Method for increasing content of arachidonic acid in porphyridium
A technology of arachidonic acid and porphyridium, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as high cost, slow growth, and poor product quality
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[0013] Preparation of the culture medium in step 1. The composition of the culture medium: 1000 parts of boiled and cooled seawater, 3 parts of sodium nitrate, 1 part of urea, 1.5 parts of ammonium chloride, 1.5 parts of potassium dihydrogen phosphate, 2 parts of ammonium bicarbonate, and 0.01 parts of ferrous sulfate servings, vitamin B 1 0.02 parts, vitamin B 12 0.001 part, 0.004 part of biotin, 0.003 part of nicotinic acid, 0.001 part of α-naphthaleneacetic acid, 0.2 part of magnesium sulfate, Na 2 0.002 parts of EDTA. Take 2000 ml of this culture solution and put it into a 3000 ml Erlenmeyer flask, seal it with plastic wrap and a rubber band, shake it on a shaker for 15 minutes, and put it into a 4°C refrigerator for future use.
[0014] Step 2, inoculation and cultivation Get a clean Erlenmeyer flask of 1500 milliliters, pack 600 milliliters of the culture solution described in step 1, get vigorous growth, pollution-free, Porphyridium algae inoculation in the exponentia...
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