent or more, the purity of product is high, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for the co-production of mannitol and D-lactic acid by microbial anaerobic fermentation. Background technique [0002] Mannitol (D-Mannitol), also known as mannitol, has 6 hydroxyl groups and is a six-carbon sugar alcohol compound. The sweetness is only half that of sucrose. Compared with other sugars, mannitol has the characteristics of non-hygroscopic, suitable sweetness, low calorie, no caries, and no toxic and side effects. Flavoring agents and additives of various functional foods. Mannitol is widely used in the fields of medicine, food and chemical industry. It is the main raw material of sugar-free chewing gum sweetener or anti-sticking agent. At present, the market scale of mannitol has reached tens of thousands of tons. [0003] There are two main methods for traditional industrial production of mannitol: one is the natural extraction method, kelp con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12P7/18C07C27/26C07C31/26C07C29/76C07C51/42C07C59/08C12R1/01
CPCC12P7/18C12P7/56C07C29/76C07C51/42C07C31/26C07C59/08
Inventor 吴昊姜岷朱婧张敏马江锋韦策
Owner NANJING UNIV OF TECH
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