A drug screening method with high precision and high reproducibility

Abstract

The present invention provides a drug screening method with high accuracy and high repeatability. The method includes the following steps: 1) preparing a patterned fiber electrospun receiving plate, using photoetching technology and DC magnetron sputtering technology to prepare Receiver plate with patterned metal silver layer; 2) Prepare patterned electrospun fiber, use the result of step 1) for electrospinning; 3) Prepare PDMS cavity; 4) Use the result of step 2) and step 3) with Plasma treatment for connection; 5) Inoculate primary mouse cardiomyocytes on the patterned electrospun fibers in the result of step 4), use a syringe pump to pump the medium into the cavity of the result of step 4), and then inject the drug Pump in, record the beating frequency of cardiomyocytes within 20 days after the drug is pumped in, get the EC50 value, and brush the drug according to the EC50 value. The invention realizes the normal growth of cells in a small cavity, and at the same time has the effects of screening drugs and reusing them, and has high precision and high repeatability.

Description

technical field [0001] The invention belongs to the field of drug screening, in particular to a drug screening method with high accuracy and high repeatability. [0002] technical background [0003] Drug screening is to screen out highly effective new drugs or lead compounds from natural or synthetic compounds. The process of drug screening is the process of conducting drug activity experiments on compounds, and the activity experiments on compounds have gradually changed from early confirmatory experiments to screening experiments, which is an important step in testing and obtaining specific physiologically active compounds in the modern drug development process. step. [0004] In the prior art, the two-hybrid drug screening technology, the recombinant receptor drug screening technology and the transgenic animal model drug screening technology all have the disadvantages of complicated technical steps, high detection cost and poor accuracy. [0005] In recent years, chip d...

AI Technical Summary

Problems solved by technology

However, the chips in the prior art all have the problems of high processing difficulty, scarcity of raw materials or high cost of raw materials, making it difficult for drug screening chips to be widely used.

[0006] In particular, the use of electrospun fibers to prepare drug screening systems, especially chip screening systems, has always been faced with the technical problems that it is difficult to prepare a specific array of electrospun fibers, and it is difficult to fit the electrospun fibers to the size of the chip.

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