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Preparation method for mycelium nano composite pellet of core-shell structure

A nano-composite, core-shell structure technology, applied in the preparation of microspheres, microcapsule preparations, etc., can solve the problems of difficult recycling of nanoparticles, and achieve the effects of good biodegradability, increased specific surface area, and simple preparation process

Inactive Publication Date: 2015-07-29
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a method for preparing a core-shell mycelium nanocomposite ball; to solve the problem of difficult recycling of nanoparticles in practical applications, the invention is used to prepare the core-shell structure mycelium Nano-composite ball materials are low in cost, environmentally friendly, and easy to produce on a large scale

Method used

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  • Preparation method for mycelium nano composite pellet of core-shell structure

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Experimental program
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Effect test

Embodiment 1

[0021] A preparation method of nucleocapsid hypha nanocomposite spheres includes the following steps:

[0022] a. Preparation of culture medium: According to the raw material components and proportions containing 1 gram of glucose, 0.25 grams of peptone, 0.25 grams of yeast powder, and the balance of water per 100 ml of liquid medium, take the raw materials of each component, dissolve and mix evenly , And keep the pH constant, sterilize for 20 minutes under the conditions of a pressure of 103.4 kPa steam pressure and a temperature of 120 degrees Celsius to prepare a liquid culture medium; take 2 250 ml conical flasks and place them in each cone Add 125 ml of liquid culture medium to the flask;

[0023] b. Preparation of mycelium / nanoparticle A composite ball: Add nano tetroxide with a concentration of 1 mg of ferroferric oxide (ie nanoparticle A) per ml of solution to the sterilized liquid medium in an Erlenmeyer flask Mix 4 ml of tri-iron aqueous solution, mix well, then add 0.1 ...

Embodiment 2

[0027] A preparation method of nucleocapsid hypha nanocomposite spheres includes the following steps:

[0028] a. Preparation of culture medium: According to the raw material components and proportions containing 1 gram of glucose, 0.25 grams of peptone, 0.25 grams of yeast powder, and the balance of water per 100 ml of liquid medium, take the raw materials of each component, dissolve and mix evenly , And keep the pH constant, sterilize for 20 minutes under the conditions of a pressure of 103.4 kPa steam pressure and a temperature of 120 degrees Celsius to prepare a liquid culture medium; take 2 250 ml conical flasks and place them in each cone Add 125 ml of liquid culture medium to the flask;

[0029] b. Preparation of mycelium / nanoparticle A composite ball: Add nanographite oxide with a concentration of 1 mg of nanographene oxide (ie nanoparticle A) per ml of solution to the sterilized liquid medium in an Erlenmeyer flask Mix 4 ml of alkene aqueous solution, mix well, then add 0...

Embodiment 3

[0033] A preparation method of nucleocapsid hypha nanocomposite spheres includes the following steps:

[0034] a. Preparation of culture medium: According to the raw material components and proportions containing 1 gram of glucose, 0.25 grams of peptone, 0.25 grams of yeast powder, and the balance of water per 100 ml of liquid medium, take the raw materials of each component, dissolve and mix evenly , And keep the pH constant, sterilize for 15 minutes under the conditions of 103.4 kPa steam pressure and 120 degrees Celsius to obtain liquid culture medium; take 2 250 ml conical flasks and place them in each cone Add 125 ml of liquid culture medium to the flask;

[0035] b. Preparation of mycelium / nanoparticle A composite ball: Add a carbon nitride aqueous solution with a concentration of 2 mg carbon nitride (ie nanoparticle A) per ml of solution to the sterilized liquid medium in an Erlenmeyer flask 10ml, mix well, then add 0.1g of fungal strains, culture for 50 hours under the con...

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Abstract

The invention discloses a preparation method for a mycelium nano composite pellet of a core-shell structure. The preparation method is characterized by comprising the following steps: preparing a fluid medium; adding a nano-particle A aqueous solution into the sterilized fluid medium in a container, uniformly mixing, inoculating a fungi strain, and culturing for 48-72 h at the temperature of 15-35 DEG C under rotating oscillation with the rotating speed of 80-200 r / m so as to form a mycelium / nano particle A composite pellet; putting the mycelium / nano particle A composite pellet into a nano particle B fluid medium for culturing for 24-48 h under rotating oscillation so as to form the mycelium nano composite pellet of the core-shell structure; soaking the composite pellet with a sodium hydroxide aqueous solution for 12 h, washing the composite pellet to be neutral by deionized water, and carrying out vacuum freeze-drying so as to obtain the mycelium nano composite pellet of the core-shell structure. The composite pellet material provided by the invention has good biodegradability, adsorbability, thermal stability and a good mechanical property, and can be widely used for catalysis, adsorption, enrichment, separation and recycling of radionuclide, heavy metal ions and dyes, water treatment and the like.

Description

Technical field [0001] The invention belongs to the preparation of organic and inorganic nano composite materials, and relates to a preparation method of a core-shell structure hyphae nano composite ball. The core-shell structure mycelium nano composite ball material prepared by the present invention integrates many excellent properties such as organic and inorganic materials, has good biodegradability, adsorbability, thermal stability and mechanical properties, and can be widely used in catalysis, and Chemical fields such as the adsorption, enrichment, separation and recovery of radionuclides, heavy metal ions and dyes, and water treatment. Background technique [0002] Nanomaterials have been paid attention to in physics, chemistry, materials, biology and other disciplines due to their many excellent characteristics compared with traditional materials. However, the small particle size of the past nanomaterials makes recycling technology difficult, so This leads to an increase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02
CPCB01J13/02
Inventor 竹文坤段涛姚卫棠周建罗学刚
Owner SOUTHWEAT UNIV OF SCI & TECH
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