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Fermentation method capable of improving biological protein content of yellow maize straw

A technology of straw biology and protein content, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low protein content of yellow corn straw, difficulty in using grass-fed animal husbandry feed, energy protein imbalance, etc. Achieve the effect of improved palatability, significant effect and short fermentation period

Inactive Publication Date: 2015-07-08
原平市科学技术交流中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of low protein content, poor quality, unbalanced energy protein and low nutritional value of yellow corn stalks, the present invention provides a fermentation method for increasing the biological protein content of yellow corn stalks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A kind of fermentation method that improves the biological protein content of yellow corn stalks, the yellow corn stalks are fermented with the mutagenic bacteria strains irradiated with willow stalks ultraviolet rays, wherein:

[0019] 1.1 The preparation steps of the fungus are as follows: the fungus is collected from Changbai Mountain, purified and rejuvenated by ultraviolet rays in the laboratory, and the strains with high vigor are screened out to be used for fermentation of corn stalks;

[0020] 1.2 Culture medium preparation:

[0021] Mother seed medium: add 20g of potatoes, 2g of glucose, 0.3g of dipotassium hydrogen phosphate, 0.15g of magnesium sulfate, 10mg of VB, 1.5g of agar, pH=6.5 for every 100ml of water; conventional slant culture;

[0022] Original seed and production seed medium: 60% cottonseed hulls, 25% bran, 11% corn flour, 2% ammonium sulfate, 1.3% white sugar, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.2%, magnesium sulfate (Mg...

Embodiment 2

[0030] Embodiment 2: tile fermentation

[0031] A fermentation method for increasing the biological protein content of yellow corn stalks, the preparation method of the Agaricus salicifolia strain and the preparation of the culture medium are the same as those described in Example 1. Corn stalks are crushed with a particle size of 0.5-1cm; the specific proportions are 75% corn stalk powder, 20% bran, 4% corn flour, 0.5% urea, 0.2% dipotassium hydrogen phosphate (K2HPO4), 0.3% magnesium sulfate (MgSO4) ;Adjust the pH=6.5-7.0; add water until the water content of the raw materials reaches 60%-65%, mix the raw materials well, cover them with plastic film after accumulation, and sterilize them by natural fermentation and heating for 24 hours. Or lay the fermentation bed flat for fermentation, cool the raw materials before inoculation, break the production seeds into 1-2cm bacterial blocks, and spread the bacterial seeds evenly in the raw materials when the temperature of the raw m...

Embodiment 3

[0032] Embodiment 3: frame type fermentation

[0033] A fermentation method for increasing the biological protein content of yellow corn stalks, the preparation method of the Agaricus salicifolia strain and the preparation of the culture medium are the same as those described in Example 1. The processed raw materials are inoculated while being framed, and the seeding rate is 8%-10% of the raw materials; frame-type fermentation requires 5-6 layers of fermentation beds, and the fermentation baskets are placed on the fermentation bed. Multi-layer frame-type fermentation has good air permeability. The fermentation process will generate heat, which is conducive to maintaining a higher temperature, but avoid excessive temperature. At the same time, pay attention to spraying water to increase humidity. Ferment at 28°C for about 8 days, and the culture medium can be stored in the sun when the mycelium is overgrown.

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Abstract

The invention belongs to the technical field of fermented straw feed and provides a fermentation method capable of improving the biological protein content of yellow maize straw for solving the problems that the yellow maize straw is low in protein content, poor in quality, unbalanced in protein and low in nutritive value, and can not be easily used as herbivorous livestock feed. The method comprises the steps of fermenting the yellow maize straw by using ultraviolet irradiation induced strain of marasmius salicicola, wherein the marasmius salicicola is picked from Changbai mountains, wherein ultraviolet purification and rejuvenation cultivation are carried out in a laboratory to screen out high-activity strains for the fermented maize straw strain. In the high-activity strains, the enzyme activity of carboxymethyl cellulase is detected to be 167.0mmol / ml; the mycelium is thick and strong; through plate cultivation, the strains can grow all over the whole plate one day earlier than oyster mushrooms and shiitake mushrooms; the varietal characteristic query and laboratory analysis prove that the strains are non-toxic. The fermented maize straw is white yellow, the fermented material is firm in fungus blocks, and the white mycelium is thick and has strong mushroom flavor; the biological protein (true protein) content is at least improved by 2%, and the true protein content averagely reaches 6.87%; the fermented feed is good in effect when used for fattening lambs.

Description

technical field [0001] The invention belongs to the technical field of straw fermented feed, and in particular relates to a fermentation method for increasing the biological protein content of yellow corn straw. Background technique [0002] With the development of my country's economy, people's living standards continue to improve, and the demand for meat, eggs, and milk is increasing day by day. The proportion of feed grains in the country's total grain output has risen from about 20% in the early 1980s to more than 40% now; With little land and a huge shortage of feed and grain, it is an important decision for national food security to develop grass-fed livestock by using straw and dregs to ensure the supply of high-quality meat. It is estimated that compared with raising pigs, every 10 million tons of beef and mutton produced can save 80 million tons of grain. However, the population continues to increase, the arable land continues to decrease, and the serious shortage o...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14C12N15/01C12N13/00C12N1/14C12R1/645
CPCY02P60/87
Inventor 张建新王小军安建云王士礼赵宇琼赵俊星任有蛇张春香项斌伟
Owner 原平市科学技术交流中心
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