Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for extracting and preparing high-purity raffinose from degreased wheat germ

A raffinose, high-purity technology, applied in the field of chemical engineering, can solve the problems of low extraction rate, limited industrial scale and efficiency, and prolonged crystallization time, and achieve the effect of simple and reliable process, convenient parameters, and easy realization

Active Publication Date: 2015-07-22
ZHEJIANG UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some problems in the aspect of industrialization, mainly: the amount of solvent used in the static leaching method is large, the extraction rate is not high, generally it takes a long time and repeated extractions, and the requirements for the equipment for stirring extraction are also relatively high; static adsorption decolorization in the alcohol system, activated carbon The consumption is large, and the activated carbon powder is difficult to reuse; because the purity of raffinose is not ideal, obtaining raffinose with a purity of more than 98% requires multiple crystallizations, which will lead to low yields of raffinose, crystallization Prolonged time, which also limits industrial scale and efficiency
[0008] Therefore, the existing technology for extracting and purifying raffinose from defatted wheat germ still has certain shortcomings and is not suitable for industrial production. It needs to be improved in terms of cost control and yield.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for extracting and preparing high-purity raffinose from degreased wheat germ
  • Process for extracting and preparing high-purity raffinose from degreased wheat germ
  • Process for extracting and preparing high-purity raffinose from degreased wheat germ

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Weigh 200 g of defatted wheat germ (wherein the mass percentage of raffinose is 4.6%), and fill it into a percolation column (Φ4.0×50 cm) by wet method. Soak at a constant temperature of ℃ for 30 minutes to make it fully swell, open the outlet valve of the percolation column, and at the same time continuously add 80% ethanol aqueous solution from the top of the column, control the flow rate at 18-20mL / min, until the volume of the percolation liquid collected is 1371mL.

[0063] Weigh 230g of defatted wheat germ, and after wet packing evenly, its accumulation volume is 592mL, and other conditions are the same as above, and the volume of collected percolate is 1948mL.

[0064] Weigh 150g of defatted wheat germ, and after wet filling evenly, its accumulation volume is 355mL, and other conditions are the same as above, and the volume of collected percolate is 1066mL.

[0065] The above three groups of percolation liquids were combined as the raw material liquid for subs...

Embodiment 2

[0072] Take 500 mL of the extract in (1), and the operation method of (2) is the same as in Example 1. After analysis, the purity of the raffinose in the water phase is 28.7%, and the solid content concentration is 100.7 mg / mL.

[0073] (3) with 5wt% Na 2 SO 4 As extreme water, the conditions of electrodialysis are: voltage 25V, flow rate of each material 15L / h. After 2.5 hours of electrodialysis desalination, the conductivity of the desalination chamber decreased from the initial 992 μS / cm to 108 μS / cm.

[0074] (4) Simulated moving bed separation: The simulated moving bed device is composed of 8 pieces of 25cm*0.46cm stainless steel filled with sodium-type strong ion exchange resins of 200-400 mesh and a cross-linking degree of 4% in series. The mouth is passed into the simulated moving bed device at a flow rate of 0.1mL / min, and the flow rate of each zone is: flow rate of zone I (0.75), flow rate of zone II (0.47), flow rate of zone III (0.51), flow rate of zone IV (0.5) ...

Embodiment 3

[0077] (1), (2) operation method is the same as embodiment 1.

[0078] (3) with 5wt% Na 2 SO 4 As extreme water, the conditions of electrodialysis are: voltage 25V, flow rate of each material 15L / h. After 2.5 hours of electrodialysis desalination, the conductivity of the desalination chamber decreased from the initial 1017 μS / cm to 115 μS / cm.

[0079] (4) Simulated moving bed separation: The simulated moving bed device is composed of 8 pieces of 25cm*0.46cm stainless steel filled with calcium-type strong ion exchange resins of 100-200 mesh and a cross-linking degree of 2% in series. The mouth is passed into the simulated moving bed device at a flow rate of 0.1mL / min, and the flow rate of each zone is: flow rate of zone I (0.75), flow rate of zone II (0.47), flow rate of zone III (0.51), flow rate of zone IV (0.5) . The switching time is 60s, and the separation temperature is 65°C. The raffinose-containing raffinose phase obtained from the raffinate port has a purity of 91...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a process for extracting and preparing high-purity raffinose from degreased wheat germ. The degreased wheat germ is taken as a raw material for percolation and extraction, a raffinose extracting solution is obtained, and discolored through extraction, an extraction solution is obtained and subjected to electrodialysis and desalting treatment, the desalted solution is obtained, impurities in the desalted solution are further removed by the aid of a simulated moving bed, raffinose is concentrated, oversaturated syrup is obtained, finally, the oversaturated syrup of the raffinose is subjected to crystallization treatment, ultimately, the high-purity raffinose with the purity higher than 98% is obtained, and the yield is higher than 75%. The whole process is simple, reliable and easy to operate and implement, and parameters are convenient to control.

Description

technical field [0001] The invention belongs to the technical field of chemical engineering, in particular to a process flow for extracting high-purity raffinose from defatted wheat germ. Specifically, high-purity raffinose is extracted and separated from defatted wheat germ by using combined separation processes such as percolation extraction, extraction decolorization, electrodialysis desalination, simulated moving bed separation and crystallization. Background technique [0002] It is a functional oligosaccharide widely present in natural products, and its standard name is O-α-D-galactopyranosyl (1→6)-α-D-glucopyranosyl (1→2) -β-D-fructofuranose. It is a non-reducing sugar, formed by condensation of galactose, glucose and fructose. Its structural formula is as follows: [0003] [0004] Raffinose was once considered an anti-nutritional factor. The reason is that the human body lacks α-galactosidase, which prevents raffinose from being decomposed and absorbed in the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08
CPCC07H3/06C13K13/00B01D2311/04C07H1/08B01D15/1821B01D61/44Y02A20/124B01D2311/2643B01D2311/2697B01D2311/2649A23L5/23A23L5/30A23V2002/00A23V2250/622B01D71/08
Inventor 鲍宗必黄敏慧段舒然周倩倩张治国杨启炜苏宝根邢华斌任其龙杨亦文
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com