The preparation method of gardenia blue pigment

A technology of gardenia blue pigment and gardenia leaves, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex extraction process, high cost of raw materials, excessive material consumption, etc., and achieve simple process and high product quality. Low cost, flexible production effects

Active Publication Date: 2018-02-09
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gardenia blue pigment has been widely used in many fields, such as food and medicine, due to its natural source, safety, non-toxicity, good human compatibility, excellent physical and chemical properties, and remarkable dyeing effect since its appearance. Increasingly, however, the traditional preparation method is to extract from gardenia fruit, and gardenia fruit contains a large amount of interfering substances, resulting in complex extraction process, excessive material consumption, low yield, and high raw material cost, the product Extremely expensive, which limits the large-scale application of such ingredients and the development of related industries in my country in recent years

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The specific operation steps of preparing gardenia blue pigment from gardenia leaves are as follows:

[0062] 1. Preparation of Gardenia Leaf Medium

[0063] 200 Kg fresh gardenia ( Gardenia jasminoides Ellis) leaves were washed, dried, crushed and passed through a 10-mesh sieve, sterilized at 100°C for 100 minutes, cooled for later use, and a total of 200 Kg of gardenia leaf solid medium was prepared.

[0064] . Aspergillus liquid seed preparation

[0065] (1) Aspergillus niger ( Aspergillus niger ) was inoculated on SDAY slant medium, kept at a constant temperature of 25°C, and cultured for 4 days to obtain the activated stock;

[0066] (2) Inoculate the activated stock into the liquid seed medium

[0067] Each liter of liquid seed medium consists of 5 g of peptone, 10 g of starch, 1 g of potassium dihydrogen phosphate, 1 g of dipotassium hydrogen phosphate, and 1 g of magnesium sulfate, which are dissolved in water to 1 L.

[0068] Fill the triangular flask w...

Embodiment 2

[0078] The specific operation steps of preparing gardenia blue pigment from gardenia leaves are as follows:

[0079] 1. Preparation of Gardenia Leaf Medium

[0080] 200 kg of dried water gardenia with a moisture content of 20% ( Gardenia jasminoides var. radicans Makino ) leaves, crushed through a 100-mesh sieve, mixed with water for 5 minutes according to the material-to-liquid ratio of 1Kg:0.2L, sterilized at 120°C for 20 minutes, and cooled to obtain 240 kg of gardenia leaf solid medium;

[0081] 2. Aspergillus liquid seed preparation

[0082] (1) Aspergillus niger ( Aspergillus niger ) was inoculated on SDAY slant medium, and cultured at 35°C for 7 days to obtain the activated stock;

[0083] (2) Inoculate the activated stock into the liquid seed medium

[0084] Each liter of liquid seed medium is prepared by adding 30 g of yeast extract, 50 g of sucrose, 3 g of potassium dihydrogen phosphate, 3 g of dipotassium hydrogen phosphate, and 3 g of magnesium sulfate, and...

Embodiment 3

[0095] The specific operation steps of preparing gardenia blue pigment from gardenia leaves are as follows:

[0096] 1. Preparation of gardenia leaf culture medium

[0097] 200 kg of fresh gardenia ( Gardenia jasminoides Ellis) Gardenia leaves, crushed through a 50-mesh sieve, mixed with water for 30 minutes according to the material-to-liquid ratio of 1Kg:0.5L, sterilized at 110°C for 50 minutes, and cooled to obtain 300 kg of Gardenia leaf liquid culture medium;

[0098] 2. Aspergillus liquid seed preparation

[0099] (1) Aspergillus niger ( Aspergillus niger ) was inoculated on SDAY slant medium, kept at a constant temperature of 30°C, and cultured for 5 days to obtain the activated stock;

[0100] (2) Inoculate the activated stock into the liquid medium

[0101] Each liter of liquid seed medium is prepared by adding 15 g of yeast extract, 25 g of sucrose, 2 g of potassium dihydrogen phosphate, 2 g of dipotassium hydrogen phosphate, and 2 g of magnesium sulfate, and...

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PUM

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Abstract

The invention discloses a preparation method of gardenia blue pigment. The specific process steps are as follows: (1) preparation of gardenia leaf medium, including preparation of solid medium and liquid medium; (2) preparation of liquid seeds of Aspergillus, including preparation of primary, secondary and tertiary seeds; (3) inoculation Fermentation, including liquid-solid two-way fermentation and liquid fermentation; (4) Enzymatic hydrolysis of gardeniside, including liquid-solid two-way fermentation and liquid fermentation; (5) Gardenia blue pigment generation and purification. The gardenia aglycone and amino acid are condensed by adding amino acid to form gardenia blue pigment, and the pigment is purified by resin column, and finally a blue-black powder is obtained, which is the gardenia blue pigment with a color value greater than 190 (E1%1cm 590nm). The invention utilizes the gardenia leaves to produce the gardenia blue pigment, which greatly reduces the production cost; the process of the invention is simple and stable, the investment in the production equipment can be large or small, and the production is flexible, and is suitable for the investment of enterprises of various scales.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and in particular relates to a preparation method of gardenia blue pigment. Background technique [0002] Gardenia blue pigment was initially produced by hydrolyzing geniposide in gardenia fruit and reacting with amino acids. Gardenia blue pigment is soluble in water and low-alcohol and other hydrophilic solvents. It has excellent physical and chemical properties such as light and heat resistance, acid and alkali resistance, and unique dyeing effect. Gardenia blue pigment can not only replace synthetic pigments by itself, but can also be used in harmony with red and yellow pigments of other strains, and can be blended into a series of blue-green hues such as green, purple, and brown. Gardenia blue pigment has been widely used in many fields, such as food and medicine, due to its natural source, safety, non-toxicity, good human compatibility, excellent ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/685
Inventor 胡丰林
Owner ANHUI AGRICULTURAL UNIVERSITY
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