Immunocyte culture medium, and culture method and application of immunocytes

A technology of immune cells and medium, which is applied in the field of cell culture, can solve the problems of slow cell expansion, achieve good immune cell function, and prolong the number of passages

Inactive Publication Date: 2015-08-05
BIOTOWNTEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the prior art above, the purpose of the present invention is to provide a culture medium, cultur

Method used

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  • Immunocyte culture medium, and culture method and application of immunocytes
  • Immunocyte culture medium, and culture method and application of immunocytes

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Experimental program
Comparison scheme
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specific Embodiment

[0036] The composition of the immune cell culture medium (calculated in 1 liter) according to the present invention is shown in the following table:

[0037] Table 1. Basic medium composition

[0038] Element concentration or amount Ala (alanine) 0.05 mM Arg (arginine) 2.00 mM Asn (asparagine) 0.55 mM Asp (aspartic acid) 0.4 mM Cys (cysteine) 0.15 mM Glu (glutamic acid) 0.15 mM Gly (Glycine) 0.25 mM His (histidine) 0.15 mM Ile (Isoleucine) 2.50 mM Leu (leucine) 1.20 mM Lys (lysine) 1.00 mM Met (methionine) 0.60 mM Phe (phenylalanine) 1.05 mM Pro (proline) 0.02 mM Ser (serine) 0.70 mM Thr (threonine) 0.30 mM Trp (tryptophan) 0.10 mM Tyr (tyrosine) 0.15 mM Val (valine) 1.4 mM Gln (glutamine) 4 mM Insulin 2-10mg / L Transferrin (transferrin) 5-50mg / L Human Serum Albumin (HSA) 0.1g / L ascorbic acid 50mg / L...

Embodiment 1

[0042] Example 1. Accelerating effect on T cell proliferation

[0043] In order to check the influence of the culture medium described in the present invention on the expansion speed of T cell culture, the T cells of human origin are selected for use in vitro cell culture experiments, and the cells are pressed by 10 4 After inoculation, conventional culture medium (control group) and immune cell culture medium of the present invention (experimental group) were added for culture (37°C, 5% CO 2 ), and the cell density was measured by MTT method at 72 hours. The results of the study show that the expansion speed of T cells using the cell culture medium described in the present invention is compared with the conventional medium (Takara GT-T551), and the cell proliferation speed is increased by 3.8 times under the same conditions (see figure 2 ).

[0044] T cells cultured through the immune cell culture medium of the present invention ( figure 2 Middle solid column) T cells cu...

Embodiment 2

[0045] Example 2 Acceleration of NK cell proliferation

[0046] In order to check the influence of the immune cell culture medium of the present invention on the expansion speed of NK cell culture, select NK cells from mammalian organisms such as people, mice, rats to do in vitro cell culture experiments, and the cells are divided into 10 4 After inoculation, conventional culture medium (control group) and immune cell culture medium (experimental group) of the present invention were added to culture (37°C, 5% CO 2 ), and the cell density was measured by MTT assay after 72 hours. The research results show that, compared with the conventional culture medium (Takara GT-T551), the expansion speed of NK cells using the immune cell culture medium of the present invention is increased by 3.2 times under the same conditions (see image 3 ).

[0047] The NK cells (solid column) cultured by the immune medium of the present invention show a faster expansion rate than the NK cells (hol...

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Abstract

The invention discloses an immunocyte culture medium, and a culture method and application of immunocytes. The immunocyte culture medium is composed of one or more selected from the group consisting of amino acids, vitamins, salts, lipids or polypeptide, and one or more selected from the group consisting of cytokines or antibodies; the immunocyte culture medium can quickly amplify different types of immunocytes, enables the amplification speed of the immunocytes to be increased by 3 to 5 times than the amplification speed of the immunocytes cultured with a conventional culture medium, and prolongs passage times; meanwhile, the immunocytes cultured by the immunocyte culture medium provided by the invention can maintain good immunocyte functions and exert effects including, but not limited to, anti-cancer effect and anti-aging effect, so the immunocytes have good application prospects.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to an immune cell culture medium, culture method and application. Background technique [0002] Many studies have shown that immune cells are not only an important natural defense system for the body to prevent foreign body infection, but also play an important role in the body's emergency response to fighting tumor cells. There are many types of immune cells, including but limited to T cells, B cells, NK cells, DC cells, and CIK cells. In recent years, many studies have shown that the rapid expansion of immune cells in an emergency state is the response of the immune response to environmental changes, thereby playing the role of phagocytosis, killing and removal of foreign substances. However, for cancerous tissues, tumor cells can have the characteristics of immune escape, that is, they can evade the normal immune defense system, and then grow excessively in the body to form tumor tis...

Claims

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Application Information

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IPC IPC(8): C12N5/078C12N5/0783C12N5/0784
Inventor 刘小青
Owner BIOTOWNTEK CO LTD
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