Preparation method of a novel anticoagulant stent coating for capturing endothelial progenitor cells EPCs

An endothelial progenitor cell and anti-coagulation technology, which is applied in the field of biomedical engineering materials, can solve the problems of poor anti-coagulation performance and blood compatibility, and achieve the effect of excellent anti-coagulation ability, strong application potential, and small raw material input

Inactive Publication Date: 2017-10-24
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in most cases, while endothelialization is often promoted, the anticoagulant performance and blood compatibility of the implanted material surface are poor, such as extracellular matrix proteins, CD133, etc., so we also need to co-graft a Biomolecules with excellent biocompatibility

Method used

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  • Preparation method of a novel anticoagulant stent coating for capturing endothelial progenitor cells EPCs
  • Preparation method of a novel anticoagulant stent coating for capturing endothelial progenitor cells EPCs
  • Preparation method of a novel anticoagulant stent coating for capturing endothelial progenitor cells EPCs

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Experimental program
Comparison scheme
Effect test

Embodiment approach 2

[0033] 1. Prepare a sample grafted with CD133 using a gold sheet;

[0034] 2. Then wash the QCM-D channel with SDS and UP water in sequence;

[0035] 3. Place the sample, then open the software, set the temperature to 37°C, and test the sensitivity of the instrument;

[0036] 4. Start to run the air to check whether the QCM-D gold sheet is intact; then pass into the PBS buffer

[0037] 5. After the baseline runs flat, inject αMEM (containing 10% bovine serum albumin) medium solution for about 30 minutes to obtain a relatively flat baseline;

[0038] 6. Pass through the EPCs cell solution containing bovine serum albumin (the cell density is about 10^4 / ml); finally wait for about 8 hours, and then observe the changes in frequency and dissipation. After the experiment is over, analyze the data.

Embodiment 1

[0040] 1. The titanium oxide stent was ultrasonically cleaned three times with SDS, alcohol (ethanol), and RO water (deionized water) for 10 minutes each time, and then dried;

[0041] 2. Hydroxylation treatment of slides: Put the slides in concentrated sulfuric acid and hydrogen peroxide according to the volume ratio of 7:3 (V%:V%=7:3) and soak in 100°C for 2 hours; then wash with RO water for 3 hours times, 5 minutes each time;

[0042] 3. Then immediately put the above sample into Tris-base (10mM, pH8.5) buffer solution, put it in a shaker and shake it tightly for about 1 hour, and then shake it open for about 11 hours. Then use RO water to ultrasonically clean 3 times, and dry with cold air.

[0043] 4. Then configure 10ml of EDC / NHS / MES (1omM / 20mM / 50mM) PBS solution with pH 5.4, then dilute the concentration of CD133 solution with EDC to 0.8-3.2ug / ml and the concentration of Fucoidan to 50-400ug / ml; 50ul each of the CD133 solution and Fucoidan were dropped onto the surf...

Embodiment 2

[0045] 1. The titanium oxide stent was ultrasonically cleaned three times with SDS, alcohol (ethanol), and RO water (deionized water) for 10 minutes each time, and then dried;

[0046] 2. Hydroxylation treatment of slides: Put the slides in concentrated sulfuric acid and hydrogen peroxide according to the volume ratio of 7:3 (V%:V%=7:3) and soak in 100°C for 2 hours; then wash with RO water for 3 hours times, 5 minutes each time;

[0047] 3. Immediately put the above sample into Tris-base (10mM, pH8.5) buffer solution, soak for 8h, then ultrasonically clean it with RO water for 3 times, each time for 5min, and then continue to soak for 3 times in a row. The glass slides of the dopamine film are sealed and preserved;

[0048] 4. Then configure 10ml of EDC / NHS / MES (5mM / 10mM / 50mM) PBS solution with pH 7.4, then dilute the concentration of CD133 solution with EDC to 0.8-3.2ug / ml and the concentration of Fucoidan to 50-400ug / ml; 50ul of CD133 solution and Fucoidan were dropped on...

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Abstract

The invention discloses a preparation method of a novel anticoagulant stent coating for capturing endothelial progenitor cells EPCs, the main steps of which are to carry out hydroxylation treatment on the surface of the material, and then in Tris-base (pH8.5, 2mg / ml) Dissolve dopamine in the solution, then deposit one or more layers of dopamine film on the surface of the material at 37°C, then adjust the reaction conditions according to the pH and EDC / NHS concentration, and finally covalently graft CD133+ (0.8-3.2ug / ml ) and Fucoidan (50‑400ug / ml) to obtain the target product. The invention has the advantages of simple operation, mild reaction conditions, high efficiency and low cost. Due to the effect of low-molecular-weight fucoidan, its target coating has strong anti-thrombosis, anti-coagulation and inhibition of smooth muscle intimal hyperplasia, and can quickly capture endothelial progenitor cells in peripheral blood, thereby inducing their differentiation into Endothelial cells, forming a complete endothelial cell layer, have excellent anticoagulant properties and biocompatibility.

Description

technical field [0001] The invention relates to a biomedical engineering material, especially a multifunctional coating for capturing stem cells and endothelial progenitor cells EPCs with good blood compatibility. Background technique [0002] According to the WHO report, cardiovascular disease has become the main killer of people's death. PCI stent interventional surgery is a relatively mature and effective method, which can greatly relieve people's suffering and reduce mortality. However, after interventional surgery, restenosis and late thrombosis still have a rate of up to 30% 3-6 months after stent intervention, which is mainly due to the lack of biocompatibility and functionality of the stent surface, which cannot be quickly treated. Formation of a complete and continuous endothelial layer. The inner layer of blood vessels is mainly composed of a single layer of endothelial cells and basement membrane, and the middle layer is mainly composed of smooth muscle cells. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L33/08A61L31/08
Inventor 赵安莎薛国能黄楠杨苹王艳
Owner SOUTHWEST JIAOTONG UNIV
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