Method for increasing content of arachidonic acid in porphyridium

A technology of arachidonic acid and porphyridium, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as high cost, slow growth, and poor product quality

Inactive Publication Date: 2015-06-24
LINYI UNIVERSITY
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Problems solved by technology

[0003] Traditionally, large-scale cultivation of Porphyridium adopts open runway-type large ponds and Allen medium for cultivation. The light intensity and temperature are completely dependent on nature, which is volatile and does not meet the requirements of growth and fatty acid accumulation of Porphyridium at various stages, and cannot be controlled artificially. , slow growth, low yield, easy pollution, high cost, large basic investment, low content of fatty acids, especially arachidonic acid, and poor product quality

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  • Method for increasing content of arachidonic acid in porphyridium

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Embodiment Construction

[0013] Preparation of the culture medium in step 1. The composition of the culture medium: 1000 parts of boiled and cooled seawater, 3 parts of sodium nitrate, 1 part of urea, 1.5 parts of ammonium chloride, 1.5 parts of potassium dihydrogen phosphate, 2 parts of ammonium bicarbonate, and 0.01 parts of ferrous sulfate servings, vitamin B 1 0.02 parts, vitamin B 12 0.001 part, 0.004 part of biotin, 0.003 part of nicotinic acid, 0.001 part of α-naphthaleneacetic acid, 0.2 part of magnesium sulfate, Na 2 0.002 parts of EDTA. Take 2000 ml of this culture solution and put it into a 3000 ml Erlenmeyer flask, seal it with plastic wrap and a rubber band, shake it on a shaker for 15 minutes, and put it into a 4°C refrigerator for future use.

[0014] Step 2, inoculation and cultivation Get a clean Erlenmeyer flask of 1500 milliliters, pack 600 milliliters of the culture solution described in step 1, get vigorous growth, pollution-free, Porphyridium algae inoculation in the exponentia...

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Abstract

The invention discloses a method for increasing the content of arachidonic acid in porphyridium and belongs to the field of aquaculture. Traditionally, porphyridium is cultured by an Allen culture medium in an open runway type large pond, so the growth is slow, the yield is low, pollution is easy to cause, the cost is high, basic investment is high, the content of arachidonic acid is low and the product quality is poor. According to the method disclosed by the invention, an illumination incubator is adopted for culture; in the later period of the culture, the illumination intensity and the illumination temperature are increased to force the porphyridium to accumulate arachidonic acid; sodium nitrate, ammonium chloride, ammonium bicarbonate, nicotinic acid alpha-naphthylacetic acid, magnesium sulfate, Na2EDTA and the like are replenished, and nutritional salts are replenished in batches in the later period of the culture, so that the nutrition requirements of the porphyridium are satisfied, the toxicity caused to porphyridium cells because of over-high concentration of the nutritional salts in the early stage due to one-time addition of the nutritional salts is overcome, the growth speed and yield of the porphyridium are greatly increased, and the content of arachidonic acid in the porphyridium is increased by 120%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for increasing the arachidonic acid content of Porphyrococcus. Background technique [0002] Porphyridium cruentum belongs to Rhodophyta, Rhodophyceae, Porphyrides, Porphyridaceae, and Porphyridium genus. Single cells, often irregularly gathered together, covered by a thin film, multi-spherical, blood red or dark purple, with 1 axial star-shaped or irregular-shaped pigment body and 1 unsheathed protein nucleus, and Cell division and asexual reproduction. The cells are 10-24μm in diameter and distributed all over the world. The salinity adapts to a wide range, the suitable temperature is 13-31°C, the optimum temperature is 21-26°C, the pH 5.3-8.3 can grow normally, and 7.5 is the optimum. Porphyridium can produce many biologically active substances, such as phycobiliproteins, polyunsaturated fatty acids and exopolysaccharides. Protein accounts for about 50% of b...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王培磊
Owner LINYI UNIVERSITY
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